Name: using the horseshoe crab, limulus polyphemus, in vision research
Uploaded: 2009-07-03T00:00:00
Description: Watch this Scientific Journal Video about Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research at JoVE.com

作者想感谢她的帮助与生产该视频文章吉特沃纳博士。这项研究是由美国国家科学基金会职业奖。

第1部分：实验准备已批准的机构动物护理和使用委员会在波士顿大学鲎进行的实验程序。动物从海洋生物实验室（伍兹霍尔，MA）或其他供应商购买和住在曝气盐水池，接触到一个稳定的明暗周期的一个房间。引气蟹的生物钟，每天骑自行车之间的白天和夜间的状态的眼睛是非常重要的照明方案。任何侵入性的程序开始之前，立即冷冻动物是在10-15分钟的冰桶中，直至其运动速...

<ol>
	<li>Barlow, R. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Circadian+rhythms+in+the+Limulus+visual+system.">Circadian rhythms in the Limulus visual system.</a> J. Neurosci. 3, 856-870 (1983).</li><li>Passaglia, C. L., Dodge, F. A., Barlow, R. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+based+model+of+the+Limulus+lateral+eye.">Cell based model of the Limulus lateral eye.</a> J. Neurophysiol. 80, 1800-1815 (1998).</li><li>Snodderly, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Processing+of+visual+inputs+by+the+brain+of+Limulus.">Processing of visual inputs by the brain of Limulus.</a> J. Neurophysiol. 34, 588-611 (1971).</li><li>Passaglia, C., Dodge, F., Herzog, E., Jackson, S., Barlow, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Deciphering+a+neural+code+for+vision.">Deciphering a neural code for vision.</a> Proc. Natl. Acad. Sci. 94, 12649-12654 (1997).</li><li>Barlow, R. B., Kaplan, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Limulus+lateral+eye:+properties+of+receptor+units+in+the+unexcised+eye.">Limulus lateral eye: properties of receptor units in the unexcised eye.</a> Science. 174, 1027-1029 (1971).</li><li>Bolbecker, A. R., Lewis, A. R., Swan, A. A., Carlson, K., Fleet, J. R., Beck, K. E., Wasserman, G. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stable+Bellows+Cup+Electrode+Demonstrates+Low-frequency+Properties+of+Long-term+Electroretinographic+Recordings+in+the+Limulus+Lateral+Eye.">Stable Bellows Cup Electrode Demonstrates Low-frequency Properties of Long-term Electroretinographic Recordings in the Limulus Lateral Eye.</a> J. Neurosci. Meth. 159, 252-260 (2007).</li></ol>

我们已经演示了如何执行ERG的录音，视神经录音，并在体内的马蹄蟹的视网膜内的录音。每个录音技术都提供了视觉神经基础不同的见解，他们都可以被用来研究在视网膜功能的活的动物感谢螃蟹的大眼睛和坚硬甲壳。视神经活动，甚至可以自由表现在海洋动物电极（4）正确的施工记录。这些技术也可以进行切除眼睛轻微修改设置。自然条件等实验的相关性有限，为的蟹的眼睛变化的生理属性时...

