Name: gross dissection of the stomach of the lobster, homarus americanus
Uploaded: 2009-05-22T00:00:00
Description: Watch this Scientific Journal Video about Gross Dissection of the Stomach of the Lobster, Homarus Americanus at JoVE.com

Vogliamo ringraziare il nostro consulente, il dottor Eve Marder. Questa ricerca è stata finanziata dal National Institute of concede Salute NS17813 a Eve Marder e NS059255 di AE T.

<ol><li> Prima della dissezione l&#39;aragosta deve essere sepolto nel ghiaccio per 30 minuti per anestetizzare l&#39;animale. </li><li> Il sesso del aragosta può essere determinato osservando la forma della swimmeret prima. I maschi hanno swimmerets boney e femmine hanno swimmerets pennuto. <img src="/files/ftp_upload/1320/1320fig1.png" alt="" /></li><li> Isolare il cefalotorace. <ol><li> Utilizzare un movimento rotatorio per strappare gli artigli anteriori. </li><li> Rimuovere l&#39;addome (parte posteriore) utilizzando le forbici gran...

<ol>
	<li>Marder, E., Bucher, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Central+pattern+generators+and+the+control+of+rhythmic+movements.">Central pattern generators and the control of rhythmic movements.</a> Curr. Biol. 11, R986-R996 (2001).</li><li>Nusbaum, M. P., Beenhakker, M. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+small-systems+approach+to+motor+pattern+generation.">A small-systems approach to motor pattern generation.</a> Nature. 417, 343-350 (2002).</li><li>Clemens, S., Massabuau, J. C., Legeay, A., Meyrand, P., Simmers, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+modulation+of+interacting+central+pattern+generators+in+lobster+stomatogastric+ganglion:+influence+of+feeding+and+partial+pressure+of+oxygen.">In vivo modulation of interacting central pattern generators in lobster stomatogastric ganglion: influence of feeding and partial pressure of oxygen.</a> J. Neurosci. 18, 2788-2799 (1998).</li><li>Tobin, A. E., Bierman, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Homarus+americanus+stomatogastric+nervous+system+dissection.">Homarus americanus stomatogastric nervous system dissection.</a> J Vis Exp. 27, (2009).</li><li>Maynard, D. M., Dando, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+structure+of+the+stomatogastric+neuromuscular+system+in+Callinectes+sapidus,+Homarus+Americanus+and+Panulirus+argus+(Decapoda+crustacean).">The structure of the stomatogastric neuromuscular system in Callinectes sapidus, Homarus Americanus and Panulirus argus (Decapoda crustacean).</a> Philos. Trans. R. Soc. Lond. B Biol. Sci. 268, 161-220 (1974).</li><li>Herrick, F. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Natural+history+of+the+American+Lobster.">Natural history of the American Lobster.</a> U.S. Bur. Fish. , 219-252 (1909).</li><li>Marder, E., Bucher, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Understanding+circuit+dynamics+using+the+stomatogastric+nervous+system+of+lobsters+and+crabs.">Understanding circuit dynamics using the stomatogastric nervous system of lobsters and crabs.</a> Annu. Rev. Physiol. 69, 291-316 (2007).</li></ol>

Nel nostro laboratorio di dissezione dello stomaco aragosta viene fatto per accedere al sistema stomatogastric nervoso, una rete che controlla le contrazioni ritmiche dello stomaco. L&#39;anatomia dello stomaco aragosta è stata ben descritta in precedenza 5-7, e si consiglia di familiarizzare con la sua anatomia. In questo modo si riduce il rischio di danneggiare i nervi delicati e muscolare. Al termine della dissezione stomaco, varie parti del STNS possono essere ulteriormente s...

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		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>Homarus americanus</td>
<td>&nbsp;</td>
<td>Commercial Lobster</td>
<td>&nbsp;</td>
<td>Can be purchased from any lobster supplier, We specifically request &#x201C;chicken&#x201D; lobsters.</td>
</tr>
<tr>
<td>Black Sylgard 170</td>
<td>&nbsp;</td>
<td>Dow Corning</td>
<td>170</td>
<td>&nbsp;</td>
<tr>
<td>Preparation Dish</td>
<td>&nbsp;</td>
<td>Fischer </td>
<td>08-807</td>
<td>Fill 2" with Black Sylgard 170.</td>
</tr>
<tr>
<td>Fine Scissors</td>
<td>&nbsp;</td>
<td>FST</td>
<td>14090-09</td>
<td>&nbsp;</td>
<tr>
<td>Insect Pins size 6</td>
<td>&nbsp;</td>
<td>FST</td>
<td>26000-65</td>
<td>&nbsp;</td>
<tr>
<td>Mayo Scissors</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>This is a common product offered by many suppliers of dissecting tools.</td>
</tr>
<tr>
<td>Micro-Spatula</td>
<td>&nbsp;</td>
<td>Ward Sci.</td>
<td>15 V 4313</td>
<td>This is a very common product offered by many suppliers of lab tools.</td>
</tr>
<tr>
<td>Rongeurs</td>
<td>&nbsp;</td>
<td>FST</td>
<td>&nbsp;</td>
<td>16000-14</td>
</tr>
<tr>
<td>Plastic hemostat</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>This is a common product offered by many suppliers of dissecting or jewelry making tools.</td>
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gross dissection of the stomach of the lobster, homarus americanus

Lo stomaco della aragosta americana (<i&gt; Homarus americanus</i&gt;) Si trova nel cefalotorace, tra la tribuna e il solco cervicale. L&#39;estremità anteriore dello stomaco è definito dalla apertura della bocca e l&#39;estremità posteriore del fondo del piloro. Lungo il lato dorsale dello stomaco si trova il sistema nervoso stomatogastric (STNS). Questo sistema nervoso, che contiene le reti che sono alla base ritmica comportamento alimentare, è un sistema modello per studiare le reti stabilito ritmo generazione e neuromodulazione<sup&gt; 1,2</sup&gt;. Mentre è possibile studiare questo sistema<i&gt; In vivo</i<sup&gt; 3</sup&gt;, Il STNS continua a produrre la sua attività ritmica quando isolato<i&gt; In vitro</i&gt;. Per studiare questo sistema<i&gt; In vitro</i&gt; Lo stomaco deve essere rimosso dall&#39;animale. In questo articolo video descrive come lo stomaco può essere sezionato dalla aragosta americana. In un articolo che accompagna il video<sup&gt; 4</sup&gt; Ci dimostrano come la STNS può essere isolato dallo stomaco.

Watch this Scientific Journal Video about Gross Dissection of the Stomach of the Lobster, Homarus Americanus at JoVE.com

Gross Dissection of the Stomach of the Lobster, Homarus Americanus

Descriviamo la dissezione lordo dello stomaco del aragosta americana (<i&gt; Homarus americanus</i&gt;).

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The stomach of the American lobster (Homarus americanus) is located in the cephalothorax, between the rostrum and the cervical groove. The anterior end of ...

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Gross Dissection of the Stomach of the Lobster, Homarus Americanus

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