Name: electrophysiological recording in the drosophila embryo
Uploaded: 2009-05-21T00:00:00
Description: Watch this Scientific Journal Video about Electrophysiological Recording in the Drosophila Embryo at JoVE.com

КБ поддерживается NIH грант GM54544.

Часть 1: Оборудование и расходные материалы <ol><li> Электрофизиологические записи из эмбрионов дрозофилы в первую очередь требует знания эмбриональных методы вскрытия, которые описаны в другой Юпитер видео. </li><li> Электрофизиологические записи из эмбрионов дрозофилы использу...

Электрофизиологические записи из эмбрионов дрозофилы требует ручного манипулирования и рассечение. Здоровье подготовки, и, как следствие качество записей, зависит от того, один из которых в состоянии быстро и аккуратно подготовить хрупкой эмбриональных тканей для записи, а зате?...

electrophysiological recording in the drosophila embryo

Drosophila is a premier genetic model for the study of both embryonic development and functional neuroscience. Traditionally, these fields are quite isolated from each other, with largely independent histories and scientific communities. However, the interface between these usually disparate fields is the developmental programs underlying acquisition of functional electrical signaling properties and differentiation of functional chemical synapses during the final phases of neural circuit formation. This interface is a critically important area for investigation. In Drosophila, these phases of functional development occur during a period of &lt;8 hours (at 25&deg;C) during the last third of embryogenesis. This late developmental period was long considered intractable to investigation owing to the deposition of a tough, impermeable epidermal cuticle. A breakthrough advance was the application of water-polymerizing surgical glue that can be locally applied to the cuticle to enable controlled dissection of late-stage embryos. With a dorsal longitudinal incision, the embryo can be laid flat, exposing the ventral nerve cord and body wall musculature to experimental investigation. Whole-cell patch-clamp techniques can then be employed to record from individually-identifiable neurons and somatic muscles. These recording configurations have been used to track the appearance and maturation of ionic currents and action potential propagation in both neurons and muscles. Genetic mutants affecting these electrical properties have been characterized to reveal the molecular composition of ion channels and associated signaling complexes, and to begin exploration of the molecular mechanisms of functional differentiation. A particular focus has been the assembly of synaptic connections, both in the central nervous system and periphery. The glutamatergic neuromuscular junction (NMJ) is most accessible to a combination of optical imaging and electrophysiological recording. A glass suction electrode is used to stimulate the peripheral nerve, with excitatory junction current (EJC) recordings made in the voltage-clamped muscle. This recording configuration has been used to chart the functional differentiation of the synapse, and track the appearance and maturation of presynaptic glutamate release properties. In addition, postsynaptic properties can be assayed independently via iontophoretic or pressure application of glutamate directly to the muscle surface, to measure the appearance and maturation of the glutamate receptor fields. Thus, both pre- and postsynaptic elements can be monitored separately or in combination during embryonic synaptogenesis. This system has been heavily used to isolate and characterize genetic mutants that impair embryonic synapse formation, and thus reveal the molecular mechanisms governing the specification and differentiation of synapse connections and functional synaptic signaling properties.

Watch this Scientific Journal Video about Electrophysiological Recording in the Drosophila Embryo at JoVE.com

Electrophysiological Recording in the Drosophila Embryo

Электрофизиологические записи с<em&gt; Drosophila</em&gt; Эмбрионов позволит анализ развития мышц и нейронов электрическим свойствам, а также характеристика функциональных синаптогенез на глутаматергической нервно-мышечном соединении и центральной холинергической и ГАМК-синапсы.

Электрофизиологические Запись в Drosophila Эмбрионов

Drosophila is a premier genetic model for the study of both embryonic development and functional neuroscience. Traditionally, these fields are quite ...

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JoVE Journal

Biology

Electrophysiological Recording in the Drosophila Embryo

Assay for Neural Induction in the Chick Embryo

Анализ для нейронных Индукция в куриных эмбрионах

The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ...

Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae

Электрофизиологические методы регистрации синаптические потенциалы от NMJ личинок дрозофилы

In this video, we describe the electrophysiological methods for recording synaptic transmission at the neuromuscular junction (NMJ) of Drosophila larva. ...

Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures

Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures

Сбор и подготовка Drosophila Эмбрионы для Электрофизиологические Запись и другие процедуры

Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo

Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo

Микроинъекция методы для изучения митоза в Дрозофилы Синцитиальный эмбрионов

This protocol describes the use of the Drosophila melanogaster syncytial embryo  for studying mitosis1.  Drosophila has useful genetics with a sequenced ...

Visualizing the Beating Heart in Drosophila

Visualizing the Beating Heart in Drosophila

Визуализация бьющееся сердце в Drosophila</em

The Drosophila heart has recently emerged as a good model system for examining the genetic, cellular, and molecular mechanisms underlying function in ...

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Подготовка эмбрионов для электронной микроскопии Drosophila Эмбриональные трубки сердца

The morphogenesis of the Drosophila  embryonic heart tube has emerged as a valuable model system for studying cell migration, cell-cell adhesion and cell ...

Ambulatory ECG Recording in Mice

Амбулаторно запись ЭКГ у мышей

Telemetric ECG recording in mice is essential to understanding the mechanisms behind arrhythmias, conduction disorders, and sudden cardiac death. Although ...

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

Оптимизированная стратегия Трансфекция для выражения и Электрофизиологические Запись рекомбинантных напряжением ионные каналы в НЕК-293T Клетки

The in vitro expression and electrophysiological recording of recombinant voltage-gated ion channels in cultured human embryonic kidney cells (HEK-293T) ...

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo

Отслеживание Деформации Морфогенетические тканей в раннем куриного эмбриона

Embryonic epithelia undergo complex deformations (e.g. bending, twisting, folding, and stretching) to form the primitive organs of the early embryo. ...

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

Живая съемка апоптоза клеток во время акции<em&gt; Drosophila</em&gt; Эмбриогенеза

The  proper elimination of unwanted or aberrant cells through apoptosis and  subsequent phagocytosis (apoptotic cell clearance) is crucial for normal  ...