Name: mouse adrenal chromaffin cell isolation
Uploaded: 2007-01-05T00:00:00
Description: Watch this Scientific Journal Video about Mouse Adrenal Chromaffin Cell Isolation at JoVE.com

Kısaltmalar: TKK - Adrenal Kromafin Hücreler, E. Soln - Enzim Çözüm, E. DMEM - Zenginleştirilmiş Dulbecco&#39;nun Modifiye Kartal Orta <ol><li> Hazırlık <ol><li> E. Soln (40 adet papain / 1 ml E. Soln.) Istenilen miktar papain ekleyin. </li><li> Carbogen ile yaklaşık 15 dakika boyunca buz üzerinde papain etkinleştirin. Oxygenate Locke buz tampon olarak. </li></ol></li><li> Diseksiyon</spa...

mouse adrenal chromaffin cell isolation

Adrenal medüller kromafin hücre kültürü sistemleri, in vitro ortamda bir uyarım-salgılanması kaplin çalışması için son derece yararlı. Bu protokol adrenaller incelemek ve sonra Adrenal korteks uzak sıyırma medüller bölgede izole etmek için kullanılan yöntemi göstermektedir. Tek kromafin hücrelerine medulla sindirim sonra gösterilmiştir.

Watch this Scientific Journal Video about Mouse Adrenal Chromaffin Cell Isolation at JoVE.com

Mouse Adrenal Chromaffin Cell Isolation

Fare Adrenal Kromafin Hücre İzolasyonu

Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting.  This ...

Research

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Biology

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