Name: mouse mammary epithelial cells form mammospheres during lactogenic differentiation
Uploaded: 2009-10-06T16:30:00
Description: Watch this Scientific Journal Video about Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation at JoVE.com

<p class="jove_content">从国会导演医学研究基金资助（DAMD17 - 01 - 0264）ML卡特勒和美国军事癌症研究所ML卡特勒的资金支持工作。 </p>

<ol><li> 6天的RPMI 1640培养基中，10％小牛血清，5μg/ml胰岛素，10毫米的HEPES，在10ng/ml表皮生长因子（EGF），建立能力，HC11细胞生长汇合。 </li><li>细胞用PBS洗涤，并保持24小时，在没有表皮生长因子生长介质。 </li><li>为了诱导lactogenic分化的细胞培养分化的媒体，即地塞米松（10 <sup>-6 M）</sup>的RPMI，胰岛素（5微克/毫升）和催乳素（5微克/毫升），简称为DIP 。 </li><li>在规定的时间后DIP此外，微球体进行了拍照，并列举...

<ol>
	<li>Blatchford, D. R., Hendry, K. A., Turner, M. D., Burgoyne, R. D., Wilde, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vectorial+secretion+by+constitutive+and+regulated+secretory+pathways+in+mammary+epithelial+cells.">Vectorial secretion by constitutive and regulated secretory pathways in mammary epithelial cells.</a> <em style='font-style: italic'>Epithelial Cell Biol</em>. <b>4</b>, 8-16 (1995).</li><li>Wang, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Glucocorticoid+induced+expression+of+connective+tissue+growth+factor+contributes+to+lactogenic+differentiation+of+mouse+mammary+epithelial+cells.">Glucocorticoid induced expression of connective tissue growth factor contributes to lactogenic differentiation of mouse mammary epithelial cells.</a> <em style='font-style: italic'>J Cell Physiol</em>. <b>214</b>, 38-46 (2008).</li></ol>

<p class="jove_content">为了使这项技术获得成功，最好的质量，必须保持上皮细胞在合流之前被lactogenic激素诱导。有可能有无数的微球体，这使得难以量化的情况下。因此，定量的目的，最好在一个时间点拍摄时的微球体，能算的微球体。 </p><p class="jove_content">虽然有各种分子标记，用于确定乳腺上皮细胞的lactogenic分化程度，有几个用于2D文化细胞生长的形态标记。上皮细胞单层的能力，形成微型穹顶结构，牛奶蛋白?...

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mouse mammary epithelial cells form mammospheres during lactogenic differentiation

<p class="jove_content">常用的表型措施，以确定在小鼠乳腺上皮细胞培养的lactogenic分化程度是圆顶形细胞结构的形成，被称为微球体<sup&gt; 1</sup&gt;。 HC11细胞系作为模型系统已受聘乳腺lactogenic分化调控的研究<em&gt;在体外</em&gt;<em&gt;在体内</em<sup&gt; 2</sup&gt;。 HC11细胞分化和综合lactogenic激素治疗牛奶蛋白。 HC11小鼠乳腺上皮合流细胞的生长，lactogenic分化诱导此外lactogenic激素，包括地塞米松，胰岛素和催乳素的组合，简称为DIP。 HC11细胞诱导分化的拍摄时间可达120个小时的分化诱导后的微球体出现在每一种文化，是列举。个人微球体的大小，分化程度相关，这是区分细胞的图像描绘。</p>

Watch this Scientific Journal Video about Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation at JoVE.com

Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation

<p class="jove_content">HC11 lactogenic分化的特点可以简称为微球体的半球形结构的形成。相衬显微镜的结构可以枚举援助量化lactogenic分化。</p>

小鼠乳腺上皮细胞形成过程中Lactogenic分化的微球体

A phenotypic measure commonly used to determine the degree of lactogenic differentiation in mouse mammary epithelial cell cultures is the formation of ...

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