La préparation des Drosophile Les embryons destinés à Live-imagerie utilisant le protocole goutte suspendue

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Introduction

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Step 1: Collect Embryos

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Step 2: Prepare a Dechorionation Slide

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Step 3: Prepare Imaging Chamber

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Step 4: Place Halocarbon Oil on a Coverslip

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Step 5: Picking Embryos From Plates

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Step 6: Place Embryos onto Tape

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Step 7: Roll Embryos to Remove the Chorion

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Step 8: Transfer Embryos to Halocarbon Oil

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Step 9: Push Embryos to Bottom of Oil

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Step 10: Invert Coverslip on Live Imaging Chamber

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Step 11: Secure Coverslip with Tape