Name: combining peripheral nerve grafting and matrix modulation to repair the injured rat spinal cord
Uploaded: 2009-11-20T17:00:00
Description: Watch this Scientific Journal Video about Combining Peripheral Nerve Grafting and Matrix Modulation to Repair the Injured Rat Spinal Cord at JoVE.com

<p class="jove_content">这项工作是由美国国立卫生研究院资助/ NINDS NS26380和NS55976，克里斯托弗和Dana里夫基金会和丹尼尔Heumann为脊髓研究基金的支持。德雷克塞尔大学医学院脊髓研究中心提供支持来完成这项工作的核心设施。 </p>

<p class="jove_title"> 1）显微手术的准备工作</p><ol><li>手术站需要用稀释的漂白解决方案之前，设置仪器和辅助材料，清洁和消毒。蒸压工具将1天手术前，在无菌容器中。打开热珠的灭菌器，用于去除从程序在不同的动物之间的文书的病原体和微生物污染物（精细科学的工具）。 </li><li>插入热障使用在手术过程中保持体温。设置用于控制出血的棉签和纱布。准备凝胶泡沫的小（小于<sup>2mm</sup> 3）微止血和无菌生理盐?...

<ol>
	<li>Houle, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Demonstration+of+the+potential+for+chronically+injured+neurons+to+regenerate+axons+into+intraspinal+peripheral+nerve+grafts.">Demonstration of the potential for chronically injured neurons to regenerate axons into intraspinal peripheral nerve grafts.</a> <em style='font-style: italic'>Exp. Neurol</em>. <b>113</b>, 1-9 (1991).</li><li>Ye, J. H., Houle, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Treatment+of+the+chronically+injured+spinal+cord+with+neurotrophic+factors+can+promote+axonal+regeneration+from+supraspinal+neurons.">Treatment of the chronically injured spinal cord with neurotrophic factors can promote axonal regeneration from supraspinal neurons.</a> <em style='font-style: italic'>Exp. Neurol</em>. <b>143</b>, 70-81 (1997).</li><li>Houle, J. D., Tom, V., Mayes, D., Wagoner, G., Phillips, N., Silver, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Combining+an+autologous+peripheral+nerve+bridge+and+matrix+modification+by+chondroitinase+allows+robust+functional+regeneration+across+a+hemisection+lesion+of+the+adult+rat+spinal+cord.">Combining an autologous peripheral nerve bridge and matrix modification by chondroitinase allows robust functional regeneration across a hemisection lesion of the adult rat spinal cord.</a> <em style='font-style: italic'>J. Neurosci</em>. <b>26</b>, 7405-7415 (2006).</li><li>Tom, V., Houle, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intraspinal+microinjection+of+chondroitinase+ABC+following+injury+promotes+axonal+regeneration+out+of+a+peripheral+nerve+graft.">Intraspinal microinjection of chondroitinase ABC following injury promotes axonal regeneration out of a peripheral nerve graft.</a> <em style='font-style: italic'>Exp. Neurol</em>. <b>211</b>, 315-319 (2008).</li></ol>

<ol><li>实验的可重复性，它是重要的，在动物脊髓损伤的水平是一致的。因此，至关重要的是，确定相应的椎椎板切除过程。因为我们使用的是这项研究的一个半切或切断病变模型，有没有病变的大小或含糊之处，具体脊髓束是否受伤或幸免。 </li><li>周围神经移植前预先堕落，因为这启动消化外围的轴突再生轴突生长提供空间，因为它会激活雪旺氏细胞产生多种神经营养因子支持轴突长入，这是必要的。 </li><li>初...

