Name: dissecting the non-human primate brain in stereotaxic space
Uploaded: 2009-07-16T20:00:00
Description: Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

Gli autori desiderano ringraziare Ikiel Ptito per il suo continuo supporto tecnico. NSERC concedere a MP.

Parte 1: Pre-trattamento dei tessuti <ol><li> Tessuto deve essere ben perfusi con paraformaldeide, glutaraldeide, o formalina. Questo può essere raggiunto attraverso standard di perfusione transcardial tipicamente usato per raccogliere altri organi. Nel presente studio il soggetto era profondamente sedato con cloridrato di ketamina (10 mg / kg, im), eutanasia con una dose eccessiva di sodio pentobarbital (25 mg / kg, iv) e perfusi transcardially con 0,1 M PBS fino a completo exsanguinated.This è seguito da una soluz...

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	<li>Gallagher, M., Rapp, P. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+animal+models+to+study+the+effects+of+aging+on+cognition.+Annu+Rev+Psychol+48.">The use of animal models to study the effects of aging on cognition. Annu Rev Psychol 48.</a> , 339-370 (1997).</li><li>Clancy, B., Darlington, R., Finlay, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Translating+developmental+time+across+mammalian+species.">Translating developmental time across mammalian species.</a> Neuroscience. 105, 7-17 (2001).</li><li>Nowakowski, R. 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N., Guzman-Flores, C., Juarez, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Voluntary+consumption+of+beverage+alcohol+by+vervet+monkeys:+population+screening,+descriptive+behavior+and+biochemical+measures.">Voluntary consumption of beverage alcohol by vervet monkeys: population screening, descriptive behavior and biochemical measures.</a> Pharmacol Biochem Behav. 36, 367-373 (1990).</li><li>Palmour, R. M., Mulligan, J., Howbert, J. 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Il St. Kitts cercopiteco (Chlorocebus aethiops sabeus) è un primate del Vecchio Mondo, con modelli simili e tassi di sviluppo del cervello corticali e sottocorticali a quella degli esseri umani. Questa specie è stata usata per modellare complessi disturbi del comportamento umano come comportamento ansioso, ipertensione 8, emisferectomia 9, 10 morbo di Parkinson, il morbo di Alzhemier il 11 e l&#39;abuso di alcool 12. Più recentemente, questa specie è stata utilizz...

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dissecting the non-human primate brain in stereotaxic space

L&#39;utilizzo di primati non umani fornisce un modello eccellente traslazionale per la nostra comprensione dei processi di sviluppo e di invecchiamento negli esseri umani<sup&gt; 1-6</sup&gt;. Inoltre, l&#39;uso di primati non umani ha recentemente dato l&#39;opportunità di modellare naturalmente complessi disturbi psichiatrici, come l&#39;abuso di alcool<sup&gt; 7</sup&gt;. Qui si descrive una tecnica per bloccare il cervello nel piano coronale della scimmia cercopiteco (<em&gt; Chlorocebus aethiops sabeus</em&gt;) Nel cranio intatto nello spazio stereotassico. Il metodo descritto fornisce: ecco un piano standard di sezione tra blocchi e soggetti e minimizza le sezioni parziali tra i blocchi. Sezionando un blocco di tessuto sul piano coronale facilita anche la definizione di una zona di interesse. Questo metodo fornisce blocchi di dimensioni gestibili in quanto un singolo emisfero della scimmia cercopiteco produce più di 1200 parti quando taglio a 50 micron. Inoltre, bloccando il cervello in blocchi 1 cm, che facilita la penetrazione di saccarosio per cyroprotection e permette il blocco da affettare su un criostato standard.

Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

Dissecting the Non-human Primate Brain in Stereotaxic Space

Il primate non umano è una specie importante traslazionale per la nostra comprensione della normale elaborazione del cervello. L&#39;organizzazione anatomica del cervello dei primati in grado di fornire spunti importanti in condizioni normali e patologiche nell&#39;uomo.

Dissezione di non-umano cervello dei primati nello spazio Stereotassica

The use of non-human primates provides an excellent translational model for our understanding of developmental and aging processes in humans1-6. In ...

Research

JoVE Journal

Biology

Survivable Stereotaxic Surgery in Rodents 

Survivable Chirurgia Stereotassica in roditori

The ability to measure extracellular basal levels of neurotransmitters in the brain of awake animals allows for the determination of effects of different ...

Ricerca

Biologia

Dissecting and Recording from The C. Elegans Neuromuscular Junction

Dissezione e registrazione dal bivio C. Elegans neuromuscolare

Neurotransmission is the process by which neurons transfer information via chemical signals to their post-synaptic targets, on a rapid time scale.   This ...

The Gateway to the Brain: Dissecting the Primate Eye

La Porta del Cervello: Dissezione l&#39;Occhio Primate

The visual system in humans is considered the  gateway  to the world and plays a principal role in the plethora of sensory, perceptual and cognitive ...

Knowing What Counts: Unbiased Stereology in the Non-human Primate Brain

Conoscere ciò che conta: stereologia Tutti nel settore non umano cervello dei primati

The non-human primate is an important translational species for understanding the normal function and disease processes of the human brain. Unbiased ...

Trajectory Data Analyses for Pedestrian Space-time Activity Study

Le analisi per i dati di traiettoria pedonale Lo spazio-tempo di attività di studio

It is well recognized that human movement in the  spatial and temporal dimensions has direct influence on disease transmission1-3.  An infectious disease ...

Sampling Blood from the Lateral Tail Vein of the Rat

Prelievo di sangue dalla vena della coda laterale del Topo

Blood samples are commonly obtained in many experimental contexts to measure targets of interest, including hormones, immune factors, growth factors, ...

Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy

Micropuntura dello spazio di Bowman in topi nei paraggi 2 Photon microscopia

Renal micropuncture and renal 2-photon imaging are seminal techniques in renal physiology. However, micropuncture is limited by dependence on conventional ...

Reverse Dissection and DiceCT Reveal Otherwise Hidden Data in the Evolution of the Primate Face

Invertire la dissezione e dati DiceCT Reveal altrimenti nascosti nell'evoluzione del Primate viso

Facial expressions, or facial displays, of social or emotional intent are produced by many mammalian taxa as a means of visually communicating with ...

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Sondare la cinetica dell'mRNA nello spazio e nel tempo in Escherichia coli utilizzando la fluorescenza a singola molecola a due colori in ibridazione situ

Single-molecule fluorescence in situ hybridization (smFISH) allows for counting the absolute number of mRNAs in individual cells. Here, we describe an ...

Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy

Dissezione delle proprietà meccanoenzimatiche delle miosine processive con spettroscopia ultraveloce a force-clamp

Ultrafast force-clamp spectroscopy (UFFCS) is a single molecule technique based on laser tweezers that allows the investigation of the chemomechanics of ...