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DOI :
10.3791/1398-v
•
20:23 min
October 1st, 2009
Chapters
0:00
Title
0:55
Introduction
1:42
Preparing Lectin-Conjugated POROS Columns
5:15
Packing the Lectin-POROS Beads into a PEEK Column
8:11
HPLC of Samples
11:49
Desalting the Collected Fractions and PNGase F-digestion
16:34
Glycopeptide Analysis Representative Results
19:45
Conclusion
14:50
Ziptip Desalting of PNGaseF Samples
凝集素结合POROS珠被雇用的高效液相色谱法。糖肽类标准担任阳性和阴性对照。 MARS的14枯竭,胰蛋白酶消化人体血浆层析和流过(FT)和约束分数ESI-LC-MS/MS分析收集。糖肽的富集在绑定的分数相比,FT。
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