Name: mouse adrenal chromaffin cell isolation
Uploaded: 2007-01-05T00:00:00
Description: Watch this Scientific Journal Video about Mouse Adrenal Chromaffin Cell Isolation at JoVE.com

Afkortingen: ACC - bijnier Chromaffinecellen cellen, E. Soln - Enzyme Solution, E. DMEM - Verrijkt Dulbecco&#39;s Modified Eagle Medium <ol><li> Voorbereiding <ol><li> Voeg papaïne om de gewenste hoeveelheid van E. Soln (40 eenheden papaïne / 1ml E. Soln.). </li><li> Activeer papaïne met carbogen voor ongeveer 15 minuten op ijs. Zuurstof Lockes buffer op ijs ook. </li></ol></li><li> Ontleding</span...

mouse adrenal chromaffin cell isolation

Bijniermerg chromaffin celkweek systemen zijn uiterst nuttig voor de studie van de excitatie-secretie koppeling in een in vitro setting. Dit protocol geeft de methode die wordt gebruikt om de bijnieren ontleden en vervolgens de medullaire regio te isoleren door het strippen van weg de bijnierschors. De vertering van de medulla in enkele chroomaffiene cellen wordt dan aangetoond.

Watch this Scientific Journal Video about Mouse Adrenal Chromaffin Cell Isolation at JoVE.com

Mouse Adrenal Chromaffin Cell Isolation

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Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting.  This ...

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