Name: dissecting the non-human primate brain in stereotaxic space
Uploaded: 2009-07-16T20:00:00
Description: Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

Os autores gostariam de agradecer Ikiel Ptito por seu contínuo apoio técnico. NSERC conceder ao MP.

Parte 1: Pré-processamento de tecido <ol><li> Tecido deve ser bem perfundidos com paraformaldeído, glutaraldeído ou formalina. Isto pode ser conseguido através de perfusão transcardial padrão normalmente utilizado para a colheita de outros órgãos. No presente estudo o assunto estava profundamente sedado com cloridrato de cetamina (10 mg / kg, im), sacrificados com uma overdose de pentobarbital sódico (25 mg / kg, iv) e perfundidos transcardially com 0,1 M PBS até que esteja completamente exsanguinated.This ?...

<ol>
	<li>Gallagher, M., Rapp, P. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+animal+models+to+study+the+effects+of+aging+on+cognition.+Annu+Rev+Psychol+48.">The use of animal models to study the effects of aging on cognition. Annu Rev Psychol 48.</a> , 339-370 (1997).</li><li>Clancy, B., Darlington, R., Finlay, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Translating+developmental+time+across+mammalian+species.">Translating developmental time across mammalian species.</a> Neuroscience. 105, 7-17 (2001).</li><li>Nowakowski, R. 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N., Guzman-Flores, C., Juarez, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Voluntary+consumption+of+beverage+alcohol+by+vervet+monkeys:+population+screening,+descriptive+behavior+and+biochemical+measures.">Voluntary consumption of beverage alcohol by vervet monkeys: population screening, descriptive behavior and biochemical measures.</a> Pharmacol Biochem Behav. 36, 367-373 (1990).</li><li>Palmour, R. M., Mulligan, J., Howbert, J. 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O St. Kitts vervet (Chlorocebus aethiops sabeus) é um primata do Velho Mundo com padrões semelhantes e taxas de desenvolvimento do cérebro corticais e subcorticais ao dos humanos. Esta espécie tem sido utilizada para modelar humano complexo distúrbios comportamentais como comportamento ansioso, hipertensão 8, 9 hemisferectomia, doença de Parkinson 10, doença Alzhemier de 11, e abuso de álcool 12. Mais recentemente, esta espécie tem sido usado para estudar os...

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dissecting the non-human primate brain in stereotaxic space

O uso de primatas não-humanos fornece um excelente modelo de translação para a nossa compreensão dos processos de desenvolvimento e envelhecimento em seres humanos<sup&gt; 06/01</sup&gt;. Além disso, o uso de primatas não-humanos recentemente a oportunidade de, naturalmente, modelo complexo transtornos psiquiátricos, como o abuso de álcool<sup&gt; 7</sup&gt;. Aqui nós descrevemos uma técnica para bloquear o cérebro no plano coronal do macaco vervet (<em&gt; Chlorocebus aethiops sabeus</em&gt;) No crânio intacto no espaço estereotáxico. O método descrito aqui fornece um plano padrão de seção entre os blocos e assuntos, minimiza seções parciais entre os blocos. Seccionamento de um bloco de tecido no plano coronal também facilita a delimitação de uma área de interesse. Este método fornece gerenciáveis ​​blocos de tamanho desde um único hemisfério do macaco vervet produz mais de 1.200 seções quando cortar em 50 ìm. Além disso, bloqueando o cérebro em blocos de um centímetro, facilita a penetração da sacarose cyroprotection e permite que o bloco a ser cortado em um criostato padrão.

Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

Dissecting the Non-human Primate Brain in Stereotaxic Space

O primata não-humano é uma importante espécie de translação para a nossa compreensão do processamento normal do cérebro. A organização anatômica do cérebro dos primatas podem fornecer importantes insights sobre condições normais e patológicas nos seres humanos.

Dissecando o cérebro do primata não-humano no espaço estereotáxico

The use of non-human primates provides an excellent translational model for our understanding of developmental and aging processes in humans1-6. In ...

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Neurotransmission is the process by which neurons transfer information via chemical signals to their post-synaptic targets, on a rapid time scale.   This ...

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The non-human primate is an important translational species for understanding the normal function and disease processes of the human brain. Unbiased ...

Trajectory Data Analyses for Pedestrian Space-time Activity Study

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It is well recognized that human movement in the  spatial and temporal dimensions has direct influence on disease transmission1-3.  An infectious disease ...

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Blood samples are commonly obtained in many experimental contexts to measure targets of interest, including hormones, immune factors, growth factors, ...

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Renal micropuncture and renal 2-photon imaging are seminal techniques in renal physiology. However, micropuncture is limited by dependence on conventional ...

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Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

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Ultrafast force-clamp spectroscopy (UFFCS) is a single molecule technique based on laser tweezers that allows the investigation of the chemomechanics of ...