Name: dissecting the non-human primate brain in stereotaxic space
Uploaded: 2009-07-16T20:00:00
Description: Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

Die Autoren bedanken sich bei Ikiel Ptito für seine weitere fachliche Unterstützung danken. NSERC Zuschuss zu MP.

Teil 1: Pre-Processing von Gewebe <ol><li> Tissue sollte gut mit Paraformaldehyd, Glutaraldehyd oder Formalin perfundiert werden. Dies kann durch Standard-transcardial Perfusion in der Regel verwendet, um andere Organe Ernte erreicht werden. In der vorliegenden Studie das Thema war tief mit Ketaminhydrochlorid (10 mg / kg, im), mit einer Überdosis Natrium-Pentobarbital (25 mg / kg, iv) eingeschläfert sediert und perfundierten transkardial mit 0,1 M PBS, bis sie vollständig exsanguinated.This folgt von einem 4% Para...

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	<li>Gallagher, M., Rapp, P. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+animal+models+to+study+the+effects+of+aging+on+cognition.+Annu+Rev+Psychol+48.">The use of animal models to study the effects of aging on cognition. Annu Rev Psychol 48.</a> , 339-370 (1997).</li><li>Clancy, B., Darlington, R., Finlay, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Translating+developmental+time+across+mammalian+species.">Translating developmental time across mammalian species.</a> Neuroscience. 105, 7-17 (2001).</li><li>Nowakowski, R. 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N., Guzman-Flores, C., Juarez, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Voluntary+consumption+of+beverage+alcohol+by+vervet+monkeys:+population+screening,+descriptive+behavior+and+biochemical+measures.">Voluntary consumption of beverage alcohol by vervet monkeys: population screening, descriptive behavior and biochemical measures.</a> Pharmacol Biochem Behav. 36, 367-373 (1990).</li><li>Palmour, R. M., Mulligan, J., Howbert, J. 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Die St. Kitts vervet (Chlorocebus aethiops sabeus) ist ein Old World Primaten mit ähnlichen Mustern und Preisen von kortikalen und subkortikalen die Entwicklung des Gehirns wie das des Menschen. Diese Spezies wurde verwendet, um komplexe menschliche Verhaltensstörungen wie ängstliches Verhalten, Bluthochdruck 8, Hemisphärektomie 9, Parkinson-Krankheit 10, Alzhemier-Krankheit 11, und Alkoholmissbrauch 12 Modell. In jüngerer Zeit hat diese Art verwendet ...

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<td>Scalpel</td>
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<td>Fine Science Tools</td>
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<td>Any surgical scissors are sufficient</td>
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dissecting the non-human primate brain in stereotaxic space

Die Verwendung von nicht-menschlichen Primaten bietet eine hervorragende translationale Modell für unser Verständnis der Entwicklungs-und Alterungsprozessen beim Menschen<sup&gt; 1-6</sup&gt;. Darüber hinaus hat die Verwendung von nicht-menschlichen Primaten vor kurzem die Gelegenheit geboten, natürlich Modellierung komplexer psychiatrischer Störungen wie Alkoholmissbrauch<sup&gt; 7</sup&gt;. Hier beschreiben wir eine Technik für die Sperrung des Gehirns in der koronalen Ebene der grünen Meerkatze (<em&gt; Chlorocebus aethiops sabeus</em&gt;) In die intakte Schädeldecke in stereotaktischen Raum. Die hier beschriebene Methode bietet einen Standard Schnittfläche zwischen den Blöcken und Themen und minimiert Teilstrecken zwischen den Blöcken. Schnitte eines Blocks von Gewebe in der koronalen Ebene erleichtert auch die Abgrenzung eines Gebiets von Interesse. Diese Methode bietet überschaubar großen Blöcken, da eine einzelne Hemisphäre des grünen Meerkatze liefert mehr als 1200 Teile beim Schneiden bei 50 um. Außerdem blockiert das Gehirn in 1 cm-Blöcke, erleichtert es das Eindringen von Saccharose cyroprotection und ermöglicht den Block auf einem Standard-Kryostat geschnitten werden.

Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

Dissecting the Non-human Primate Brain in Stereotaxic Space

Die nicht-menschlichen Primaten ist ein wichtiger translationale Arten für unser Verständnis der normalen Verarbeitung des Gehirns. Die anatomische Organisation des Primas Gehirn kann wichtige Einblicke in normalen und pathologischen Bedingungen beim Menschen liefern.

Grundlagen zur nicht-menschlichen Primaten Brain in stereotaktischen Raum

The use of non-human primates provides an excellent translational model for our understanding of developmental and aging processes in humans1-6. In ...

Research

JoVE Journal

Biology

Survivable Stereotaxic Surgery in Rodents 

Survivable stereotaktischen Chirurgie in Nagetiere

The ability to measure extracellular basal levels of neurotransmitters in the brain of awake animals allows for the determination of effects of different ...

Forschung

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Dissecting and Recording from The C. Elegans Neuromuscular Junction

Dissecting und Aufnahme von C. elegans neuromuskulären Synapse

Neurotransmission is the process by which neurons transfer information via chemical signals to their post-synaptic targets, on a rapid time scale.   This ...

The Gateway to the Brain: Dissecting the Primate Eye

Das Tor zum Gehirn: Die Biologie des Primas Eye

The visual system in humans is considered the  gateway  to the world and plays a principal role in the plethora of sensory, perceptual and cognitive ...

Knowing What Counts: Unbiased Stereology in the Non-human Primate Brain

Zu wissen, was zählt: Unbiased Stereology in der nicht-menschlichen Primaten Gehirn

The non-human primate is an important translational species for understanding the normal function and disease processes of the human brain. Unbiased ...

Trajectory Data Analyses for Pedestrian Space-time Activity Study

Bahndaten Analysen für Fußgänger Raum-Zeit-Activity Study

It is well recognized that human movement in the  spatial and temporal dimensions has direct influence on disease transmission1-3.  An infectious disease ...

Sampling Blood from the Lateral Tail Vein of the Rat

Blutprobenentnahme aus der laterale Schwanzvene der Ratte

Blood samples are commonly obtained in many experimental contexts to measure targets of interest, including hormones, immune factors, growth factors, ...

Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy

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Renal micropuncture and renal 2-photon imaging are seminal techniques in renal physiology. However, micropuncture is limited by dependence on conventional ...

Reverse Dissection and DiceCT Reveal Otherwise Hidden Data in the Evolution of the Primate Face

Reverse-Dissektion und DiceCT offenbaren sonst versteckt Daten in der Evolution der Primaten-Gesicht

Facial expressions, or facial displays, of social or emotional intent are produced by many mammalian taxa as a means of visually communicating with ...

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Probing mRNA Kinetics in Space and Time in Escherichia coli mit Zwei-Farben-Einmolekülfluoreszenz in Situ-Hybridisierung

Single-molecule fluorescence in situ hybridization (smFISH) allows for counting the absolute number of mRNAs in individual cells. Here, we describe an ...

Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy

Analyse der mechanoenzymatischen Eigenschaften prozessiver Myosine mit ultraschneller Kraftklemmenspektroskopie

Ultrafast force-clamp spectroscopy (UFFCS) is a single molecule technique based on laser tweezers that allows the investigation of the chemomechanics of ...