Name: gross dissection of the stomach of the lobster, homarus americanus
Uploaded: 2009-05-22T00:00:00
Description: Watch this Scientific Journal Video about Gross Dissection of the Stomach of the Lobster, Homarus Americanus at JoVE.com

我们要感谢我们的顾问，博士除夕黄鼠狼。这项研究是由国家卫生赠款NS17813前夕黄鼠狼和NS059255研究所的支持曝光T。

<ol><li>解剖龙虾之前应埋在冰30分钟，麻醉动物。 </li><li>龙虾的性别可以看的第一个游泳足的形状决定的。的男性boney游泳和女性有羽毛状的游泳。 <img src="/files/ftp_upload/1320/1320fig1.png" alt="" /></li><li>隔离头胸部。 <ol><li>使用扭转运​​动撕掉前爪。 </li><li>取出使用大剪刀头胸部和腹部之间的深切割的腹部（尾部）。这削减巨大的纤维束，控制尾部翻转反应。然后进行切断腹部。 </li><li>拆下天线，他们用剪刀修?...

<ol>
	<li>Marder, E., Bucher, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Central+pattern+generators+and+the+control+of+rhythmic+movements.">Central pattern generators and the control of rhythmic movements.</a> Curr. Biol. 11, R986-R996 (2001).</li><li>Nusbaum, M. P., Beenhakker, M. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+small-systems+approach+to+motor+pattern+generation.">A small-systems approach to motor pattern generation.</a> Nature. 417, 343-350 (2002).</li><li>Clemens, S., Massabuau, J. C., Legeay, A., Meyrand, P., Simmers, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+modulation+of+interacting+central+pattern+generators+in+lobster+stomatogastric+ganglion:+influence+of+feeding+and+partial+pressure+of+oxygen.">In vivo modulation of interacting central pattern generators in lobster stomatogastric ganglion: influence of feeding and partial pressure of oxygen.</a> J. Neurosci. 18, 2788-2799 (1998).</li><li>Tobin, A. E., Bierman, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Homarus+americanus+stomatogastric+nervous+system+dissection.">Homarus americanus stomatogastric nervous system dissection.</a> J Vis Exp. 27, (2009).</li><li>Maynard, D. M., Dando, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+structure+of+the+stomatogastric+neuromuscular+system+in+Callinectes+sapidus,+Homarus+Americanus+and+Panulirus+argus+(Decapoda+crustacean).">The structure of the stomatogastric neuromuscular system in Callinectes sapidus, Homarus Americanus and Panulirus argus (Decapoda crustacean).</a> Philos. Trans. R. Soc. Lond. B Biol. Sci. 268, 161-220 (1974).</li><li>Herrick, F. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Natural+history+of+the+American+Lobster.">Natural history of the American Lobster.</a> U.S. Bur. Fish. , 219-252 (1909).</li><li>Marder, E., Bucher, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Understanding+circuit+dynamics+using+the+stomatogastric+nervous+system+of+lobsters+and+crabs.">Understanding circuit dynamics using the stomatogastric nervous system of lobsters and crabs.</a> Annu. Rev. Physiol. 69, 291-316 (2007).</li></ol>

在我们的龙虾胃清扫实验室做访问stomatogastric的神经系统，网络控制胃的节律性收缩。龙虾胃的解剖结构已经得到了很好的描述以前5-7，我们建议变得熟悉其解剖。这样做会破坏​​脆弱的神经和肌肉的风险降低。 胃清扫完成后，各个部分的STNS可能进一步解剖。例如，研究神经肌肉交界处，STNS神经，它支配的肌肉可能会被孤立。在stomatogastric神经节神经元的somata可以...

