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DOI :
10.3791/1293-v
August 19th, 2009
Chapters
0:10
Introduction
0:42
Expression and Purification of Target Protein and Tagged Ubiquitin
3:15
Immunoprecipitation
4:51
SDS-PAGE and Immunostaining
5:48
Detection of Protein Ubiquitination in vitro
7:02
Representative Results
8:22
Conclusion
ユビキチン化は、3つの酵素のセットによって行わキー翻訳後修飾である。この変更に関与する遺伝子の変異が様々なヒト疾患に関連付けられています。ここで、我々は培養細胞でのタンパク質のユビキチン化を検出するためのプロトコルを説明 in vivoでと試験管 in vitroで。
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