Name: brain banking: making the most of your research specimens
Uploaded: 2009-07-24T16:02:00
Description: Watch this Scientific Journal Video about Brain Banking: Making the Most of your Research Specimens at JoVE.com

<p class="jove_content">作者想感谢他持续的技术支持Ikiel Ptito。 </p>

<p class="jove_title">第1部分：前处理的组织</p><ol><li>组织应多聚甲醛，戊二醛，或福尔马林灌注。这可以通过标准transcardial，通常用于收获其他器官灌注。在本研究的主题是深刻的盐酸氯胺酮（10毫克/公斤，IM）与戊巴比妥钠（25毫克/公斤，四）过量安乐死，镇静剂和用0.1 M PBS灌注，直到完全exsanguinated transcardially人。其次是在PBS的4％多聚甲醛为5分钟（约1升）的解决方案。 </li><li>大脑应stereotaxically封锁，从头骨取出?...

<ol>
	<li>West, M., Slomianka, L., Gundersen, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Unbiased+stereological+estimation+of+the+total+number+of+neurons+in+the+subdivisions+of+the+rat+hippocampus+using+the+optical+fractionator.+Aat+Rec.">Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator. Aat Rec.</a> <b>231</b>, 482-497 (1991).</li><li>Burke, M. W., Zangenehpour, S., Boire, D., Ptito, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dissecting+the+Non-human+Primate+Brain+in+Stereotaxic+Space.">Dissecting the Non-human Primate Brain in Stereotaxic Space.</a> <em style='font-style: italic'>J Vis Exp</em>. , (2009).</li></ol>

<p class="jove_content">系统抽样是一种廉价的方法，旨在最大限度地提高研究材料。此取样策略的目的是，遵守的规则，需要整个地区的系统采样的无偏体视。至关重要的是，每个系列的部分路段秩序维持。在我们的实验室中，我们已经成功地使用银行仓鼠和非人类灵长类动物的脑切片这种方法。到目前为止，我们已经收集了接近20000 vervet猴<em>（Chlorocebus aethiops</em> sabeus）的脑切片，进行常规免疫组已存放了一年多的?...

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brain banking: making the most of your research specimens

<p class="jove_content">无偏体视学是一种准确，有效地估算总神经元的数量（或其他类型的细胞）在特定地区的利益的方法<sup&gt; 1</sup&gt;。要实现这一目标6-10探测系统部分应涵盖整个结构。通常情况下，这涉及到处理的1 / 5的部分叶片大量未处理的材料。为了最大限度地利用材料，我们提出了一个便宜的方法保留作为一个长期的存储研究计划的一部分的固定的组织。作为组织切片和处理所需的染色或抗体，替代部分应系统地放置在抗原在-20 ° C保存以备将来处理。利用24孔板，部分可以放置，以便将来检索。使用这种方法，组织可以存储和近两年的过程中的免疫组织化学处理。</p>

Watch this Scientific Journal Video about Brain Banking: Making the Most of your Research Specimens at JoVE.com

Brain Banking: Making the Most of your Research Specimens

<p class="jove_content">脑银行和生物材料的系统抽样提供无偏体视学的基础上，并最大限度地从每个试样获得潜在的数据。</p>

脑银行业：使你的研究样本中，大部分

Unbiased stereology is a method for accurately and efficiently estimating the total neuron number (or other cell type) in a given area of interest1.  To ...

Research

JoVE Journal

Biology

Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region

从特定的大脑区域的RNA或蛋白质提取大鼠脑微解剖

Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates ...

科研

生物学

Single Cell Electroporation in vivo within the Intact Developing Brain

在体内的单细胞电穿孔内完整的大脑发育

Single-cell electroporation (SCE) is a specialized technique allowing the delivery of DNA or other macromolecules into individual cells within intact ...

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

膜片吸液管和夏普电极与一个可编程的拖轮

Glass microelectrodes (also called pipettes) have been a workhorse of electrophysiology for decades.  Today, such pipettes are made from glass capillaries ...

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

现场共聚焦显微镜对斑马鱼的胚胎脑成像

In this video, we demonstrate the method our lab has developed to analyze the cell shape changes and rearrangements required to bend and fold the ...

Making MR Imaging Child's Play - Pediatric Neuroimaging Protocol, Guidelines and Procedure

使磁共振成像儿戏 - 小儿神经影像学的协议，指引及程序

Within the last decade there has been an increase in the use of structural and functional magnetic resonance imaging (fMRI) to investigate the neural ...

Using the Horseshoe Crab, <em>Limulus Polyphemus</em>, in Vision Research

Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research

马蹄蟹，<em>鲎波吕斐摩斯</em>，在视觉研究

The American horseshoe crab, Limulus Polyphemus is one of the oldest creatures on earth, and the animal continues to play an indispensable role in ...

Making Gynogenetic Diploid Zebrafish by Early Pressure

初压雌核发育二倍体的斑马鱼

Heterozygosity in diploid eukaryotes often makes genetic studies cumbersome. Methods that produce viable homozygous diploid offspring directly from ...

Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography

长期培养的人类乳腺癌标本，并利用光学投影层析成像分析

Breast cancer is a leading cause of mortality in the Western world. It is well established that the spread of breast cancer, first locally and later ...

Obtaining Specimens with Slowed, Accelerated and Reversed Aging in the Honey Bee Model

获取标本减慢，加速和反老化的蜜蜂型号

Societies of highly social animals feature vast lifespan differences between closely related individuals. Among social insects, the honey bee is the best ...

Sampling Blood from the Lateral Tail Vein of the Rat

从大鼠的侧尾静脉血液取样

Blood samples are commonly obtained in many experimental contexts to measure targets of interest, including hormones, immune factors, growth factors, ...