Name: visualizing the beating heart in drosophila
Uploaded: 2009-09-28T21:00:00
Description: Watch this Scientific Journal Video about Visualizing the Beating Heart in Drosophila at JoVE.com

Ko是支持由美国心脏协会的资助。

在您开始之前成人心<ol><li>刚准备人工血淋巴（AH）的解决方案包含108MM娜5mm的K +，2mm的钙2 +，8MM氯化镁2，1MM的NaH 2 PO 4，4MM碳酸氢钠3，10mm的蔗糖，5mm的海藻糖，5MM的HEPES（pH值7.1，所有的试剂从西格玛化学品）。蔗糖和海藻糖应添加到AH冷藏库存解决方案在使用前，为了防止细菌污染。 </li><li>带来AH室温至少15分钟的含氧空气鼓泡解决?...

<ol>
	<li>Ocorr, K., Reeves, N., Wessells, R. J., Fink, M., Chen, H. -. S. V., Akasaka, T., Yasuda, S., Metzger, J., Giles, W., Posakony, J. W., Bodmer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=KCNQ+potassium+channel+mutations+cause+cardiac+arrhythmias+in+Drosophila+that+mimic+the+effects+of+aging.">KCNQ potassium channel mutations cause cardiac arrhythmias in Drosophila that mimic the effects of aging.</a> Proc Natl Acad Sci U S A. 104, 3943-3948 (2007).</li><li>Broadie, K. S., Bate, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+the+embryonic+neuromuscular+synapse+of+Drosophila+melanogaster.">Development of the embryonic neuromuscular synapse of Drosophila melanogaster.</a> J Neurosci. 13, 144-166 (1993).</li></ol>

果蝇模型已被证明是一个强大的遗传工具，已经被用来解决各种科学问题，从胚胎发育，学习和记忆不等。最近这种多功能的模式生物用于研究在果蝇心脏功能的基因。一些试图量化在成年果蝇的心脏生理依赖于完整的苍蝇经腹角质层的意见。这些方法大多依靠肉眼观察或改变录音，通过腹部的量化一个参数，心率在光照强度变送器。这些方法有一些限制：通常只有心前结束，可以观察到，我们?...

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<td>Micro Dissecting Spring Scissors (curved)</td>
<td>&nbsp;</td>
<td>Roboz</td>
<td>RS-5611</td>
<td>Good for gross cuts (Step 3)</td>
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<tr>
<td>Micro Dissecting Spring Scissors (straight)</td>
<td>&nbsp;</td>
<td>Roboz</td>
<td>RS-5620</td>
<td>&nbsp;</td>
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<td>Dumont #55 forceps</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>11295-51</td>
<td>&nbsp;</td>
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<td>Dumont #5 forceps</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
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<td>Glass Capillaries, 100ul</td>
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<td>Glass Capillaries, fine</td>
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<td>Pipette Puller</td>
<td>&nbsp;</td>
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<td>Both horizontal and vertical pipette pullers will work</td>
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<td>Plastic tubing</td>
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<td>Tygon R-3603</td>
<td>1/16&#x201D; inner diameter</td>
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<td>Plastic tubing</td>
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<td>Histoacryl&reg; tissue adhesive</td>
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visualizing the beating heart in drosophila

“<em&gt;果蝇</em&gt;心脏最近出现了一个很好的模型系统研究生肌心中的基本功能的基因，细胞和分子机制。在飞行检查心脏功能的一个关键的一步是寻找一种方法来访问的方式，保留其生肌的功能，同时仍然允许跳动的心脏器官进行观察和记录的心。两种不同的方法，观察和记录中的幼虫和成人心脏跳动<em&gt;果蝇</em&gt;这里描述。成蝇半完整的准备允许在不干扰色素的角质层和覆脂肪体腹部心脏清晰的可视化。记录幼虫心脏跳动，它是必要的，以固定的幼虫，最大限度地减少体壁运动，从而减少心脏运动不与心肌收缩。我们的方法产生稳定的成年和幼虫的心准备，可以在1-3赫兹率击败小时。

Watch this Scientific Journal Video about Visualizing the Beating Heart in Drosophila at JoVE.com

Visualizing the Beating Heart in Drosophila

技术所需的可视化的幼虫和成虫在跳动的心脏<em&gt;果蝇</em&gt;提交。每个人生阶段需要不同的方法。

可视化心脏跳动果蝇</em

The Drosophila heart has recently emerged as a good model system for examining the genetic, cellular, and molecular mechanisms underlying function in ...

Research

JoVE Journal

Biology

Visualizing the Beating Heart in Drosophila

Visualizing the Live Drosophila Glial-neuromuscular Junction with Fluorescent Dyes

Visualizing the Live Drosophila Glial-neuromuscular Junction with Fluorescent Dyes

可视化的Live果蝇胶质细胞神经​​肌肉交界处，与荧光染料

Our project identified GFP labeled glial structures at the developing larval fly neuromuscular synapse. To look at development of live glial-nerve-muscle ...

科研

生物学

Fluorescent Labeling of Drosophila Heart Structures

Fluorescent Labeling of Drosophila Heart Structures

荧光标记的果蝇心结构

The Drosophila melanogaster dorsal vessel, or heart, is a tubular structure comprised of a single layer of contractile cardiomyocytes, pericardial cells ...

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

在幼虫的监测心脏功能果蝇生理研究

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained  and  ...

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

电子显微镜对胚胎的制备果蝇胚胎心管

The morphogenesis of the Drosophila  embryonic heart tube has emerged as a valuable model system for studying cell migration, cell-cell adhesion and cell ...

Quantification of &gamma;H2AX Foci in Response to Ionising Radiation

Quantification of ÃƒÅ½Ã‚Â³H2AX Foci in Response to Ionising Radiation

电离辐射的反应定量的γH2AX灶

DNA double-strand breaks (DSBs), which are induced by either endogenous metabolic processes or by exogenous sources, are one of the most critical DNA ...

Quantitation of &gamma;H2AX Foci in Tissue Samples

Quantitation of ÃƒÅ½Ã‚Â³H2AX Foci in Tissue Samples

定量组织样本中的γH2AX灶

DNA double-strand breaks (DSBs) are particularly lethal and genotoxic lesions, that can arise either by endogenous (physiological or pathological) ...

Evaluation of the Spatial Distribution of &gamma;H2AX following Ionizing Radiation

Evaluation of the Spatial Distribution of ÃƒÅ½Ã‚Â³H2AX following Ionizing Radiation

评价以下电离辐射的空间分布的γH2AX

An early molecular response to DNA double-strand breaks (DSBs) is phosphorylation of the Ser-139 residue within the terminal SQEY motif of the histone ...

Clonogenic Assay: Adherent Cells

克隆形成含量：贴壁细胞

The clonogenic (or colony forming) assay has been established for more than 50 years; the original paper describing the technique was published in 19561. ...

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

凋亡细胞清除过程中实时成像<em&gt;果蝇</em&gt;胚胎发育

The  proper elimination of unwanted or aberrant cells through apoptosis and  subsequent phagocytosis (apoptotic cell clearance) is crucial for normal  ...

Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers

钙火花的评估在正常肌肉纤维

Maintaining homeostatic Ca2+ signaling is a fundamental physiological process in living cells. Ca2+ sparks are the elementary units of Ca2+ signaling in ...