Name: the microfluidic probe: operation and use for localized surface processing
Uploaded: 2009-06-04T00:00:00
Description: Watch this Scientific Journal Video about The Microfluidic Probe: Operation and Use for Localized Surface Processing at JoVE.com

<p class="jove_content">这项工作是由全宗DE RECHERCHE SUR LA自然ET LES技术魁北克，加拿大创新研究院和加拿大卫生研究所（CIHR）fundation。 </p>

<p class="jove_title"> 1。微细探针头（不显示在视频的过程） </p><ol><li>一个<sup>2</sup>硅晶片，4英寸直径，厚525微米，具有1微米厚的热的SiO2层，spincoated与光阻（PR）为在4000转45的小号。 </li><li>晶圆是在110C预焙为50秒，并通过一个5秒的所有元素（端口和微），直接投资开发和冲洗的掩码公开。 </li><li>发现二氧化硅是铭刻在一个1:7缓冲氢氟酸（BHF）≈15分钟（已铭刻二氧化硅基板去湿指示完成蚀刻）的解决方案。一个氧?...

<ol>
	<li>Juncker, D., Schmid, H., Delamarche, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Nature Materials</em>. <b>4</b> (8), 622-622 (2005).</li></ol>

<p class="jove_content">微流体探头（MFP）是多才多艺的，因为它是（i）流动，（二）使用的试剂和底物的不同类型的适应性，它可以（三）大面积经营。 </p><p class="jove_content">有害泡沫可能导致的流量，以防止气泡的破坏，所有的组件需要充满液体大会前。探头和表面之间的差距只有几微米，但梅萨是几百微米，宽，并在厘米范围内的扫描距离。因此，这两个横向扫描面和MFP的台面与基板之间的并行需要非常谨慎地调?...

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		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>microfluidic connectors</td>
<td>&nbsp;</td>
<td>Upchurch Scientific</td>
<td>Micro- and Nano-tight fittings and sleeves</td>
<td>&nbsp;</td>
<tr>
<td>2-component manual dispenser</td>
<td>&nbsp;</td>
<td>Conprotec Inc.</td>
<td>DM400</td>
<td>To dispense and mix PDMS mixture</td>
</tr>
<tr>
<td>LabVIEW</td>
<td>&nbsp;</td>
<td>National Instruments</td>
<td>Version 8.0</td>
<td>&nbsp;</td>
<tr>
<td>Mechanical Convection Oven</td>
<td>&nbsp;</td>
<td>VWR</td>
<td>1330FM</td>
<td>&nbsp;</td>
<tr>
<td>Glass syringes</td>
<td>&nbsp;</td>
<td>Hamilton</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Capillary tubing</td>
<td>&nbsp;</td>
<td>Polymicro Technologies</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Plasma Chamber</td>
<td>&nbsp;</td>
<td>Tegal Corporation</td>
<td>Plasmaline 415</td>
<td>&nbsp;</td>
<tr>
<td>Inverted Microscope</td>
<td>&nbsp;</td>
<td>Nikon</td>
<td>TE2000-E</td>
<td>&nbsp;</td>
<tr>
<td>Syringe pumps</td>
<td>&nbsp;</td>
<td>Cetoni</td>
<td>neMESYS</td>
<td>&nbsp;</td>
<tr>
<td>Sylgard 184</td>
<td>&nbsp;</td>
<td>Ellsworth Adhesives</td>
<td>184 Sil Elast Kit</td>
<td>&nbsp;</td>
<tr>
<td>Camera</td>
<td>&nbsp;</td>
<td>Photometrics</td>
<td>QuantEM 512SC</td>
<td>&nbsp;</td>
<tr>
<td>Microscope stage</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Microfluidic probe holder goniometers</td>
<td>&nbsp;</td>
<td>Melles-Griot</td>
<td>07GON504</td>
<td>&nbsp;</td>
<tr>
<td>Linear stage</td>
<td>&nbsp;</td>
<td>Applied Scientific Instrumentation</td>
<td>LS-50</td>
<td>For z-control of the MFP</td>
</tr>
<tr>
<td>Manual linear stage</td>
<td>&nbsp;</td>
<td>Newport</td>
<td>443-4 Series</td>
<td>For x- and y- axis control of the MFP</td>
</tr>
<tr>
<td>Microscope stage</td>
<td>&nbsp;</td>
<td>Applied Scientific Instrumentation</td>
<td>PZ-2000</td>
<td>With x-, y- and z- control</td>
</tr>		</tbody>
		</table>
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the microfluidic probe: operation and use for localized surface processing