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		<th>Catalogue Number</th>
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<td>10-0 silk suture</td>
<td>&nbsp;</td>
<td>ArosSurgical</td>
<td>T5A10N10</td>
<td>&nbsp;</td>
<tr>
<td>6-0 silk suture</td>
<td>&nbsp;</td>
<td>McKesson</td>
<td>2693</td>
<td>&nbsp;</td>
<tr>
<td>Ampicillin</td>
<td>&nbsp;</td>
<td>McKesson</td>
<td>483549</td>
<td>&nbsp;</td>
<tr>
<td>Antibody to cFos</td>
<td>&nbsp;</td>
<td>Sigma-Aldrich</td>
<td>F7799</td>
<td>&nbsp;</td>
<tr>
<td>Biotinylated dextran Amine</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>D7135</td>
<td>&nbsp;</td>
<tr>
<td>Buprenorphin (.3mg/ml)</td>
<td>&nbsp;</td>
<td>McKesson</td>
<td>12496075701</td>
<td>&nbsp;</td>
<tr>
<td>Chondroitinase ABC</td>
<td>&nbsp;</td>
<td>Associates of Cape Cod</td>
<td>100332-1A</td>
<td>&nbsp;</td>
<tr>
<td>Euthasol</td>
<td>&nbsp;</td>
<td>Webster Veterinary</td>
<td>07-805-9296</td>
<td>&nbsp;</td>
<tr>
<td>Hanks Balanced Salt Solution</td>
<td>&nbsp;</td>
<td>Cellgro</td>
<td>21-021-CV</td>
<td>&nbsp;</td>
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<td>Isoflurane</td>
<td>&nbsp;</td>
<td>Henry Schein</td>
<td>209-1966</td>
<td>&nbsp;</td>
<tr>
<td>Michel Wound Clips</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>12040-02</td>
<td>&nbsp;</td>
<tr>
<td>Neurotrace Kit</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>N7167</td>
<td>&nbsp;</td>
<tr>
<td>True Blue</td>
<td>&nbsp;</td>
<td>Sigma-Aldrich</td>
<td>T5891</td>
<td>&nbsp;</td>
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<td>Xenodine</td>
<td>&nbsp;</td>
<td>Webster Veterinary</td>
<td>92201</td>
<td>&nbsp;</td>
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<td>Hot Bead Sterilizer</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>Forced Exercise Wheel</td>
<td>&nbsp;</td>
<td>Lafayette Instruments</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>TreadScan System</td>
<td>&nbsp;</td>
<td>Clever System</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Infinite Horizon Impact Device</td>
<td>&nbsp;</td>
<td>Precision Systems and Instrumentation</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Magnetic Stimulation Device</td>
<td>&nbsp;</td>
<td>MagStim Inc.</td>
<td>&nbsp;</td>
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combining peripheral nerve grafting and matrix modulation to repair the injured rat spinal cord

<p class="jove_content">外伤性脊髓损伤（SCI）的会导致死亡的神经元，运动和感觉神经纤维（轴突）与大脑的沟通途径和中断的中断。我们研究的目标之一是促进轴突再生，恢复整个病变部位的连接。而要做到这一点，我们制定了周围神经（PN）的坐骨神经段是直接放在之间的脊髓受损的结束或用于整个病变的桥梁形式嫁接技术。这种方法有几个优点比其他神经组织移植，再生轴突可以对一个具体的目标区域的指示，再生轴突的数量和源代码很容易被追踪技术确定，移植可用于电生理实验来衡量功能的恢复与轴突在移植，并有可能使用的自体神经，以减少移植排斥反应的可能性。在我们的实验室中，我们已经完成的自体（捐赠者和接受者是同一种动物）和异源（捐赠者和接受者是不同的动物）移植与比较的结果。这种方法已成功地用于急性和慢性损伤的情况下。再生轴突到达末端的PN移植往往不能延伸到脊髓背，所以我们使用的硫酸软骨素microinjections，降低与SCI的地区周围的疤痕组织相关抑制分子。与此同时，我们发现，提供外源性生长和营养分子，鼓励到距离较远的脊髓神经轴突再生。移植后的几个月，我们执行各种解剖，行为和电生理测试，以评估在脊髓受伤的动物功能的恢复。在不同程度的损伤，并在不同的物种，这种实验方法已成功地用于在几个脊髓损伤模型（小鼠，大鼠和猫）。重要的是，周围神经移植的方法是有效地促进急性和长期受伤的神经元的再生。</p>

Watch this Scientific Journal Video about Combining Peripheral Nerve Grafting and Matrix Modulation to Repair the Injured Rat Spinal Cord at JoVE.com

Combining Peripheral Nerve Grafting and Matrix Modulation to Repair the Injured Rat Spinal Cord

<p class="jove_content">脊髓外伤，会扰乱大脑的沟通。要恢复失去的连接，我们利用了周围神经移植提供一个底层与神经营养因子和基质调节酶，再生纤维结合，以消除抑制分子，促进长途增长。</p>

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Traumatic injury to the spinal cord (SCI) causes death of neurons, disruption of motor and sensory nerve fiber (axon) pathways and disruption of ...

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