Name: dissecting the non-human primate brain in stereotaxic space
Uploaded: 2009-07-16T20:00:00
Description: Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

作者想感谢他持续的技术支持Ikiel Ptito。 NSERC授予到MP。

第1部分：前处理的组织<ol><li>组织应多聚甲醛，戊二醛，或福尔马林灌注。这可以通过标准transcardial，通常用于收获其他器官灌注。在本研究的主题是深刻的盐酸氯胺酮（10毫克/公斤，IM）与戊巴比妥钠（25毫克/公斤，四）过量安乐死，镇静剂和灌注用0​​.1 M PBS transcardially其次，直到完全exsanguinated.This在PBS的4％多聚甲醛为5分钟（约1升）的解决方案。 </li></ol>第2部分：立体定?...

<ol>
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圣基茨vervet（Chlorocebus aethiops sabeus）是旧世界的灵长类动物与人类类似的模式和皮层和皮层下的大脑发育率。该物种已被用来模拟复杂的人类行为障碍，如焦虑行为8，大脑半球切除术，高血压，帕金森氏病10 Alzhemier 的疾病11， 和酗酒12。最近，该物种已被用于研究自然产前乙醇暴露的神经解剖学的影响。怀孕vervets被允许在孕晚期相当于3-5每周四次的标准饮...

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dissecting the non-human primate brain in stereotaxic space

使用非人类灵长类动物在人类发育和衰老过程的认识提供了一个优秀的翻译模式<sup&gt; 1-6</sup&gt;。此外，使用非人类灵长类动物最近提供的机会，自然模型复杂的精神疾病，如酗酒<sup&gt; 7</sup&gt;。在这里，我们描述了一种阻断冠状面vervet猴子大脑的技术（<em&gt; Chlorocebus aethiops sabeus</em&gt;）在立体空间中的完整的头骨。这里介绍的方法提供了一个标准节块之间和科目和块之间的最小化的部分路段平面。切片的组织块在冠状面，也有利于感兴趣的领域划分。因为单个半球的vervet猴子产量1200多节，在50μm的切片时，这种方法提供了管理大小的块。此外，通过阻断大脑切成1cm块，它有利于普及率为cyroprotection蔗糖，并允许在一个标准的低温​​恒温器切片块。

Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

Dissecting the Non-human Primate Brain in Stereotaxic Space

非人类灵长类动物是一种重要的翻译物种为我们理解大脑的正常处理。在人体正常和病理条件下的灵长类动物的大脑解剖组织可以提供重要的见解。

剖析在非人类灵长类动物脑立体定向空间

The use of non-human primates provides an excellent translational model for our understanding of developmental and aging processes in humans1-6. In ...

Research

JoVE Journal

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Survivable Stereotaxic Surgery in Rodents 

在啮齿类动物的存活立体定向手术

The ability to measure extracellular basal levels of neurotransmitters in the brain of awake animals allows for the determination of effects of different ...

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Dissecting and Recording from The C. Elegans Neuromuscular Junction

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Neurotransmission is the process by which neurons transfer information via chemical signals to their post-synaptic targets, on a rapid time scale.   This ...

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到大脑的门户：剖析灵长类动物的眼睛

The visual system in humans is considered the  gateway  to the world and plays a principal role in the plethora of sensory, perceptual and cognitive ...

Knowing What Counts: Unbiased Stereology in the Non-human Primate Brain

知道重要的：无偏体视学在非人类灵长类动物的脑

The non-human primate is an important translational species for understanding the normal function and disease processes of the human brain. Unbiased ...

Trajectory Data Analyses for Pedestrian Space-time Activity Study

轨迹数据分析行人时空活性研究

It is well recognized that human movement in the  spatial and temporal dimensions has direct influence on disease transmission1-3.  An infectious disease ...

Sampling Blood from the Lateral Tail Vein of the Rat

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Blood samples are commonly obtained in many experimental contexts to measure targets of interest, including hormones, immune factors, growth factors, ...

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Renal micropuncture and renal 2-photon imaging are seminal techniques in renal physiology. However, micropuncture is limited by dependence on conventional ...

Reverse Dissection and DiceCT Reveal Otherwise Hidden Data in the Evolution of the Primate Face

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Facial expressions, or facial displays, of social or emotional intent are produced by many mammalian taxa as a means of visually communicating with ...

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

利用两色单分子荧光在原位杂交中Escherichia coli探索大肠杆菌中空间和时间中的 mRNA 动力学

Single-molecule fluorescence in situ hybridization (smFISH) allows for counting the absolute number of mRNAs in individual cells. Here, we describe an ...

Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy

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Ultrafast force-clamp spectroscopy (UFFCS) is a single molecule technique based on laser tweezers that allows the investigation of the chemomechanics of ...