Name: measuring plant cell wall extension (creep) induced by acidic ph and by alpha-expansin
Uploaded: 2009-03-11T00:00:00
Description: Watch this Scientific Journal Video about Measuring Plant Cell Wall Extension Creep Induced by Acidic pH and by Alpha-Expansin at JoVE.com

这里显示的技术开发资金从能源部生物能源科学和国家科学基金会的赠款。

第1部分：成长和存储合适的植物材料<ol><li>根据我们的经验，年轻的黄化黄瓜幼苗下胚轴作为这些实验的细胞壁物质来源方便。黄瓜种子播种在湿纸在避光盒子，里面是在黑暗的内阁保持在设定房间温度恒定在26 ° C。精确的温度并不重要，为22 °和30 °之间的任何C应被罚款，但温度将决定如何快速苗达到一个适当的发展阶段。温暖的温度，更快的幼苗发展。我们通常使用时，他们已发展到约5厘?...

<ol>
	<li>Cosgrove, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+long-term+extension+of+isolated+cell+walls+from+growing+cucumber+hypocotyls.">Characterization of long-term extension of isolated cell walls from growing cucumber hypocotyls.</a> Planta. 177, 121-130 (1989).</li><li>Rayle, D. L., Cleland, R. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Acid+Growth+Theory+of+auxin-induced+cell+elongation+is+alive+and+well.">The Acid Growth Theory of auxin-induced cell elongation is alive and well.</a> Plant Physiol. 99, 1271-1274 (1992).</li><li>Cleland, R. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Auxin-induced+growth+of+Avena+coleoptiles+involves+two+mechanisms+with+different+pH+optima.">Auxin-induced growth of Avena coleoptiles involves two mechanisms with different pH optima.</a> Plant Physiol. 99, 1556-1561 (1992).</li><li>Cosgrove, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Loosening+of+plant+cell+walls+by+expansins.">Loosening of plant cell walls by expansins.</a> Nature. 407, 321-326 (2000).</li><li>McQueen-Mason, S., Durachko, D. M., Cosgrove, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two+endogenous+proteins+that+induce+cell+wall+expansion+in+plants.">Two endogenous proteins that induce cell wall expansion in plants.</a> Plant Cell. 4, 1425-1433 (1992).</li></ol>

对于本演示中，我们用黄瓜下胚轴细胞壁，因为他们已经被证明是一个可靠的来源，很容易处理，并具有良好的灵敏度，响应墙样品。我们也有过良好的成功与其他树苗以及一些材料从超市，如年轻的菠菜叶和芹菜，墙壁。基本上，年轻，柔软，生长迅速的植物组织有可能使用这种技术可以很容易地测量，但坚韧，老，氧化或nongrowing的植物组织都不太可能因为细胞壁交联反应。有一些其他的东西...

measuring plant cell wall extension (creep) induced by acidic ph and by alpha-expansin

日益严重的植物细胞壁的特征表现为“酸的增长”，我们的意思，他们是在低pH值（&lt;5）1更具可扩展性着称的属性。植物激素生长素迅速刺激至少有一部分细胞伸长酸增长机制，2，3，幼茎和类似组织。生长素激活的H +泵的质膜，造成细胞壁溶液酸化。长城酸化激活expansins，这是内源性的细胞壁松蛋白 4，导致细胞壁产生细胞膨压在墙壁上创建的紧张局势。因此，细胞开始迅速放大。这“酸增长”的现象很容易测量隔离（非生物）细胞壁标本。细胞壁的能力进行酸诱导延伸不是单纯的细胞壁多糖（如果胶）的结构安排的结果，而是取决于活动 expansins 5。 Expansins没有任何已知的酶的活性，并检测膨大活动的唯一途径，是衡量其细胞壁扩展的感应。这个视频报告的细节膨大实验获得合适的墙体材料的来源和制备技术及酸诱导的延伸和膨大诱导墙样品准备种植黄瓜下胚轴的延伸。 要获得适当的细胞壁样品，黄瓜幼苗在黑暗中生长，胚轴削减和冻结在-80 ° C。冷冻胚轴磨损，夷为平地，然后在引伸计测量的一个特殊的比色皿的恒张力夹紧。为了测量酸诱导的延伸，墙壁最初在中性pH缓冲，导致在低活动expansins本地细胞壁的组成部分。当酸性pH值的缓冲液交换，ex​​pansins被激活和细胞壁迅速扩大。我们也证明了一个重组实验膨大活动。对于这部分，我们使用一个简短的热处理，在细胞壁样变性的原生expansins。这些灭活的细胞壁不延长，甚至在酸性的缓冲，但除了细胞壁expansins迅速恢复他们的能力，延长。

Watch this Scientific Journal Video about Measuring Plant Cell Wall Extension Creep Induced by Acidic pH and by Alpha-Expansin at JoVE.com

Measuring Plant Cell Wall Extension Creep Induced by Acidic pH and by Alpha-Expansin

我们证明一个恒定的力量引伸来衡量长期，延长植物细胞壁标本（蠕变）酸性缓冲区和膨大蛋白质引起的。

酸性α-膨大诱导测量植物细胞壁扩展（蠕变）

Growing plant cell walls characteristically exhibit a property known as 'acid growth', by which we mean they are more extensible at low pH (&lt; 5) 1. The ...

Research

JoVE Journal

Biology

Measuring Plant Cell Wall Extension (Creep) Induced by Acidic pH and by Alpha-Expansin

Title Cell Encapsulation by Droplets

通过飞沫标题细胞封装

科研

生物学

Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato

Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato

病毒诱导的基因沉默（VIGS）烟草benthamiana和番茄

RNA interference (RNAi) is a highly specific gene-silencing phenomenon triggered by dsRNA1. This silencing mechanism uses two major classes of RNA ...

Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast

细菌III型效应，在酵母中的表达诱导的生长抑制的表型鉴定

Many Gram-negative pathogenic bacteria use a type III secretion system to translocate a suite of effector proteins into the cytosol of host cells. Within ...

Agar-Block Microcosms for Controlled Plant Tissue Decomposition by Aerobic Fungi

为控制植物组织分解好氧菌的琼脂块缩影

The two principal methods for studying fungal biodegradation of lignocellulosic plant tissues were developed for wood preservative testing (soil-block; ...

Pouring and Running a Protein Gel by reusing Commercial Cassettes

浇注和运行的一种蛋白质凝胶重用商​​业磁带

The evaluation of proteins using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis is a common technique used by biochemistry ...

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

糖基谱研究植物细胞壁聚合物，用微阵列

Plant cell walls are complex matrixes of heterogeneous glycans which play an important role in the physiology and development of plants and provide the ...

Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture

隔离原住民土壤微生物潜力打破生物降解塑料地膜应用于农业

Fungi native to agricultural soils that colonized commercially available biodegradable mulch (BDM) films were isolated and assessed for potential to ...

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪

Through a delicate balance between quiescence and proliferation, self renewal and production of differentiated progeny, hematopoietic stem cells (HSCs) ...

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

糖皮质激素粪分析：在马属动物无创监测肾上腺

Adrenal activity can be assessed in the equine species by analysis of feces for corticosterone metabolites. During a potentially aversive situation, ...

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications

结晶纤维素积累高分辨率的量化<em&gt;拟南芥</em&gt;根监视特定组织细胞壁修改

Plant cells are surrounded by a cell wall, the composition of which determines their final size and shape. The cell wall is composed of a complex matrix ...