<p class="jove_content">微流体装置允许使用微量的样品进行检测，最近被用来控制细胞的微环境。通常与微流体样品可以引进，并在培养细胞的情况下，在一个密闭的卷，封闭的，限制其使用的微。另一方面，micropipetting系统已用于本地灌注细胞和表面的，尤其是使用推挽设置，其中源和其他如水槽，但流动的禁闭吸管行为很难在三个方面。此外，吸液管是脆弱的，难以定位，因此，只使用静态配置。 </p><p class="jove_content">微流体探头（MFP）避开封闭的微流体通道的建设和实施的限制，微流体流动，而不是封闭到微流体系统样品，可以直接传送到样品，并扫描整个样品，使用MFP。 。注射和愿望开口都设在一个几十微米，使注入的差距只限于一个微水刀周围的液体的水动力和完全吸气到其他开放。微水刀可以在基板表面被刷新，并提供了本地化的试剂可用于大面积扫描探针在表面上的沉积/交付精确的工具。在这个视频中，我们目前的微流<sup>控</sup>探头1（MFP ）。我们详细解释如何组装MFP，倒置显微镜上面安装，并对齐相对基板表面，并最终显示如何使用它的过程中衬底表面，沉浸在一个缓冲区。 </p>

Watch this Scientific Journal Video about The Microfluidic Probe: Operation and Use for Localized Surface Processing at JoVE.com

The Microfluidic Probe: Operation and Use for Localized Surface Processing

<p class="jove_content">在这个视频中，我们目前微流体探头<sup&gt; 1</sup（MFP）。我们详细解释如何组装MFP，倒置显微镜上面安装，并对齐相对基板表面，并最终显示了如何使用它在缓冲溶液中浸泡处理基材表面。</p>

微探针：操作和使用的局部表面处理

Microfluidic devices allow assays to be performed using minute amounts of sample and have recently been used to control the microenvironment of cells. ...

Research

JoVE Journal

Biology

Localized RNAi and Ectopic Gene Expression in the Medicinal Leech

本地化的RNAi和药用水蛭异位基因表达

In this video, we show the use of a pneumatic capillary gun for the accurate biolistic delivery of reagents into live tissue. We use the procedure to ...

科研

生物学

Digital Microfluidics for Automated Proteomic Processing

数字微流体进行自动蛋白质组学处理

Clinical proteomics has emerged as an important new discipline, promising the discovery of biomarkers that will be useful for early diagnosis and ...

Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures 

为贴壁细胞培养的氧气插入的制备及操作

Oxygen is a key modulator of many cellular pathways, but current devices permitting in vitro oxygen modulation fail to meet the needs of biomedical ...

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

生物物理检测探头相间细胞核的力学性能：基材应变的应用和微针操纵

In most eukaryotic cells, the nucleus is the largest organelle and is typically 2 to 10 times stiffer than the surrounding cytoskeleton; consequently, the ...

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

Avidity-based Extracellular Interaction Screening AVEXIS for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

基于亲和力，外的相互作用筛选（AVEXIS）外低亲和力受体 - 配体相互作用的可扩展检测

Extracellular protein:protein interactions between secreted or membrane-tethered proteins are critical for both initiating intercellular communication and ...

The Use of Chemostats in Microbial Systems Biology

恒化器中微生物系统生物学中的应用

Cells regulate their rate of growth in response to signals from the external world. As the cell grows, diverse cellular processes must be coordinated ...

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

导管内注射局部给药的小鼠乳腺

Herein we describe a protocol to deliver various reagents to the mouse mammary gland via intraductal injections. Localized drug delivery and knock-down of ...

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

膜蛋白的实时测量：受体相互作用使用表面等离子体共振（SPR）

Protein-protein interactions are pivotal to most, if not all, physiological processes, and  understanding the nature of such interactions is a central ...

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

基于探针实时荧光定量PCR途径的小分子RNA定量检测

Probe-based quantitative PCR (qPCR) is a favoured method for measuring transcript abundance, since it is one of the most sensitive detection methods that ...

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

干血斑点. 免疫和分子技术中使用的制备和加工

The idea of collecting blood on a paper card and subsequently using the dried blood spots (DBS) for diagnostic purposes originated a century ago. Since ...