Name: dendra2 photoswitching through the mammary imaging window
Uploaded: 2009-06-05T00:00:00
Description: Watch this Scientific Journal Video about Dendra2 Photoswitching through the Mammary Imaging Window at JoVE.com

この作品は、米国国防総省（BGとBC061403にDKにBC075554）、米国国立衛生研究所（; JC、JESとJWへCA100324、JESにCA77522、U54CA126511 JCとBGにJVRにU54GM064346）によってサポートされていました。我々は、顕微鏡、イメージングボックスとF4/80抗体のためのJ.ポラード（医学のアルバートアインシュタイン大学）の製造で助けM. RottenkolberのヘルプはD. Entenbergに感謝。

1。乳腺脂肪体に腫瘍細胞の注入を用いて蛍光腫瘍の発生： <ol><li> 80％コンフルエント - 40にDendra2タンパク質を（細胞質マーカーなど）を発現する細胞株を成長する。 </li><li> PBS W / OのCa 2で料理を少なくとも3回リンス+やMg 2 +。 </li><li> ° C細胞のほとんどはそれを振るために平らな表面に皿をヒットしてデタッチとなるまで37℃で10cmディッシュとインキュベート当た?...

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	<li>Farina, K. L., Wyckoff, J. B., Rivera, J., Lee, H., Segall, J. E., Condeelis, J. S., Jones, J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+motility+of+tumor+cells+visualized+in+living+intact+primary+tumors+using+green+fluorescent+protein.">Cell motility of tumor cells visualized in living intact primary tumors using green fluorescent protein.</a> Cancer Res. 58, 2528-2532 (1998).</li><li>Naumov, G. N., Wilson, S. M., MacDonald, I., Schmidt, E. E., Morris, V. L., Groom, A. C., Hoffman, R. M., Chambers, A. 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dendra2 photoswitching through the mammary imaging window

シングルセルの解像度でマウスとラットの乳腺腫瘍の過去10年間で、生体顕微鏡<sup&gt; 1月4日</sup&gt;このような腫瘍細胞の遊走、浸潤や脈管内へのような転移挙動のメカニズムに重要な洞察をもたらした<sup&gt; 5、6</sup&gt;、血管新生<sup&gt; 3</sup&gt;と免疫細胞の応答<sup&gt; 7月9日</sup&gt;。我々は最近、生きているマウスでは複数の生体内イメージングセッションを介して画像同所性乳がんに手法を報告している<sup&gt; 10</sup&gt;。このため、我々はDendra2ための乳腺画像ウィンドウ（MIW）と最適化されたイメージングパラメータを開発している<sup&gt; 11</sup&gt; photoswitchingとイメージング<em&gt; in vivoで</em&gt;。ここで、我々はMIW、カスタム構築されたイメージングボックスを使用してDendra2標識腫瘍細胞の腫瘍と画像の上にMIWの挿入の製造のためのプロトコルについて説明します。このプロトコルは、画像の腫瘍の異なる微小環境における腫瘍細胞の転移挙動をするために使用され、血管、腫瘍細胞と宿主細胞の長期的なイメージングを可能にすることができます。

Watch this Scientific Journal Video about Dendra2 Photoswitching through the Mammary Imaging Window at JoVE.com

Dendra2 Photoswitching through the Mammary Imaging Window

乳腺画像ウィンドウを通してDendra2標識腫瘍細胞の生体内photoswitchingとトラッキングは、私たちは、画像間のタイムスケール上の選択された腫瘍の微小環境における腫瘍細胞の転移挙動をするという技術です。

乳腺イメージングの窓からDendra2 Photoswitching

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