Name: semi-automated optical heartbeat analysis of small hearts
Uploaded: 2009-09-16T13:00:00
Description: Watch this Scientific Journal Video about Semi-automated Optical Heartbeat Analysis of Small Hearts at JoVE.com

<p class="jove_content"> KO和AC支持赠款和美国心脏协会的奖学金。 SIB和RB是支持由美国国立卫生研究院的资助。 </p>

<p class="jove_content"> <a href="https://www.jove.com/files/ftp_upload/1435/SOHA_overview.pdf">点击这里查看半自动化的心跳分析（所下）程序的概述</a> 。 </p><p class="jove_content">半自动光学心跳分析</p><p class="jove_title"> 1。预处理 - </p><p class="jove_content">这一步提供舒张压和收缩压的心直径的信息。 </p><p class="jove_content">心脏边缘识别​​和标记在最大的舒张和收缩。光学录音，可先进的速度缓慢，甚至在时间允许的最大收缩和放松的心?...

<ol>
	<li>Cammarato, A., Dambacher, C. M., Reedy, M. C., Knowles, A. F., Kronert, W. A., Bodmer, R., Ocorr, K., Bernstein, S. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myosin+Transducer+mutations+differentially+affect+motor+function,+myofibril+structure,+and+the+performance+of+skeletal+and+cardiac+muscles.">Myosin Transducer mutations differentially affect motor function, myofibril structure, and the performance of skeletal and cardiac muscles.</a> <em style='font-style: italic'>Mol Biol Cell</em>. <b>19</b> (2), 553-562 (2008).</li><li>Ocorr, K., Reeves, N., Wessells, R. J., Fink, M., Chen, H. -. S. V., Akasaka, T., Yasuda, S., Metzger, J., Giles, W., Posakony, J. W., Bodmer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=KCNQ+potassium+channel+mutations+cause+cardiac+arrhythmias+in+Drosophila+that+mimic+the+effects+of+aging.">KCNQ potassium channel mutations cause cardiac arrhythmias in Drosophila that mimic the effects of aging.</a> <em style='font-style: italic'>Proc Natl Acad Sci U S A</em>. <b>104</b>, 3943-3948 (2007).</li><li>Fink, M., Callol-Massot, C., Chu, A., Ruiz-Lozano, P., Izpisua Belmonte, J. C., Giles, W., Bodmer, R., Ocorr, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+method+for+the+detection+and+quantification+of+heartbeat+parameters+in+Drosophila,+zebrafish+and+embryonic+mouse+hearts.">A new method for the detection and quantification of heartbeat parameters in Drosophila, zebrafish and embryonic mouse hearts.</a> <em style='font-style: italic'>Biotechniques</em>. <b>46</b>, 101-113 (2009).</li></ol>

<p class="jove_content">果蝇模型已被证明是一个强大的遗传工具，已经被用来解决各种科学问题，从胚胎发育，学习和记忆不等。最近这种多功能的模式生物用于研究心脏功能的基因。一些试图量化在成年果蝇的心脏生理依赖于完整的苍蝇经腹角质层的意见。这些方法大多依靠目视观察，或通过腹部量化一个参数，心率传输光的强度变化的录音。虽然这是一个有用的参数，它是有限的，它告诉我们对心脏功能。我们的?...

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<td>MatLab software</td>
<td>&nbsp;</td>
<td>Math Works, Inc</td>
<td>&nbsp;</td>
<td>Required environment for the analysis software.</td>
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<td>EM-CCD digital camera</td>
<td>&nbsp;</td>
<td>Hamamatsu Corp.</td>
<td>9100 or 9300</td>
<td>Other high speed digital cameras will also work.</td>
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<td>HC image  data capture software</td>
<td>&nbsp;</td>
<td>Hamamatsu Corp.</td>
<td>&nbsp;</td>
<td>Other image capture software that produces movies in avi format will also work.</td>
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<td>Light Microscope with 10x objective </td>
<td>&nbsp;</td>
<td>Leica</td>
<td>&nbsp;</td>
<td>A dipping lens that eliminates the air water interface greatly improves resolution</td>
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semi-automated optical heartbeat analysis of small hearts

