Name: cancer borealis stomatogastric nervous system dissection
Uploaded: 2009-03-23T00:00:00
Description: Watch this Scientific Journal Video about Cancer Borealis Stomatogastric Nervous System Dissection at JoVE.com

我们非常感谢黄鼠狼实验室成员。我们感谢她的支持和鼓励我们的顾问，除夕黄鼠狼，博士。这项研究是由美国国家神经疾病研究所和NS - 17813和NS - 058110中风资助支持。

1。毛夹层<ol><li>在冰上放置30分钟沉闷的动物的感觉和运动系统的螃蟹。用冰各方环绕它。处理蟹时，必须戴上手套。 </li><li>总值剥离准备铺设夹层锅，您将使用的工具。 Rongeurs，骨切割剪刀，小剪刀，有齿镊，锅铲锥形边，一个黑色的Sylgard涂层盘，和昆虫引脚是必要的。 </li><li>删除其后腿的螃蟹从冰桶，以防止捏它和它放置在夹层锅。 </li><li>删除先扭每一个外来的爪子。然后取出使用相同?...

<ol>
	<li>Harris-Warrick, R. M., Marder, E., Selverston, A. I., Moulins, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Dynamic Biological Networks: The stomatogastric nervous system. , (1992).</li><li>Marder, E., Bucher, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Understanding+circuit+dynamics+using+the+stomatogastric+nervous+system+of+lobsters+and+crabs.">Understanding circuit dynamics using the stomatogastric nervous system of lobsters and crabs.</a> Annu. Rev. Physiol. 69, 291-316 (2007).</li><li>Maynard, D. M., Dando, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+structure+of+the+stomatogastric+neuromuscular+system+in+Callinectes+sapidus,+Homarus+Americanus+and+Panulirus+argus+(Decapoda+crustacean).">The structure of the stomatogastric neuromuscular system in Callinectes sapidus, Homarus Americanus and Panulirus argus (Decapoda crustacean).</a> Philos. Trans. R. Soc. Lond. B Biol. Sci. 268 (892), 161-220 (1974).</li><li>Selverston, A. I., Russell, D. F., Miller, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Stomatogastric+Nervous+System:+Structure+and+function+of+a+small+neural+network.">The Stomatogastric Nervous System: Structure and function of a small neural network.</a> Prog. Neurobiology. 7, 215-290 (1976).</li></ol>

STNS清扫是在执行一个电或免疫组织化学实验，或获得细胞培养细胞的第一步。不管预定的实验，总值剥离将维持不变。然而，优良的剥离可能会有所不同。重要的是要未雨绸缪的时间，你将记录从细胞和其中的神经。在STG的大多数神经元支配在肚子里至少有一个肌肉（见图2），并分类根据自己的支配目标的进程。因此，外录音需要充分验证的一个细胞内正在录制的细胞的身份。幽门节奏的频率?...

