Name: the gateway to the brain: dissecting the primate eye
Uploaded: 2009-05-27T00:00:00
Description: Watch this Scientific Journal Video about The Gateway to the Brain: Dissecting the Primate Eye at JoVE.com

<p class="jove_content">作者想感谢他的技术支持Ikiel Ptito。弗兰克欧文，罗伯塔Palmour和行为科学基金会设在圣基茨实验室，西印度群岛，为他们继续支持我们的灵长类动物的工作人员，我们非常感谢。 </p>

<p class="jove_title">第1部分：前处理的组织</p><ol><li>组织应多聚甲醛，戊二醛，或福尔马林灌注。这可以通过标准transcardial，通常用于收获其他器官灌注。建议后不久牺牲的眼睛被注入固定液，只是镜头下的固定液中。 </li><li>在本研究的主题是深刻的盐酸氯胺酮（10毫克/公斤，IM）与戊巴比妥钠（25毫克/公斤，四）过量安乐死，镇静剂和用0.1 M PBS灌注，直到完全exsanguinated transcardially人。其次是在PBS的4％多聚甲醛为5?...

<ol>
	<li>Hendrickson, A., Kupfer, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+histogenesis+of+the+fovea+in+the+macaque+monkey.">The histogenesis of the fovea in the macaque monkey.</a> <em style='font-style: italic'>Invest Ophthalmol Vis Sci</em>. <b>15</b>, 746-756 (1976).</li><li>Herbin, M., Boire, D., Ptito, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Size+and+distribution+of+retinal+ganglion+cells+in+the+St.+Kitts+green+monkey+(Cercopithecus+aethiops+sabeus).">Size and distribution of retinal ganglion cells in the St. Kitts green monkey (Cercopithecus aethiops sabeus).</a> <em style='font-style: italic'>J Comp Neurol</em>. <b>383</b>, 459-472 (1997).</li><li>Stone, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>The Wholemount Handbook</em>. , (1981).</li><li>Herbin, M., Boire, D., Theoret, H., Ptito, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transneuronal+degeneration+of+retinal+ganglion+cells+in+early+hemispherectomized+monkeys.">Transneuronal degeneration of retinal ganglion cells in early hemispherectomized monkeys.</a> <em style='font-style: italic'>Neuroreport</em>. <b>10</b>, 1447-1452 (1999).</li><li>Krebs, W., Krebs, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Primate Retina and Choroid Atlas of Fine Structure in Man and Monkey</em>. , (1991).</li><li>Pow, D., Sullivan, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nuclear+kinesis,+neurite+sprouting+and+abnormal+axonal+projections+of+cone+photoreceptors+in+the+aged+and+AMD-afflicted+human+retina.">Nuclear kinesis, neurite sprouting and abnormal axonal projections of cone photoreceptors in the aged and AMD-afflicted human retina.</a> <em style='font-style: italic'>Exp Eye Res</em>. <b>84</b>, 850-857 (2007).</li></ol>

<p class="jove_content">准备允许作为wholemount视网膜的神经节细胞层，或视网膜<sup>血管</sup> 3内皮细胞的地形和空间分布的分析。在灵长类动物的视网膜边缘的细胞密度的量化很容易完成。然而，在perifoveal和中心凹区域，堆积在神经节细胞层的多层次的阻碍量化。为了规避这种潜在的偏见，黄斑中心凹和perifoveal地区可以解剖从wholemount准备，嵌在EPON，并连续切片使用ultramicrotome获得半薄切片，在冠状<sup>面</sup> 2,4 。有w...

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the gateway to the brain: dissecting the primate eye

<p class="jove_content">在人类的视觉系统被认为是世界的门户和过多的感官，知觉和认知过程中起着主要作用。因此，并不令人惊讶的视觉质量与生活质量。尽管广泛的临床和基础研究周围的视觉障碍的原因，许多形式如色素性视网膜炎和黄斑变性，视力障碍，缺乏有效的治疗方法。非人类灵长类动物的最接近人类的眼球发育一般特征。他们不仅有类似的血管解剖，但其他哺乳动物，包括灵长类动物有独特的特征，在一个区域专门用于高视力，黄斑中心凹视网膜颞<sup&gt; 1</sup&gt;。在这里，我们描述了一个解剖的灵长类动物的视网膜提供视网膜组织学，免疫组织化学，激光捕获显微切割技术，以及光学和电子显微镜的组织，一般技术。随着使用的非人类灵长类动物作为一个翻译模型的扩展，我们希望的是更好地理解视网膜将提供有效的方法以减轻或扭转受影响的个人的生活质量产生负面影响视觉障碍的见解。</p>

Watch this Scientific Journal Video about The Gateway to the Brain: Dissecting the Primate Eye at JoVE.com

The Gateway to the Brain: Dissecting the Primate Eye

<p class="jove_content">非人类灵长类动物是我们的发育和衰老的理解的重要翻译物种。灵长类动物视网膜的解剖组织可能为在人体正常和病理条件下的重要见解。</p>

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