Name: gene-gun transfection of hippocampal neurons
Uploaded: 2006-11-30T00:00:00
Description: Watch this Scientific Journal Video about Gene-gun Transfection of Hippocampal Neurons at JoVE.com

gene-gun transfection of hippocampal neurons

Watch this Scientific Journal Video about Gene-gun Transfection of Hippocampal Neurons at JoVE.com

Gene-gun Transfection of Hippocampal Neurons

Gene-gun Transfektion von Neuronen im Hippocampus

Research

JoVE Journal

Biology

Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells

Produktion von replikationsdefekte Retrovirus durch transiente Transfektion von 293T-Zellen

Our lab studies human myeloproliferative diseases induced by such oncogenes as Bcr-Abl or growth factor receptor-derived oncogenes (ZNF198-FGFR1, ...

Forschung

Biologie

Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture

Vorbereitung der Gene Gun und Aufzählungszeichen Biolistic Transfektion von Neuronen in Scheiben Kultur

Biolistic transfection is a physical means of transfecting cells by bombarding tissue with high velocity DNA coated particles. We provide a detailed ...

Primary Culture of Hippocampal Neurons from P0 Newborn Rats

Primäre Kultur des Hippocampus-Neuronen von neugeborenen Ratten P0

The physiological properties of hippocampal neurons are commonly investigated, especially because of the involvement of the hippocampus in learning and ...

Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons

Live-Imaging von Dense-Core-Vesikel in Primary hippokampalen Neuronen

Observing and characterizing dynamic cellular processes can yield important information about cellular activity that cannot be gained from static images.  ...

DNA Transfection of Mammalian Skeletal Muscles using <em>In Vivo</em> Electroporation

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

DNA Transfektion von Säuger Skelettmuskulatur mit<em> In Vivo</em> Elektroporation

 A growing interest in cell biology is to express transgenically modified forms of essential proteins (e.g. fluorescently tagged constructs and/or mutant ...

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Dissection der hippocampalen von erwachsenen Maus

The hippocampus is one of the most widely studied areas in the brain because of its important functional role in memory processing and learning, its ...

Time-lapse Imaging of Mitosis After siRNA Transfection

Zeitraffer-Imaging der Mitose Nach siRNA-Transfektion

Changes in cellular organization and chromosome dynamics that occur during mitosis are tightly coordinated to ensure accurate inheritance of genomic and ...

Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

Bimolecular Fluorescence Complementation BiFC Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

Bimolekularen Fluoreszenz Komplementation (BIFC) Assay für Protein-Protein-Wechselwirkungen in Onion Zellen unter Verwendung des Helios Gene Gun

Investigation of gene function in diverse organisms relies on knowledge of how the gene products interact with each other in their normal cellular ...

Single Cell Transfection in Chick Embryos

Single Cell Transfektion in Hühnerembryonen

A central theme in developmental biology is the diversification of lineages and the elucidation of underlying molecular mechanisms. This entails a ...

Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons

Effiziente Differenzierung von embryonalen Stammzellen der Maus in Motoneuronen

Direct differentiation of embryonic stem (ES) cells into functional  motor neurons represents a promising resource to study disease mechanisms, to  screen ...