Name: dissecting the non-human primate brain in stereotaxic space
Uploaded: 2009-07-16T20:00:00
Description: Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

Yazarlar Ikiel Ptito onun devam eden teknik destek için teşekkür etmek istiyorum. MP NSERC hibe.

Bölüm 1: Ön-işleme doku <ol><li> Doku paraformaldehid, glutaraldehid, ya da formalin ile perfüze edilmelidir. Bu, genellikle diğer organlarda hasat için kullanılan standart transcardial perfüzyon yoluyla elde edilebilir. Bu çalışmada konu derinden sodyum pentobarbital (25 mg / kg, iv), aşırı dozda ötenazi ketamin hidroklorür (10 mg / kg, im), sedasyon ve tamamen exsanguinated.This takip olana kadar 0.1 M PBS ile transcardially perfüze 5 dakika (~ 1 litre) PBS içinde% 4 paraformaldehid çözüm. </li...

<ol>
	<li>Gallagher, M., Rapp, P. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+animal+models+to+study+the+effects+of+aging+on+cognition.+Annu+Rev+Psychol+48.">The use of animal models to study the effects of aging on cognition. Annu Rev Psychol 48.</a> , 339-370 (1997).</li><li>Clancy, B., Darlington, R., Finlay, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Translating+developmental+time+across+mammalian+species.">Translating developmental time across mammalian species.</a> Neuroscience. 105, 7-17 (2001).</li><li>Nowakowski, R. 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P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tempo+of+neurogenesis+and+synaptogenesis+in+the+primate+cingulate+mesocortex:+comparison+with+the+neocortex.">Tempo of neurogenesis and synaptogenesis in the primate cingulate mesocortex: comparison with the neocortex.</a> J Comp Neurol. 360, 363-376 (1995).</li><li>Zecevic, N., Rakic, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+layer+I+neurons+in+the+primate+cerebral+cortex.">Development of layer I neurons in the primate cerebral cortex.</a> J Neurosci. 21, 5607-5619 (2001).</li><li>Ervin, F. R., Palmour, R. M., Young, S. N., Guzman-Flores, C., Juarez, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Voluntary+consumption+of+beverage+alcohol+by+vervet+monkeys:+population+screening,+descriptive+behavior+and+biochemical+measures.">Voluntary consumption of beverage alcohol by vervet monkeys: population screening, descriptive behavior and biochemical measures.</a> Pharmacol Biochem Behav. 36, 367-373 (1990).</li><li>Palmour, R. M., Mulligan, J., Howbert, J. 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St Kitts vervet (Chlorocebus aethiops sabeus) insanların kortikal ve subkortikal beyin gelişiminde benzer alışkanlıkları ve oranları ile bir Eski Dünya primat. Bu tür gibi karmaşık insan davranış bozuklukları endişeli davranış, hipertansiyon 8, hemisferektomi 9, Parkinson hastalığı 10, Alzhemier hastalığında 11 ve alkol kötüye kullanımı 12 modeli için kullanılır olmuştur. Daha yakın zamanlarda, bu tür natüralist prenatal et...

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<td>Scalpel</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>10003-12</td>
<td>&nbsp;</td>
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<td>Scalpel blades</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>10011-00</td>
<td>&nbsp;</td>
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<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>14090-11</td>
<td>Any surgical scissors are sufficient</td>
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<td>Rongeurs</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
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dissecting the non-human primate brain in stereotaxic space

Insan olmayan primatlardan, insanlarda gelişim ve yaşlanma süreçleri anlamak için mükemmel bir translasyonel modeli sağlar<sup&gt; 1-6</sup&gt;. Buna ek olarak, insan olmayan primatlardan kullanımı son zamanlarda, alkol bağımlılığı gibi karmaşık psikiyatrik bozukluklar doğal olarak modellemek için fırsat sağladığı vardır<sup&gt; 7</sup&gt;. Burada koronal düzlemde vervet maymun beyni bloke etmek için bir tekniktir (<em&gt; Chlorocebus aethiops sabeus</em&gt;) Stereotaksik alanı bozulmamış bir kafatası. Bu yöntem, burada blok ve bloklar arasındaki konular ve en aza indirir kısmi bölümleri arasındaki bölümde standart bir düzlemde sağlar nitelendirdi. Koronal düzlemde bir doku blok Kesit de bir ilgi alanı tarif kolaylaştırır. Bu yöntem, 50μm az dilimleme vervet maymun tek bir yarımkürede 1200&#39;den fazla bölüm verir yönetilebilir büyüklükte bloklar sağlar. Ayrıca beyin 1cm bloklar halinde bloke ederek, cyroprotection için sakaroz penetrasyonu kolaylaştırır ve blok standart bir Kriyostat dilimlenmiş olanak sağlar.

Watch this Scientific Journal Video about Dissecting the Non-human Primate Brain in Stereotaxic Space at JoVE.com

Dissecting the Non-human Primate Brain in Stereotaxic Space

Insan dışı primat beynin normal işleme anlamak için önemli bir translasyonel türüdür. Primat beynin anatomik organizasyonu, insanlarda normal ve patolojik durumları önemli bilgiler sağlayabilir.

Sigara insan Stereotaksik Uzay Primate Beyin Diseksiyon

The use of non-human primates provides an excellent translational model for our understanding of developmental and aging processes in humans1-6. In ...

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Neurotransmission is the process by which neurons transfer information via chemical signals to their post-synaptic targets, on a rapid time scale.   This ...

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Knowing What Counts: Unbiased Stereology in the Non-human Primate Brain

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The non-human primate is an important translational species for understanding the normal function and disease processes of the human brain. Unbiased ...

Trajectory Data Analyses for Pedestrian Space-time Activity Study

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It is well recognized that human movement in the  spatial and temporal dimensions has direct influence on disease transmission1-3.  An infectious disease ...

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Renal micropuncture and renal 2-photon imaging are seminal techniques in renal physiology. However, micropuncture is limited by dependence on conventional ...

Reverse Dissection and DiceCT Reveal Otherwise Hidden Data in the Evolution of the Primate Face

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Facial expressions, or facial displays, of social or emotional intent are produced by many mammalian taxa as a means of visually communicating with ...

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Situ Hibridizasyonda İki Renkli Tek Moleküllü Floresan Kullanarak Escherichia coli'de Uzay ve Zamanda MRNA KinetikLerini Sondalama

Single-molecule fluorescence in situ hybridization (smFISH) allows for counting the absolute number of mRNAs in individual cells. Here, we describe an ...

Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy

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Ultrafast force-clamp spectroscopy (UFFCS) is a single molecule technique based on laser tweezers that allows the investigation of the chemomechanics of ...