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		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>Homarus americanus</td>
<td>&nbsp;</td>
<td>Commercial Lobster</td>
<td>&nbsp;</td>
<td>Can be purchased from any lobster supplier, We specifically request &#x201C;chicken&#x201D; lobsters.</td>
</tr>
<tr>
<td>Black Sylgard 170</td>
<td>&nbsp;</td>
<td>Dow Corning</td>
<td>170</td>
<td>&nbsp;</td>
<tr>
<td>Preparation Dish</td>
<td>&nbsp;</td>
<td>Fischer </td>
<td>08-807</td>
<td>Fill 2" with Black Sylgard 170.</td>
</tr>
<tr>
<td>Fine Scissors</td>
<td>&nbsp;</td>
<td>FST</td>
<td>14090-09</td>
<td>&nbsp;</td>
<tr>
<td>Insect Pins size 6</td>
<td>&nbsp;</td>
<td>FST</td>
<td>26000-65</td>
<td>&nbsp;</td>
<tr>
<td>Mayo Scissors</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>This is a common product offered by many suppliers of dissecting tools.</td>
</tr>
<tr>
<td>Micro-Spatula</td>
<td>&nbsp;</td>
<td>Ward Sci.</td>
<td>15 V 4313</td>
<td>This is a very common product offered by many suppliers of lab tools.</td>
</tr>
<tr>
<td>Rongeurs</td>
<td>&nbsp;</td>
<td>FST</td>
<td>&nbsp;</td>
<td>16000-14</td>
</tr>
<tr>
<td>Plastic hemostat</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>This is a common product offered by many suppliers of dissecting or jewelry making tools.</td>
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gross dissection of the stomach of the lobster, homarus americanus

美国龙虾的胃（<i&gt; Homarus americanus</i&gt;）位于头胸部之间的讲台上和颈沟。张口后部和底部的幽门是指胃前壁。随着胃背侧在于stomatogastric中枢神经系统（STNS）。这种神经系统，其中包含有节奏的网络，摄食行为的背后，是一个建立的模型系统研究节奏产生网络和神经调控<sup&gt; 1,2</sup&gt;。虽然有可能为研究本系统<i&gt;在体内</i<sup&gt; 3</sup，STNS继续隔离时产生的节律活动<i&gt;在体外</i&gt;。为了研究这个系统<i&gt;在体外</i&gt;胃，必须从动物中删除。此视频文章介绍了如何可以从美国龙虾胃解剖。在随后的视频文章<sup&gt; 4</sup&gt;我们展示了如何ST​​NS可以从胃隔离。

Watch this Scientific Journal Video about Gross Dissection of the Stomach of the Lobster, Homarus Americanus at JoVE.com

Gross Dissection of the Stomach of the Lobster, Homarus Americanus

我们描述了美国龙虾的胃总值清扫（<i&gt; Homarus americanus</i&gt;）。

肚子里的龙虾总夹层， Homarus Americanus</em

The stomach of the American lobster (Homarus americanus) is located in the cephalothorax, between the rostrum and the cervical groove. The anterior end of ...

Research

JoVE Journal

Biology

Gross Dissection of the Stomach of the Lobster, Homarus Americanus

Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region

从特定的大脑区域的RNA或蛋白质提取大鼠脑微解剖

Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates ...

科研

生物学

Homarus Americanus Stomatogastric Nervous System Dissection

Homarus Americanus Stomatogastric Nervous System Dissection

Homarus Americanus Stomatogastric神经系统解剖

With the goal of understanding how nervous systems produce activity and respond to the environment, neuroscientists turn to model systems that exhibit the ...

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Gross and Fine Dissection of Inner Ear Sensory Epithelia in Adult Zebrafish Danio rerio

格罗斯和精细解剖内耳感觉上皮细胞在成年斑马鱼（斑马鱼）

Neurosensory epithelia in the inner ear are the crucial structures for hearing and balance functions. Therefore, it is important to understand the ...

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使用范围，以确定潜在的监管图案协同调控基因

SCOPE is an ensemble motif finder that uses three component algorithms in parallel to identify potential regulatory motifs by over-representation and ...

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成年斑马鱼肾脏的解剖

Researchers working in the burgeoning field of adult stem cell biology seek to understand the signals that regulate the behavior and function of stem ...

Dissection and Flat-mounting of the Threespine Stickleback Branchial Skeleton

解剖和三刺鳃骨架的平面安装

The posterior pharyngeal segments of the vertebrate head give rise to the branchial skeleton, the primary site of food processing in fish. The morphology ...

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

在出生后的小鼠听泡解剖：中耳骨骼和组织学分析的分离

In most mammals, auditory ossicles in the middle ear, including the malleus, incus and stapes, are the smallest bones. In mice, a bony structure called ...

Reverse Dissection and DiceCT Reveal Otherwise Hidden Data in the Evolution of the Primate Face

灵长类动物脸演变过程中的反向解剖与 dicect 显示其他隐藏数据

Facial expressions, or facial displays, of social or emotional intent are produced by many mammalian taxa as a means of visually communicating with ...

Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae

Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae

果蝇幼虫的欧诺细胞的解剖和脂滴染色

Lipids are essential for animal development and physiological homeostasis. Dysregulation of lipid metabolism results in various developmental defects and ...