Name: dissecting the non-human primate brain in stereotaxic space
Uploaded: 2009-07-16T20:00:00
Description: Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

著者は、彼の継続的な技術サポートのためにIkiel Ptitoに感謝します。 MPにNSERC助成金。

パート1：組織の前処理<ol><li>組織はよくパラホルムアルデヒド、グルタルアルデヒド、またはホルマリンで灌流してください。これは通常、他の臓器を収穫するために使用される標準transcardial灌流によって達成することができます。完全にexsanguinated.Thisが続いされるまで本研究では被験者は深く、（10 mg / kgを、IM）塩酸ケタミンで鎮静したペントバルビタールナトリウムの過剰投与（25 mg / ...

<ol>
	<li>Gallagher, M., Rapp, P. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+animal+models+to+study+the+effects+of+aging+on+cognition.+Annu+Rev+Psychol+48.">The use of animal models to study the effects of aging on cognition. Annu Rev Psychol 48.</a> , 339-370 (1997).</li><li>Clancy, B., Darlington, R., Finlay, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Translating+developmental+time+across+mammalian+species.">Translating developmental time across mammalian species.</a> Neuroscience. 105, 7-17 (2001).</li><li>Nowakowski, R. 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セントキッツベルベットモンキー（Chlorocebus aethiops sabeusは ）人間のように皮質と皮質下の脳の発達の類似したパターンと速度と旧世界霊長類です。この種は、不安な動作のような複雑な人間の行動障害、高血圧症8、大脳半球切除術9、パーキンソン病10、Alzhemier病11、およびアルコールの乱用12をモデル化するために使用されています。さらに最...

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		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>Scalpel</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>10003-12</td>
<td>&nbsp;</td>
<tr>
<td>Scalpel blades</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>10011-00</td>
<td>&nbsp;</td>
<tr>
<td>Scissors</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>14090-11</td>
<td>Any surgical scissors are sufficient</td>
</tr>
<tr>
<td>Rongeurs</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>16121-14</td>
<td>&nbsp;</td>
<tr>
<td>Forceps</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>11027-12</td>
<td>&nbsp;</td>
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<td>Filter paper</td>
<td>&nbsp;</td>
<td>Fisher</td>
<td>09-924-150</td>
<td>&nbsp;</td>
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<td>Stereotaxic Frame</td>
<td>&nbsp;</td>
<td>Kopf Instruments</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>Tissue slicing blade</td>
<td>&nbsp;</td>
<td>Thomas</td>
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dissecting the non-human primate brain in stereotaxic space

ヒト以外の霊長類の使用は、ヒトの発達と老化の理解のための優秀な翻訳モデルを提供<sup&gt; 1月6日</sup&gt;。さらに、ヒト以外の霊長類の使用は最近、自然にそのようなアルコールの乱用などの複雑な精神疾患をモデル化する機会を与えている<sup&gt; 7</sup&gt;。ここでは、ベルベットモンキーの冠状面で脳をブロックするための手法を記述する（<em&gt; Chlorocebus aethiops sabeus</em&gt;）定位空間にそのまま頭蓋骨インチここで説明する方法はブロックと対象とブロックの間を最小限に抑えるの部分のセクションの間にセクションの標準的な平面を提供します。冠状面での組織のブロックの切削することも関心の領域の線引きを容易にします。 50μmの時にスライスするときにベルベットモンキーの単一の半球は、1200以上のセクションをもたらすので、このメソッドは、管理可能なサイズのブロックを提供します。さらに1センチメートルブロックに脳をブロックすることによって、それはcyroprotectionためにショ糖の浸透を容易にし、ブロックが標準的なクライオスタットにスライスすることができます。

Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

Dissecting the Non-human Primate Brain in Stereotaxic Space

ヒト以外の霊長類は脳の正常な処理の理解のための重要な並進種です。霊長類の脳の解剖学的組織は、ヒトの正常と病理学的状態に重要な洞察を提供することができます。

定位空間における非ヒト霊長類の脳を解剖

The use of non-human primates provides an excellent translational model for our understanding of developmental and aging processes in humans1-6. In ...

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Biology

Survivable Stereotaxic Surgery in Rodents 

げっ歯類で存続可能な定位手術

The ability to measure extracellular basal levels of neurotransmitters in the brain of awake animals allows for the determination of effects of different ...

Dissecting and Recording from The C. Elegans Neuromuscular Junction

解離性とC. elegansの神経筋接合部から録音する

Neurotransmission is the process by which neurons transfer information via chemical signals to their post-synaptic targets, on a rapid time scale.   This ...

The Gateway to the Brain: Dissecting the Primate Eye

脳へのゲートウェイ：霊長目の解剖

The visual system in humans is considered the  gateway  to the world and plays a principal role in the plethora of sensory, perceptual and cognitive ...

Knowing What Counts: Unbiased Stereology in the Non-human Primate Brain

非ヒト霊長類の脳の公平な立体学を：大切なのを知ること

The non-human primate is an important translational species for understanding the normal function and disease processes of the human brain. Unbiased ...

Trajectory Data Analyses for Pedestrian Space-time Activity Study

歩行者空間·時間·アクティビティ·研究のための軌道データ解析

It is well recognized that human movement in the  spatial and temporal dimensions has direct influence on disease transmission1-3.  An infectious disease ...

Sampling Blood from the Lateral Tail Vein of the Rat

ラットの外側尾静脈から血液をサンプリング

Blood samples are commonly obtained in many experimental contexts to measure targets of interest, including hormones, immune factors, growth factors, ...

Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy

2 光子顕微鏡による促進マウス Bowman 腔のそれによって

Renal micropuncture and renal 2-photon imaging are seminal techniques in renal physiology. However, micropuncture is limited by dependence on conventional ...

Reverse Dissection and DiceCT Reveal Otherwise Hidden Data in the Evolution of the Primate Face

郭清と霊長類の顔の進化における DiceCT 明らかにする別の方法で非表示のデータを逆に

Facial expressions, or facial displays, of social or emotional intent are produced by many mammalian taxa as a means of visually communicating with ...

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Situハイブリダイゼーションにおける2色単分子蛍光を用いた 大腸菌 における空間と時間におけるmRNA運動学の探査

Single-molecule fluorescence in situ hybridization (smFISH) allows for counting the absolute number of mRNAs in individual cells. Here, we describe an ...

Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy

超高速フォースクランプ分光法によるミオシンのメカノ酵素的性質の解剖

Ultrafast force-clamp spectroscopy (UFFCS) is a single molecule technique based on laser tweezers that allows the investigation of the chemomechanics of ...