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DOI :
10.3791/1433-v
September 17th, 2009
Chapters
0:00
Title
0:37
Introduction
1:18
Isolation of Adult Cardiac Myocytes from Rat Heart by Langendorrf Preparation
9:45
Confocal Imaging of 3D Subcellular Structures
8:02
Primary Cell Culture and Adenoviral Transduction
11:09
Imaging of Adult Rat Cardiac Myocytes Representative Results
11:43
Conclusion
成人心筋細胞は動物の心臓から分離し、数日間培養することができる主要な細胞である。この培養期間内にアデノウイルス遺伝子導入は、遺伝的に符号化されたバイオセンサー(GEBs)または蛍光融合タンパク質を発現させることができる。両方のアプローチは、共焦点顕微鏡による細胞の調査を許可する。
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