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		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>LED</td>
<td>Light source</td>
<td>Newark Inc</td>
<td>33C1292</td>
<td>&nbsp;</td>
<tr>
<td>Suction electrode</td>
<td>Electrode</td>
<td>A-M Systems, Inc</td>
<td>573000</td>
<td>&nbsp;</td>
<tr>
<td>XCell 3*4-Channel Extracellular Amplifier</td>
<td>Amplifier</td>
<td>FHC Inc.</td>
<td>40-40-8B</td>
<td>&nbsp;</td>
<tr>
<td>Intracellular Recording</td>
<td>Amplifier</td>
<td>Cygnus</td>
<td>IR-283A</td>
<td>&nbsp;</td>
<tr>
<td>APM</td>
<td>Neural Spike Discriminator</td>
<td>FHC Inc.</td>
<td>APM</td>
<td>&nbsp;</td>
<tr>
<td>Bits++</td>
<td>Video Board</td>
<td>Cambridge Research Systems Inc</td>
<td>Bits++</td>
<td>&nbsp;</td>
<tr>
<td>Piezopatch Manipulator</td>
<td>Micropositioner</td>
<td>WPI Inc</td>
<td>PPM5000</td>
<td>&nbsp;</td>
<tr>
<td>Square Pulse Stimulator</td>
<td>Nerve Stimulator</td>
<td>Grass Instrument Division, Astro-Med, Inc.</td>
<td>Model S48</td>
<td>&nbsp;</td>
<tr>
<td>P-97</td>
<td>Micropipette Puller</td>
<td>Sutter Instruments</td>
<td>Model P-97</td>
<td>&nbsp;</td>
<tr>
<td>Borosilicate Glass Capillary</td>
<td>Electrode glass</td>
<td>WPI Inc</td>
<td>1B150-4</td>
<td>&nbsp;</td>
<tr>
<td>Horseshoe crab (Limulus polyphemus)</td>
<td>Animal</td>
<td>Marine Biological Laboratories</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Micropipette Puller</td>
<td>Glass Puller</td>
<td>Sutter Instruments</td>
<td>P-97</td>
<td>&nbsp;</td>
<tr>
<td>Zoom Stereoscope</td>
<td>Microscope</td>
<td>Jed Pella Inc.</td>
<td>SMZ-168</td>
<td>&nbsp;</td>		</tbody>
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using the horseshoe crab, limulus polyphemus, in vision research

美国鲎， 鲎波吕斐摩斯是地球上最古老的动物之一，与动物继续在生物医学研究中发挥了不可或缺的作用。不仅他们的血液中含有特殊的细胞，科学家们使用我们的药物检测bacteriotoxins，但他们的眼睛还包含一个神经网络，提供了有关在我们的视觉系统的生理过程，如光适应和侧抑制，很大的启示。鲎仍然是一个视觉研究的吸引力模型，因为动物是大型和强壮的无脊椎动物，其视网膜神经细胞又大又方便，其视觉系统是紧凑和广泛的研究，其视觉行为是明确界定。此外，眼睛的结构和功能上由一个动物的大脑中的生物钟，每天调制。总之，马蹄蟹的视觉系统是很简单的理解还够复杂，很有趣。 在这个视频中，我们目前调查的基础上，可在体内进行与鲎视觉神经的电生理模式。他们是电图记录，录音视神经和视网膜内的录音。电图（ERG）记录与衡量一个表面电极的电反应在一道闪光的眼睛的所有细胞中总结。它们可以被用来监测时间的延长期的整体灵敏度的眼睛。视神经录音衡量单一的神经纤维与外microsuction电极的扣球活动。它们可以被用来研究从眼睛传达到大脑以及昼夜时钟消息反馈从大脑到眼睛的视觉信息。视网膜内的录音与细胞内微电极测量电压波动引起的光在眼睛的单个细胞。它们可以被用来阐明视网膜处理的细胞机制。

Watch this Scientific Journal Video about Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research at JoVE.com

Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research

在这个视频中，我们执行与美国鲎电图记录，录音视神经和视网膜内的录音，<em&gt;鲎波吕斐摩斯</em&gt;。这些电的模式，可用于调查的视野，在研究或教学实验室的神经基础。

马蹄蟹，鲎波吕斐摩斯，在视觉研究

The American horseshoe crab, Limulus Polyphemus is one of the oldest creatures on earth, and the animal continues to play an indispensable role in ...

Research

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Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research

Blood Collection from the American Horseshoe Crab, Limulus Polyphemus

从美国的马蹄蟹，鲎波吕斐摩斯采血车

The horseshoe crab has the best-characterized immune system of any long-lived invertebrate.  The study of immunity in horseshoe crabs has been facilitated ...

科研

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This article describes the methods of measuring the toxicity of ultraviolet (UV) radiation and ocular toxins on primary (pHCEC) and immortalized (iHCEC) ...

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硝基还原酶/甲硝唑介导的消融和MATLAB平台（RpEGEN）研究斑马鱼视网膜色素上皮的再生

The retinal pigment epithelium (RPE) resides at the back of the eye and performs functions essential for maintaining the health and integrity of adjacent ...

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Transition metals such as Zn2+ ions must be tightly regulated due to their cellular toxicity. Previously, the activity of Zn2+ transporters was measured ...

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TEM Workflows, Results Analysis, and Data Management Using a Machine-Vision Approach

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