Name: creation of murine experimental abdominal aortic aneurysms with elastase
Uploaded: 2009-07-23T18:00:00
Description: Watch this Scientific Journal Video about Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase at JoVE.com

<p class="jove_content">这项工作是支持SCCOR授予“AAA级疾病：机制，分层，与治疗”，由美国国立卫生研究院（P50 HL083800 - 03） </p>

<ol><li>小鼠麻醉与吸入异氟醚2.03.0毫升/ L。 </li><li>腹部是剃光，用酒精和优碘擦洗。 </li><li>鼠标放在仰卧于手术视野。 </li><li>无菌手套戴由外科医生，并已与热珠灭菌消毒的法器。 </li><li>是一个长期的中线腹部切口。外面用无菌纱布，露出腹主动脉腹部缩回腹腔内容。 </li><li>腹主动脉是孤立的左肾静脉水平分叉。 </li><li>外露，用10-0缝线结扎腹主动脉的分支。结扎主动脉分叉1.0厘米内的所有分行。 </li><li>临时6.0...

<ol>
	<li>Anidjar, S., Salzmann, J. L., Gentric, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Elastase-induced+experimental+aneurysms+in+rats.">Elastase-induced experimental aneurysms in rats.</a> <em style='font-style: italic'>Circulation</em>. <b>82</b> (3), 973-973 (1990).</li><li>Pyo, R., Lee, J. K., Shipley, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeted+gene+disruption+of+matrix+metalloproteinase-9+(gelatinase+B)+suppresses+development+of+experimental+abdominal+aortic+aneurysms.">Targeted gene disruption of matrix metalloproteinase-9 (gelatinase B) suppresses development of experimental abdominal aortic aneurysms.</a> <em style='font-style: italic'>J Clin Invest</em>. <b>105</b> (11), 1641-1641 (2000).</li><li>Carsten, C. G., Calton, W. C., Johanning, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Elastase+is+not+sufficient+to+induce+experimental+abdominal+aortic+aneurysms.">Elastase is not sufficient to induce experimental abdominal aortic aneurysms.</a> <em style='font-style: italic'>J Vasc Surg</em>. <b>33</b> (6), 1255-1255 (2001).</li></ol>

<p class="jove_content">以前的研究已经指出了显着的变化很多AAA级发展到很多不同的商业弹性<sup>蛋白酶</sup>制剂3。因此，至关重要的是，监察批量生产以及有相应的对照组，以文件的编制能力，促使美国科学促进会。 </p><p class="jove_content">有趣的是，在此模型中，AAA级发展的易感性是<sup>依赖</sup>于使用3的小鼠品系。在当前的视频用鼠标AAA一致的开发利用概述协议C57BL / 6株。弹性蛋白酶浓度和/或培养时间可能会有所?...

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<td>Type I porcine pancreatic elastase</td>
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<td>LEICA MZ6</td>
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<td>Leica</td>
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<td>LEICA DFC290HD</td>
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creation of murine experimental abdominal aortic aneurysms with elastase 

<p class="jove_content">瞬态猪胰弹性蛋白酶的腔内注入到下腹段腹主动脉腹主动脉瘤（AAA）是最广泛使用的动物模型，自从它第一次描述Anidjar和他的同事<sup>在</sup>大鼠1的理由是其发展的基于打乱弹性蛋白的性质，观察在美国科学促进会。此大鼠模型已被修改，在小鼠产生下腹主动脉地区的美国科学促进会<sup>。2</sup>模型的能力添加到病理学，由于出现了大量的转基因和基因敲除小鼠的AAA广泛的洞察力。此外，它是一个可行的的平台来测试潜在的治疗药物为AAA。在这个视频中，我们展示在我们的实验室中使用的弹性蛋白酶输液AAA过程。 </p><p class="jove_content">小鼠是使用2.5％异氟醚麻醉和开腹手术是在无菌条件下进行。腹部aortais独立经营体视显微镜（莱卡）的援助。在近端和远端的主动脉周围放置临时连字，一个aortotomy是建立在与30号针头的针尖分叉。一个PE - 10聚乙烯管材热锥形段是通过aortotomy介绍和担保。主动脉管腔随后灌注盐水含I型猪胰弹性蛋白酶（4.5 U /毫升; Sigma公司）在100毫米汞柱为5-15分钟。灌注导管取出后，aortotomy是无管腔收缩修复。 </p>

Watch this Scientific Journal Video about Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase at JoVE.com

Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase

<p class="jove_content">该视频演示了如何通过瞬态下腹段腹主动脉腔内灌注到猪胰弹性蛋白酶诱导小鼠腹主动脉瘤（AAA）的。该模型已添加到AAA的病理学由于出现了大量的转基因和基因敲除小鼠的广泛的洞察力的能力。</p>

创建小鼠实验与弹性蛋白酶的腹主动脉瘤

Transient intraluminal infusion of porcine pancreatic elastase into the infrarenal segment of the abdominal aorta is the most widely used animal model of ...

Research

JoVE Journal

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Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase 

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Trivial superficial wounds heal without complications by primary intention. Deep wounds, such as full thickness burns, heal by secondary intention and ...

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The liver is the largest organ in mammals. It plays an important role in glucose storage, protein secretion, metabolism and detoxification. As the ...