Name: gross dissection of the stomach of the lobster, homarus americanus
Uploaded: 2009-05-22T00:00:00
Description: Watch this Scientific Journal Video about Gross Dissection of the Stomach of the Lobster, Homarus Americanus at JoVE.com

Vi skulle vilja att erkänna våra rådgivare, Dr Eva Marder. Denna forskning stöds av National Institute of Health bidrag NS17813 för Eva Marder och NS059255 till AE T.

<ol><li> Före dissektion hummer bör bli begravd i is i 30 minuter att söva djuret. </li><li> I kön hummer kan bestämmas genom att titta på formen på den första swimmeret. Hanar har Boney swimmerets och kvinnor har fjäderlätt swimmerets. <img src="/files/ftp_upload/1320/1320fig1.png" alt="" /></li><li> Isolera cephalothorax. <ol><li> Använd en vridande rörelse för att slita av de främre klorna. </li><li> Ta bort buken (svansen delen) med hjälp av stora saxar för att göra ett djupt snitt mellan de cephalothorax och buken. D...

<ol>
	<li>Marder, E., Bucher, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Central+pattern+generators+and+the+control+of+rhythmic+movements.">Central pattern generators and the control of rhythmic movements.</a> Curr. Biol. 11, R986-R996 (2001).</li><li>Nusbaum, M. P., Beenhakker, M. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+small-systems+approach+to+motor+pattern+generation.">A small-systems approach to motor pattern generation.</a> Nature. 417, 343-350 (2002).</li><li>Clemens, S., Massabuau, J. C., Legeay, A., Meyrand, P., Simmers, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+modulation+of+interacting+central+pattern+generators+in+lobster+stomatogastric+ganglion:+influence+of+feeding+and+partial+pressure+of+oxygen.">In vivo modulation of interacting central pattern generators in lobster stomatogastric ganglion: influence of feeding and partial pressure of oxygen.</a> J. Neurosci. 18, 2788-2799 (1998).</li><li>Tobin, A. E., Bierman, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Homarus+americanus+stomatogastric+nervous+system+dissection.">Homarus americanus stomatogastric nervous system dissection.</a> J Vis Exp. 27, (2009).</li><li>Maynard, D. M., Dando, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+structure+of+the+stomatogastric+neuromuscular+system+in+Callinectes+sapidus,+Homarus+Americanus+and+Panulirus+argus+(Decapoda+crustacean).">The structure of the stomatogastric neuromuscular system in Callinectes sapidus, Homarus Americanus and Panulirus argus (Decapoda crustacean).</a> Philos. Trans. R. Soc. Lond. B Biol. Sci. 268, 161-220 (1974).</li><li>Herrick, F. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Natural+history+of+the+American+Lobster.">Natural history of the American Lobster.</a> U.S. Bur. Fish. , 219-252 (1909).</li><li>Marder, E., Bucher, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Understanding+circuit+dynamics+using+the+stomatogastric+nervous+system+of+lobsters+and+crabs.">Understanding circuit dynamics using the stomatogastric nervous system of lobsters and crabs.</a> Annu. Rev. Physiol. 69, 291-316 (2007).</li></ol>

I vårt laboratorium dissekering av hummer magen görs för att få tillgång till stomatogastric nervsystemet, ett nätverk som styr rytmiska sammandragningar i magen. Anatomi av hummer magen har blivit väl beskriven tidigare 5-7, och vi rekommenderar att bli bekant med dess anatomi. Om du gör det kommer att minska risken för skador på känsliga nerver och muskler. Efter avslutad magen dissektion kan olika delar av STNS ytterligare dissekeras. Till exempel för att studera ne...

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		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>Homarus americanus</td>
<td>&nbsp;</td>
<td>Commercial Lobster</td>
<td>&nbsp;</td>
<td>Can be purchased from any lobster supplier, We specifically request &#x201C;chicken&#x201D; lobsters.</td>
</tr>
<tr>
<td>Black Sylgard 170</td>
<td>&nbsp;</td>
<td>Dow Corning</td>
<td>170</td>
<td>&nbsp;</td>
<tr>
<td>Preparation Dish</td>
<td>&nbsp;</td>
<td>Fischer </td>
<td>08-807</td>
<td>Fill 2" with Black Sylgard 170.</td>
</tr>
<tr>
<td>Fine Scissors</td>
<td>&nbsp;</td>
<td>FST</td>
<td>14090-09</td>
<td>&nbsp;</td>
<tr>
<td>Insect Pins size 6</td>
<td>&nbsp;</td>
<td>FST</td>
<td>26000-65</td>
<td>&nbsp;</td>
<tr>
<td>Mayo Scissors</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>This is a common product offered by many suppliers of dissecting tools.</td>
</tr>
<tr>
<td>Micro-Spatula</td>
<td>&nbsp;</td>
<td>Ward Sci.</td>
<td>15 V 4313</td>
<td>This is a very common product offered by many suppliers of lab tools.</td>
</tr>
<tr>
<td>Rongeurs</td>
<td>&nbsp;</td>
<td>FST</td>
<td>&nbsp;</td>
<td>16000-14</td>
</tr>
<tr>
<td>Plastic hemostat</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>This is a common product offered by many suppliers of dissecting or jewelry making tools.</td>
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gross dissection of the stomach of the lobster, homarus americanus

Magen av den amerikanska hummern (<i&gt; Homarus americanus</i&gt;) Ligger i cephalothorax, mellan talarstolen och livmoderhalscancer spåret. Den främre änden av magen definieras av munnen öppnas och den bakre änden av botten av pylorus. Längs ryggens sidan av magen ligger stomatogastric nervsystemet (STNS). Detta nervsystemet, som innehåller rytmiska nätverk som ligger bakom ätbeteende, är ett etablerat modellsystem för att studera rytm skapa nätverk och neuromodulering<sup&gt; 1,2</sup&gt;. Även om det är möjligt att studera detta system<i&gt; In vivo</i<sup&gt; 3</sup&gt; Fortsätter STNS att producera sin rytmiska aktivitet när isolerade<i&gt; In vitro</i&gt;. För att studera detta system<i&gt; In vitro</i&gt; Magen måste avlägsnas från djuret. Denna video artikeln beskrivs hur magen kan dissekeras från den amerikanska hummer. I en medföljande video artikeln<sup&gt; 4</sup&gt; Vi visa hur STNS kan isoleras från magen.

Watch this Scientific Journal Video about Gross Dissection of the Stomach of the Lobster, Homarus Americanus at JoVE.com

Gross Dissection of the Stomach of the Lobster, Homarus Americanus

Vi beskriver de grova dissektion i magen av den amerikanska hummern (<i&gt; Homarus americanus</i&gt;).

Gross Dissection i magen av Hummer, Homarus americanus</em

The stomach of the American lobster (Homarus americanus) is located in the cephalothorax, between the rostrum and the cervical groove. The anterior end of ...

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Biology

Gross Dissection of the Stomach of the Lobster, Homarus Americanus

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