Name: mouse mammary epithelial cells form mammospheres during lactogenic differentiation
Uploaded: 2009-10-06T16:30:00
Description: Watch this Scientific Journal Video about Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation at JoVE.com

<p class="jove_content">議会監督医学研究基金助成金（DAMD17 - 01から0264）からMLカトラーとMLカトラーへの米軍癌研究所の資金は、作業を支持した。 </p>

<ol><li> HC11細胞を10％ウシ胎児血清、5μg/mlインスリン、10mMのHEPES、能力を確立するために10ng/ml上皮成長因子（EGF）を添加したRPMI 1640培地で6日間コンフルエントまで増殖させた。 </li><li>細胞をPBSで洗浄し、24時間EGFのない増殖培地中で維持した。 </li><li>乳腺刺激分化細胞を分化培地中でインキュベートしたを誘導するために、デキサメタゾン（10 <sup>-6</sup> M）、インスリン（5μg/ mlの）とプロラクチン（5μg/ ml?...

<ol>
	<li>Blatchford, D. R., Hendry, K. A., Turner, M. D., Burgoyne, R. D., Wilde, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vectorial+secretion+by+constitutive+and+regulated+secretory+pathways+in+mammary+epithelial+cells.">Vectorial secretion by constitutive and regulated secretory pathways in mammary epithelial cells.</a> <em style='font-style: italic'>Epithelial Cell Biol</em>. <b>4</b>, 8-16 (1995).</li><li>Wang, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Glucocorticoid+induced+expression+of+connective+tissue+growth+factor+contributes+to+lactogenic+differentiation+of+mouse+mammary+epithelial+cells.">Glucocorticoid induced expression of connective tissue growth factor contributes to lactogenic differentiation of mouse mammary epithelial cells.</a> <em style='font-style: italic'>J Cell Physiol</em>. <b>214</b>, 38-46 (2008).</li></ol>

<p class="jove_content">この手法を成功させると最高の品質のためにためには、上皮細胞は乳腺刺激ホルモンで誘導される前に合流する地点に維持する必要があります。定量化が困難に多数のmammospheresが、ある状況があるかもしれません。従って、定量的な目的のためにそれはmammospheresの数がカウントされることができるとき時点でmammospheresを撮影するのが最善です。 </p><p class="jove_content">乳腺上皮細胞の乳腺刺激?...

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mouse mammary epithelial cells form mammospheres during lactogenic differentiation

<p class="jove_content">一般的にマウス乳腺上皮細胞培養における乳腺刺激分化の程度を決定するために使用される表現型の指標はmammospheresと呼ばれるドーム状のセル構造の形成である<sup&gt; 1</sup&gt;。 HC11細胞株は、乳腺乳腺刺激分化の両方の規制の研究のためのモデルシステムとして採用されている<em&gt; in vitroで</em&gt;と<em&gt; in vivoで</em<sup&gt; 2</sup&gt;。 HC11細胞は乳腺刺激ホルモンによる治療に反応して牛乳のタンパク質を区別して合成する。合流へHC11マウス乳腺上皮細胞の成長に続いて、乳腺刺激分化がデキサメタゾン、インスリン、およびDIPと呼ばれるプロラクチン、乳腺刺激ホルモンを含む組み合わせの添加により誘導した。分化誘導HC11細胞を120時間後に分化誘導し、列挙されたそれぞれの文化に登場mammospheresの数の倍、最大で撮影されました。個々のmammospheresの大きさは、分化の程度と相関し、これは、分化細胞の画像に描かれている。</p>

Watch this Scientific Journal Video about Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation at JoVE.com

Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation

<p class="jove_content">HC11乳腺刺激分化はmammospheresと呼ばれるドーム型の構造体の形成によって特徴づけすることができます。構造は、乳腺刺激分化を定量化を支援するために、位相差顕微鏡で列挙することができます。</p>

マウス乳腺上皮細胞は乳腺刺激分化の過程でMammospheresを形成し、

A phenotypic measure commonly used to determine the degree of lactogenic differentiation in mouse mammary epithelial cell cultures is the formation of ...

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