Name: recordings of neural circuit activation in freely behaving animals
Uploaded: 2009-07-22T17:00:00
Description: Watch this Scientific Journal Video about Recordings of Neural Circuit Activation in Freely Behaving Animals at JoVE.com

洗澡的录音技术最早是由皮尔斯（1984）8和北奥等。 （1990）9，测量尾翻转时产生的电场。该技术后来又修改和完善唐纳德爱德华兹（美国乔治亚州立大学）博士在他的前研究生博士发地答：伊萨和他的前博士后副延Herberholz博士的实验室。已作出进一步的改进，并在马里兰大学的新研究中的应用已经在实验室的博士延Herberholz的测试。我想感谢给我帮助与实验中使用他的高速视频系统和我的研究助理大卫Rotstein和威廉利登，我的同事大卫耶格尔博士。

第1部分：录音室<ol><li>录音室是长方形的薄壁玻璃。商会尺寸为8.5厘米× 2厘米× 5厘米（长x宽x高）动物为2.5 - 3.5厘米（从主席台到尾节）总长度。 见图。例如在我们的实验中使用的一腔1。 </li><li>或者可以从其他材料（例如，无毒的透明塑料），录音室。钱伯斯的大小可能会有所不同根据实验过程和商会应为每一个实验系列定制。为了达到最佳效果，室尺寸应尽可能小，而不限制在他们的?...

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	<li>Herberholz, J., Issa, F. A., Edwards, D. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Patterns+of+neural+circuit+activation+and+behavior+during+dominance+hierarchy+formation+in+freely+behaving+crayfish.">Patterns of neural circuit activation and behavior during dominance hierarchy formation in freely behaving crayfish.</a> J. Neurosci. 21, 2759-2767 (2001).</li><li>Herberholz, J., Sen, M. M., Edwards, D. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Escape+behavior+and+escape+circuit+activation+in+juvenile+crayfish+during+prey-predator+interactions.">Escape behavior and escape circuit activation in juvenile crayfish during prey-predator interactions.</a> J. Exp. Biol. 207, 1855-1863 (2004).</li><li>Liden, W. H., Herberholz, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Behavioral+and+neural+responses+of+juvenile+crayfish+to+moving+shadows.J.">Behavioral and neural responses of juvenile crayfish to moving shadows.J.</a> Exp. Biol. 211, 1355-1361 (2008).</li><li>Finley, L. A., Macmillan, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+analysis+of+field+potentials+during+different+tailflip+behaviours+in+crayfish.">An analysis of field potentials during different tailflip behaviours in crayfish.</a> Mar. Freshw. Behav. Physiol. 35, 221-234 (2002).</li><li>Eaton, R. C., Lee, R. K. K., Foreman, M. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Mauthner+cell+and+other+identified+neurons+of+the+brainstem+escape+network+of+fish.">The Mauthner cell and other identified neurons of the brainstem escape network of fish.</a> Prog. Neurobiol. 63, 467-485 (2001).</li><li>Canfield, J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Some+voluntary+C-bends+may+be+Mauthner+neuron+initiated.">Some voluntary C-bends may be Mauthner neuron initiated.</a> J. Comp. Physiol. A. 193, 1055-1064 (2007).</li><li>W&#246;hl, S., Schuster, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+predictive+start+of+hunting+archer+fish:+a+flexible+and+precise+motor+pattern+performed+with+the+kinematics+of+an+escape+C-start.">The predictive start of hunting archer fish: a flexible and precise motor pattern performed with the kinematics of an escape C-start.</a> J. Exp. Biol. 210, 311-324 (2007).</li><li>Fricke, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+habituation+in+the+crayfish+due+to+selective+weakening+of+electrical+synapses.">Development of habituation in the crayfish due to selective weakening of electrical synapses.</a> Brain Res. 322, 139-143 (1984).</li><li>Beall, S. P., Langley, D. J., Edwards, D. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inhibition+of+escape+tailflip+in+crayfish+during+backward+walking+and+the+defense+posture.">Inhibition of escape tailflip in crayfish during backward walking and the defense posture.</a> J. Exp. Biol. 152, 577-582 (1990).</li></ol>

