Name: mouse adrenal chromaffin cell isolation
Uploaded: 2007-01-05T00:00:00
Description: Watch this Scientific Journal Video about Mouse Adrenal Chromaffin Cell Isolation at JoVE.com

Abkürzungen: ACC - Adrenal chromaffinen Zellen, E. Soln - Enzymlösung, E. DMEM - Enriched Dulbeccos Modified Eagle Medium <ol><li> Vorbereitung <ol><li> Add Papain auf die gewünschte Menge an E. Soln (40 Einheiten Papain / 1ml E. Soln.). </li><li> Aktivieren Papain mit Carbogen für etwa 15 Minuten auf dem Eis. Oxygenat Lockes Puffer auf Eis als auch. </li></ol></li><li> Zergliederung <ol><l...

mouse adrenal chromaffin cell isolation

Nebennierenmarks chromaffinen Zellkultur-Systeme sind äußerst nützlich für die Untersuchung der Anregungs-Sekretion Kopplung in einem in vitro-Einstellung. Dieses Protokoll stellt das Verfahren verwendet werden, um den Nebennieren zu sezieren und dann isolieren medulläre Region durch Abstreifen der Nebennierenrinde. Die Verdauung der Medulla in einzelne chromaffinen Zellen wird dann demonstriert.

Watch this Scientific Journal Video about Mouse Adrenal Chromaffin Cell Isolation at JoVE.com

Mouse Adrenal Chromaffin Cell Isolation

Maus Adrenal Chromaffin Zellisolation

Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting.  This ...

Research

JoVE Journal

Biology

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Cardiomyocytes, the workhorse cell of the heart, contain exquisitely organized cytoskeletal and contractile elements that generate the contractile force ...

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