<p class="jove_content">我们已经开发出一种用于分析高速光学录音方法<em&gt;果蝇</em&gt;，斑马鱼和小鼠胚胎心（芬克等，2009）。我们的半自动光学心跳分析（所下）使用一种新的运动检测算法，该算法能够检测与个别的收缩和放松活动相关的心脏运动。该方案提供了有关生理的读数，包括收缩压和舒张压的时间间隔，心率，以及定性和定量的心跳arrhythmicity主机。该方案还计算出在心脏舒张和收缩缩短分数和小数的面积变化计算的直径测量。输出为数字文件，与大多数电子表格程序兼容。每一次心跳的测量是在提高统计输出功率纪录。我们证明每个用户输入所需的步骤，并显示我们的方法的应用在所有这三个基因听话的心脏模型的心功能分析。</p>

Watch this Scientific Journal Video about Semi-automated Optical Heartbeat Analysis of Small Hearts at JoVE.com

Semi-automated Optical Heartbeat Analysis of Small Hearts

<p class="jove_content">我们已经开发了一个半自动化光学检测信号分析方法（所下分析高速光学录音）<em&gt;果蝇</em&gt;，斑马鱼和小鼠胚胎心中。我们证明我们的方法在果蝇和小鼠胚胎心脏的心脏功能分析中的应用。</p>

半自动小红桃光学心跳分析

We have developed a method for analyzing high speed optical recordings from Drosophila, zebrafish and embryonic mouse hearts (Fink, et. al., 2009). Our ...

Research

JoVE Journal

Biology

Proper Care and Cleaning of the Microscope

适当的照顾和清洁的显微镜

Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a ...

科研

生物学

Major Components of the Light Microscope

光镜下的主要组成部分

The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for ...

Phase Contrast and Differential Interference Contrast (DIC) Microscopy

Phase Contrast and Differential Interference Contrast DIC Microscopy

相衬，微分干涉对比（DIC）显微镜

Phase-contrast microscopy is often used to produce contrast for transparent, non light-absorbing, biological specimens. The technique was discovered by ...

Optical Mapping of Langendorff-perfused Rat Hearts

光学测绘的Langendorff灌注大鼠心脏

Optical mapping of the cardiac surface with voltage-sensitive fluorescent dyes has become an important tool to investigate electrical excitation in ...

Substrate Generation for Endonucleases of CRISPR/Cas Systems

CRISPR / CAS系统内切酶的底物生成

The  interaction of viruses and their prokaryotic hosts shaped the evolution of  bacterial and archaeal life. Prokaryotes developed several strategies to ...

LeafJ: An ImageJ Plugin for Semi-automated Leaf Shape Measurement

LeafJ：ImageJ的插件半自动叶形状测量

High throughput phenotyping (phenomics) is a powerful tool for linking genes to their functions (see review1 and recent examples2-4). Leaves are the ...

Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos

组织特异性荧光的斑马鱼胚胎半自动成像

Zebrafish embryos are a powerful tool for large-scale screening of small molecules. Transgenic zebrafish that express fluorescent reporter proteins are ...

Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis

从固定心脏分离心肌细胞进行免疫细胞化学和倍体分析

The adult mammalian heart is composed of various cell types including cardiomyocytes, endothelial cells and fibroblasts. Since it is difficult to reliably ...

Small-Scale Plasma Membrane Preparation for the Analysis of <em>Candida albicans</em> Cdr1-mGFPHis

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

用于分析 <em>念珠菌</em> Cdr1-mGFPHis分析的小尺度等离子膜制备

The successful biochemical and biophysical characterization of ABC transporters depends heavily on the choice of the heterologous expression system. Over ...

Quantitative Analysis of Viscoelastic Properties of Red Blood Cells Using Optical Tweezers and Defocusing Microscopy

使用光学镊子和散焦显微镜定量分析红细胞的粘弹性

The viscoelastic properties of erythrocytes have been investigated by a range of techniques. However, the reported experimental data vary. This is not ...