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DOI :
10.3791/1167-v
March 6th, 2009
Chapters
0:00
Title
0:09
Introduction
3:21
Reagents and Equipment
5:44
Assemble Materials and Equipment at Workspace
6:04
Step 1: PBMC Isolation
8:51
Step 2: CD4+ Cell Separatioin
11:01
Step 3: In Vitro Expansion Culture
13:34
Step 4: Visualizing T Cells by Tetramer Staining
15:33
Conclusion
1:05
T Cell Staining
この手順では、精製および抗原特異的CD4 + MHCクラスIIテトラマーを用いて全末梢血とその可視化から、T細胞のin vitroでの展開の例を示します。四量体は、単一の抗原特異性と定義されているMHCクラスII制限とT細胞の直接可視化を可能にする。
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