Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants

DOI :

10.3791/134-v

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January 30th, 2007

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7,676 Views

D. Spencer Currle¹, Aaron Kolski-Andreaco², Edwin S. Monuki³

¹Department of Developmental and Cell Biology, University of California, Irvine (UCI), ²Department of Physiology and Biophysics, University of California, Irvine (UCI), ³Department of Pathology, University of California, Irvine (UCI)

Chapters

This video demonstrates two methods for preparing and placing beads, which have been coated with growth factor, on explants of the developing cerebral cortex. These beads can be used to induce spatially restricted gene expression on developing neural tissue such as forebrain explants. Methods are given for using both Affi-Gel beads and heparin acryllic beads.