非侵入性的录音，单一的神经元活动或神经回路活化很难获得，奔放的动物。这里描述的方法提供了一种手段，以确定神经激活模式，基本自然发生的行为。 在过去，我们成功地使用这种技术来衡量少年小龙虾的神经逃脱电路的活动模式，在社会地位的层次1形成，在天敌2，最近的攻击，在 3种可视化的威胁。目前，我们使用同步的浴室电极录音和录?...

recordings of neural circuit activation in freely behaving animals

奔放的动物的神经活动和相应的行为表现模式之间的关系是很难建立。传统的非侵入性的方法需要至少部分内敛的研究课题，他们只允许同时激活的神经元大量的鉴定。另一方面，小合奏的神经元或个人神经元只能使用从很大程度上减少了筹备工作取得的单细胞记录。由于自然行为表达的是克制和解剖动物有限，基本控制这种行为的神经机制是难以辨认。 在这里，我提出了一种非侵入性的生理的技术，使测量在自由地表现动物的神经回路的激活。使用一对电极丝内充水室，洗澡的电极记录神经和肌肉的领域产生的少年小龙虾在自然的或实验诱发逃避反应的潜力。小龙虾的主要逃生反应介导的三个不同类型的尾翻转移动点刺激的动物。每种类型的尾巴翻转控制自己的神经回路;最快和最强大的的两个逃生反应需要两套不同的大型命令神经元的激活。浴电极录音与行为观察相结合，可以明确识别这些神经元和相关的神经回路。因此自然发生的行为背后的神经电路的活动可以测量奔放的动物，在不同的行为中。

Watch this Scientific Journal Video about Recordings of Neural Circuit Activation in Freely Behaving Animals at JoVE.com

Recordings of Neural Circuit Activation in Freely Behaving Animals

在自由的行为的动物的神经活动模式的非侵入性测量是通过结合神经生理学的高速录像录音。

自由表现动物的神经回路活化的录音

The relationship between patterns of neural activity and corresponding behavioral expression is difficult to establish in unrestrained animals. ...

Research

JoVE Journal

Biology

Whole Cell Recordings from Brain of Adult Drosophila

从整个成年果蝇大脑细胞记录

In this video, we demonstrate the procedure for isolating whole brains from adult Drosophila in preparation for recording from single neurons. We begin by ...

科研

生物学

Whole-cell Recordings of Light Evoked Excitatory Synaptic Currents in the Retinal Slice

全细胞记录的光在视网膜切片诱发兴奋性突触电流

We use the whole-cell patch clamp technique to study the synaptic circuitry that underlies visual information processing in the retina. In this video, we ...

Assay for Neural Induction in the Chick Embryo

在鸡胚神经诱导的含量为

The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ...

Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging

Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging

小动物的肌肉植入动态跟踪在体内荧光成像

Muscular dystrophies are a group of degenerative muscle diseases characterized by progressive loss of contractile muscle cells. Currently, there is no ...

Measures of Heart and Ventilatory Rates in Freely Moving Crayfish

在自由移动的小龙虾的心脏和换气率的措施

The fear, flight or fight response serves as the fundamental physiological basis for examining an organism's awareness of its environment under an ...

Single-cell Suction Recordings from Mouse Cone Photoreceptors

单从小鼠锥感光细胞吸录音

Rod and cone photoreceptors in the retina are responsible for light detection. In darkness, cyclic nucleotide-gated (CNG) channels in the outer segment ...

RhoC GTPase Activation Assay

RhoC的GTP酶激活法

RhoC GTPase has 91% homology to RhoA GTPase.  Because of its prevalence in cells, many reagents and techniques for RhoA GTPase have been developed.  ...

Modeling Biological Membranes with Circuit Boards and Measuring Electrical Signals in Axons: Student Laboratory Exercises

建模生物膜与电路板，并在轴突测量电气信号：学生实验室练习

This is a demonstration of how electrical models can be used to characterize biological membranes. This exercise also introduces biophysical terminology ...

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

分离和<em在体外激活线虫精子

Males and hermaphrodites are the two naturally found sexual forms in the nematode C. elegans. The amoeboid sperm are produced by both males and ...

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

激活细胞凋亡的细胞色素的细胞质显微注射 C</em

Apoptosis, or programmed cell death, is a conserved and highly regulated pathway by which cells die1.  Apoptosis can be triggered when cells encounter a ...