Name: dissecting the non-human primate brain in stereotaxic space
Uploaded: 2009-07-16T20:00:00
Description: Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

Les auteurs tiennent à remercier pour son Ikiel Ptito appui technique continu. Subvention du CRSNG au MP.

Partie 1: Pré-traitement des tissus <ol><li> Les tissus doivent être bien perfusés avec du paraformaldéhyde, glutaraldéhyde, ou du formol. Ceci peut être réalisé grâce à la perfusion transcardial norme généralement utilisée pour la récolte d&#39;autres organes. Dans l&#39;étude actuelle, l&#39;objet a été profondément sédatés avec le chlorhydrate de kétamine (10 mg / kg, im), euthanasiés avec une overdose de pentobarbital sodique (25 mg / kg, iv) et perfusé transcardiaque avec PBS 0,1 M jusqu&#3...

<ol>
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Le Saint-Kitts-vervet (aethiops Chlorocebus sabeus) est un primate de l&#39;Ancien Monde avec des motifs similaires et les taux de développement du cerveau cortical et sous-corticales à celui des humains. Cette espèce a été utilisé pour modéliser les troubles du comportement humain complexe comme un comportement anxieux, hypertension artérielle 8, 9 hémisphérectomie, la maladie de Parkinson 10, la maladie Alzhemier de 11, et l&#39;abus d&#39;alcool 12. Plus ...

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<td>Scalpel</td>
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<td>Any surgical scissors are sufficient</td>
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dissecting the non-human primate brain in stereotaxic space

L&#39;utilisation de primates non-humains fournit un excellent modèle de translation pour notre compréhension des processus de développement et le vieillissement chez les humains<sup&gt; 1-6</sup&gt;. En outre, l&#39;utilisation de primates non humains a récemment eu l&#39;occasion d&#39;un modèle complexe naturellement troubles psychiatriques tels que l&#39;abus d&#39;alcool<sup&gt; 7</sup&gt;. Nous décrivons ici une technique pour bloquer le cerveau dans le plan frontal du singe vervet (<em&gt; Chlorocebus aethiops sabeus</em&gt;) Dans le crâne intact dans l&#39;espace stéréotaxique. La méthode décrite ici fournit un avion standard de la section entre les blocs et les sujets et minimise coupes partielles entre les blocs. Sectionnement d&#39;un bloc de tissu dans le plan frontal facilite également la délimitation d&#39;une zone d&#39;intérêt. Cette méthode fournit gérable blocs de taille car un seul hémisphère de l&#39;singe vervet rendements de plus de 1200 sections quand le tranchage à 50 microns. Par ailleurs, en bloquant le cerveau en blocs de 1 cm, elle facilite la pénétration du saccharose pour cyroprotection et permet au bloc d&#39;être tranché sur un cryostat standard.

Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

Dissecting the Non-human Primate Brain in Stereotaxic Space

Le primate non-humain est une espèce importante pour notre compréhension de translation du traitement normal du cerveau. L&#39;organisation anatomique du cerveau des primates peuvent fournir des indications importantes sur les conditions normales et pathologiques chez l&#39;homme.

Disséquer le cerveau des primates non humains dans l&#39;espace stéréotaxique

The use of non-human primates provides an excellent translational model for our understanding of developmental and aging processes in humans1-6. In ...

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