Name: dissecting the non-human primate brain in stereotaxic space
Uploaded: 2009-07-16T20:00:00
Description: Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

Författarna vill tacka Ikiel Ptito för hans fortsatta teknisk support. NSERC bidrag till MP.

Del 1: Förbehandling av vävnad <ol><li> Vävnad skall vara väl perfusion med paraformaldehyd, glutaraldehyd eller formalin. Detta kan uppnås genom vanliga transcardial perfusion vanligtvis används för att skörda andra organ. I den aktuella studien i ämnet var djupt sederad med ketamin hydroklorid (10 mg / kg im), avlivas med en överdos av natrium pentobarbital (25 mg / kg, iv) och perfusion transcardially med 0,1 M PBS tills den är helt exsanguinated.This följs med en 4% paraformaldehyd lösning i PBS i 5 m...

<ol>
	<li>Gallagher, M., Rapp, P. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+animal+models+to+study+the+effects+of+aging+on+cognition.+Annu+Rev+Psychol+48.">The use of animal models to study the effects of aging on cognition. Annu Rev Psychol 48.</a> , 339-370 (1997).</li><li>Clancy, B., Darlington, R., Finlay, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Translating+developmental+time+across+mammalian+species.">Translating developmental time across mammalian species.</a> Neuroscience. 105, 7-17 (2001).</li><li>Nowakowski, R. 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N., Guzman-Flores, C., Juarez, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Voluntary+consumption+of+beverage+alcohol+by+vervet+monkeys:+population+screening,+descriptive+behavior+and+biochemical+measures.">Voluntary consumption of beverage alcohol by vervet monkeys: population screening, descriptive behavior and biochemical measures.</a> Pharmacol Biochem Behav. 36, 367-373 (1990).</li><li>Palmour, R. M., Mulligan, J., Howbert, J. 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St Kitts vervet (Chlorocebus aethiops sabeus) är en gammal värld primat med liknande mönster och skattesatser i kortikala och subkortikala hjärnans utveckling den för människor. Denna art har använts för att modellera komplexa mänskliga beteendestörningar såsom ångest beteende, hypertoni 8, hemispherectomy 9, Parkinsons sjukdom 10, Alzhemier sjukdom 11 och missbruk av alkohol 12. På senare tid har denna art använts för att studera neuroanatom...

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dissecting the non-human primate brain in stereotaxic space

Användningen av icke-mänskliga primater är en utmärkt translationella modell för vår förståelse av utvecklings-och åldringsprocesser hos människor<sup&gt; 1-6</sup&gt;. Dessutom har användningen av icke-mänskliga primater ges nyligen tillfälle att naturligt modellera komplexa psykiatriska tillstånd såsom alkoholmissbruk<sup&gt; 7</sup&gt;. Här beskriver vi en teknik för att blockera hjärnan i coronal plan grön markatta (<em&gt; Chlorocebus aethiops sabeus</em&gt;) I skallbenet i stereotaxic rymden. Den metod som beskrivs här ger en vanlig plan sträckan mellan block och ämnen och minimerar partiell sektioner mellan blocken. Sektionering ett block av vävnad i koronala plan underlättar också avgränsningen av ett område av intresse. Denna metod ger hanterbar storlek block eftersom en enda halvklot av grön markatta ger mer än 1200 delarna när skivning på 50μm. Vidare genom att blockera hjärnan i 1 cm block, det underlättar penetrationen av sackaros för cyroprotection och låter det block som ska skivas på en standard kryostat.

Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

Dissecting the Non-human Primate Brain in Stereotaxic Space

Den icke-mänskliga primater är en viktig translationella art för vår förståelse av normal bearbetning av hjärnan. Den anatomiska organisation primater hjärnan kan ge viktiga insikter i normala och patologiska tillstånd hos människor.

Gå igenom Icke-mänskliga primater Brain i Stereotaxic rymden

The use of non-human primates provides an excellent translational model for our understanding of developmental and aging processes in humans1-6. In ...

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