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		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Black Sylgard 170</td>
<td>Other</td>
<td>Dow Corning</td>
<td>170</td>
<td>Gross Dissection</td>
</tr>
<tr>
<td>Fine Scissors</td>
<td>Surgery</td>
<td>FST</td>
<td>14090-09</td>
<td>&nbsp;</td>
<tr>
<td>Insect Pins size 6</td>
<td>Surgery</td>
<td>FST</td>
<td>26000-65</td>
<td>&nbsp;</td>
<tr>
<td>Mayo Scissors</td>
<td>Surgery</td>
<td>FST</td>
<td>14110-17</td>
<td>&nbsp;</td>
<tr>
<td>Micro-Spatula</td>
<td>Surgery</td>
<td>Ward Sci.</td>
<td>15 V 4313</td>
<td>&nbsp;</td>
<tr>
<td>Rongeurs</td>
<td>Surgery</td>
<td>FST</td>
<td>16000-14</td>
<td>&nbsp;</td>
<tr>
<td>Tissue Forceps</td>
<td>Surgery</td>
<td>FST</td>
<td>11021-12</td>
<td>&nbsp;</td>
<tr>
<td>Fine Tungsten Wire (0.001 in. diameter)</td>
<td>Surgery</td>
<td>California Fine Wire</td>
<td>&nbsp;</td>
<td>Fine Dissection</td>
</tr>
<tr>
<td>Forceps #5</td>
<td>Surgery</td>
<td>FST</td>
<td>91150-20</td>
<td>&nbsp;</td>
<tr>
<td>Minuten Pins</td>
<td>Surgery</td>
<td>FST</td>
<td>26002-10</td>
<td>&nbsp;</td>
<tr>
<td>Moria Spring Scissors (7mm blades)</td>
<td>Surgery</td>
<td>FST</td>
<td>15370-52</td>
<td>&nbsp;</td>
<tr>
<td>Petri Dish (100x15mm)</td>
<td>Other</td>
<td>Fischer	</td>
<td>08-757-12</td>
<td>&nbsp;</td>
<tr>
<td>Pin Holder</td>
<td>Surgery</td>
<td>FST</td>
<td>26018-17</td>
<td>&nbsp;</td>
<tr>
<td>Super fine forceps #5SF</td>
<td>Surgery</td>
<td>FST</td>
<td>11252-00</td>
<td>&nbsp;</td>
<tr>
<td>Sylgard 182</td>
<td>Other</td>
<td>Dow Corning</td>
<td>182</td>
<td>&nbsp;</td>
<tr>
<td>Tungsten Needles</td>
<td>Surgery</td>
<td>FST</td>
<td>10130-05</td>
<td>&nbsp;</td>
<tr>
<td>Dissecting Microscope</td>
<td>Microscope</td>
<td>Wild</td>
<td>MC3</td>
<td>Misc.</td>
</tr>
<tr>
<td>Jonah Crab (C. borealis)</td>
<td>Animal</td>
<td>Commercial Lobster</td>
<td>&nbsp;</td>
<td>&nbsp;</td>		</tbody>
		</table>
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cancer borealis stomatogastric nervous system dissection

stomatogastric节（STG）是一个很好的模型研究细胞和网络交互，因为它包含一个细胞的数量相对较少（ 约25 C。北欧化工）这是很好的特点。在STG的细胞表现出广泛的产出和胃电机行动负责。胃中含有打破了三个内部的牙齿，食物和过滤的食物，才达到的肠幽门胃磨。 STG产生两个节奏的输出来控制称为中枢模式发生器（CPGs）胃磨和幽门。 STG在每个单元格可以参加一个或这两种节奏。这些CPGs允许研究的神经调节，动态平衡，变异细胞和网络，网络的发展，和网络恢复。 解剖的stomatogastric从约拿蟹（ 癌症北欧化工 ）（STNS）神经系统两部分;总值和罚款的夹层。总值剥离整个胃解剖螃蟹。在精细剥离STNS提取使用解剖镜下微解剖工具（见图1）胃。 STNS包括STG，食道神经节（OG），并连合神经节（COG）以及腹部肌肉的神经支配。在这里，我们显示如何执行准备在STG的细胞，将细胞内和周围神经记录电生理实​​验STNS完成清扫，将用于外录音。显示我们的desheathing揭示somata和神经纤维的神经节技术，以及适当的技术，为寻找所需的的神经。

Watch this Scientific Journal Video about Cancer Borealis Stomatogastric Nervous System Dissection at JoVE.com

Cancer Borealis Stomatogastric Nervous System Dissection

stomatogastric神经系统约拿蟹（STNS）（<em&gt; C。北欧化工公司</em&gt;）可用于电生理，免疫组织化学和细胞培养的研究。 STNS提取两部分：总值和罚款的夹层。

癌症北欧化工 Stomatogastric神经系统解剖

The stomatogastric ganglion (STG) is an excellent model for studying cellular and network interactions because it contains a relatively small number of ...

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Biology

Cancer Borealis Stomatogastric Nervous System Dissection

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