Name: preparation of rat brain aggregate cultures for neuron and glia development studies
Uploaded: 2009-09-30T23:59:00
Description: Watch this Scientific Journal Video about Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies at JoVE.com

这项研究是由部分启动资金，从得克萨斯A＆M大学

前夹层的制备 手术工具：由高压灭菌消毒清扫剪刀和镊子 盖玻片清洁： <ol><li>在1L草烧杯中33％的盐酸中浸泡至少24小时的所有盖玻片（直径为24孔板15毫米） </li><li>偶尔晃动的水10分钟，清除所有残留的盐酸洗</li><li>用微孔水冲洗</li><li>排水关闭</li><li>在95-98％的乙醇浸泡盖玻片</li><li>用干净的纸巾将盖玻片上平板和空气干燥的盖玻片</li><l...

<ol>
	<li>Matthieu, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myelination+in+rat+brain+aggregating+cell+cultures.">Myelination in rat brain aggregating cell cultures.</a> Neuroscience. 3, 565-56 (1978).</li><li>Chen, Y. i. n. g. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+and+culture+of+rat+and+mouse+oligodendrocyte+precursor+cells.">Isolation and culture of rat and mouse oligodendrocyte precursor cells.</a> Nat. Protocols. 2, 1044-10 (2007).</li><li>Watkins, T. A., Emery, B., Mulinyawe, S., Barres, B. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Distinct+stages+of+myelination+regulated+by+gamma-secretase+and+astrocytes+in+a+rapidly+myelinating+CNS+coculture+system.">Distinct stages of myelination regulated by gamma-secretase and astrocytes in a rapidly myelinating CNS coculture system.</a> Neuron. 60, 555-55 (2008).</li><li>Svenningsen, A. F., Shan, W. e. i. -. S. o. n. g., Colman, D. a. v. i. d. R., Pedraza, L. i. l. i. a. n. a. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapid+method+for+culturing+embryonic+neuron-glial+cell+cocultures.">Rapid method for culturing embryonic neuron-glial cell cocultures.</a> Journal of Neuroscience Research. 72, 565-56 (2003).</li></ol>

早期的研究报告突触介导的转自由浮动总文化的成熟髓鞘的形成。这里描述的中枢神经系统的总文化系统在三个维聚集，与传统的2D文化便利，以促进细胞的发育，迁移和体外分化的分析相结合的无血清多能祖细胞的生长，可以修改系统和神经前体细胞神经胶质细胞相互作用的调查研究和。例如，如少突胶质祖细胞的转基因细胞5月2日被添加到总文化。如小胶质细胞和巨噬细胞或神?...

preparation of rat brain aggregate cultures for neuron and glia development studies

在体外系统概括了发展和祖细胞分化为成熟的神经元和神经胶质细胞在中枢神经系统（CNS）的，将提供一个强大的神经学家平台，调查轴突胶质细胞相互作用，性能和多能祖细胞的分化，少突胶质细胞的进展在细胞和分子水平的细胞系细胞。我们在这里介绍从胚胎大鼠forebrains，在无血清培养基可维持3-4周，并在我们的实验室用来作为模型来研究神经元的神经胶质细胞的相互作用和中枢神经系统的髓鞘的中枢神经系统的总的培养体系。本短片将展示如何隔离和成长E16大鼠脑组织中的中枢神经系统的总文化。此外，从相同的大脑解剖，经常游离神经文化高度浓缩，可随时获得和使用各种研究，对中枢神经系统神经元或用于与其他细胞共培养。

Watch this Scientific Journal Video about Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies at JoVE.com

Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies

胚胎大鼠脑组织总文化系统的一个协议描述。在聚集的多能祖细胞可以发展和分化成神经元，星形胶质细胞和少突胶质细胞。

制备大鼠脑组织神经元和神经胶质发展研究总结文化

An in vitro system that recapitulates the development and differentiation of progenitors into mature neurons and glia in the central nervous system (CNS) ...

Research

JoVE Journal

Biology

Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae

从晚期果蝇蛹的大脑原发性神经文化

In this video, we demonstrate the preparation of primary neuronal cultures from the brains of late stage Drosophila pupae. The procedure begins with the ...

科研

生物学

Preparation of Neuronal Cultures from Midgastrula Stage Drosophila Embryos

从Midgastrula舞台果蝇胚胎神经文化的制备

This video illustrates the procedure for making primary neuronal cultures from midgastrula stage Drosophila embryos. The methods for collecting embryos ...

ALS - Motor Neuron Disease: Mechanism and Development of New Therapies

ALS - 运动神经元疾病：机制和新疗法的发展

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons

条块索玛的神经元和轴突的一个微流体设备制造

In this video, we demonstrate the technique of soft lithography with polydimethyl siloxane (PDMS) which we use to fabricate a microfluidic device for ...

Preparation of Dissociated Mouse Cortical Neuron Cultures

游离的小鼠皮层神经元文化的制备

This video will guide you through the process for generating cortical neuronal cultures from late embryo and early postnatal mouse brain. These cultures ...

Preparation of 2-dGuo-Treated Thymus Organ Cultures

2 dGuo治疗胸腺器官培养的制备

In the thymus, interactions between developing T-cell precursors and stromal cells that include cortical and medullary epithelial cells are known to play ...

Preparation of Aplysia Sensory-motor Neuronal Cell Cultures

Preparation of Aplysia Sensory-motor Neuronal Cell Cultures

制备海兔感觉运动神经元细胞培养

The nervous system of the marine mollusk Aplysia californica is relatively simple, consisting of approximately 20,000 neurons.  The neurons are large (up ...

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

一个多室中枢神经系统的神经胶质细胞共培养的微流体平台

We present a novel multi-compartment neuron co-culture microsystem platform for in vitro CNS axon-glia interaction research, capable of conducting up to ...

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

毒素诱导和蛋白质提取镰刀 SPP。</em蛋白质组学研究>文化

Fusaria are filamentous fungi able to produce different toxins. Fusarium mycotoxins such as deoxynivalenol, nivalenol, T2, zearelenone, fusaric acid, ...

Preparation of Rat Tail Tendons for Biomechanical and Mechanobiological Studies

制备鼠尾肌腱的生物力学和Mechanobiological研究

Rat tail tendons (RTTs) are a common biological model used in experimental in vitro studies in the fields of tendon physiology and tendinopathy. Working ...