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DOI :
10.3791/1178-v
May 18th, 2009
Chapters
0:00
Title
1:01
Introduction
1:35
Subcloning, Expressing, and Binding a GST-Fusion protein to GLU-coated beads
6:41
The GST-fusion Protein Binding Assay
9:12
Preparing gel-separated proteins for Mass Spectrometry
15:33
Representative Results/ Outcome
16:03
Conclusion
우리는 ATP - 문이 P2X2 수용체의 전체 길이 C 터미 너스에 바인딩 뇌 단백질을 식별하는 간단한 프로토콜을 설명합니다. 모든 P2X 수용체에 대한 확장 및이 방법의 체계적인 응용 프로그램은 P2X 수용체 신호의 더 나은 이해로 이어질 것으로 예상된다.
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