Gli autori ringraziano Christiane Pahrmann (Lab Manager).

<ol><li> Maschio topi BALB / c (8-12 settimane) sono acquistati da Charles River Laboratories (Sulzfeld, Germania). I topi sono alloggiati in condizioni convenzionali, i topi nutriti standard di cibo e acqua ad libitum. </li><li> 2% Isofluran viene utilizzato per l&#39;anestesia. </li></ol> DONATORE DI PREPARAZIONE <ol><li> Radersi i capelli addominale e disinfettare la zona con betaisodona. </li><li> In vista microscopico, eseguire una incisione mediana cervicale dal livello della laringe allo sterno. </li><li> Rimuov...

<ol>
	<li>Adams, B., Berry, G., Huang, X., Shorthouse, R., Brazelton, T., Morris, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Immunosuppressive+therapies+for+the+prevention+and+treatment+of+obliterative+airway+disease+in+heterotopic+rat+trachea+allografts.">Immunosuppressive therapies for the prevention and treatment of obliterative airway disease in heterotopic rat trachea allografts.</a> Transplantation. 69, 2260-2266 .</li><li>Adams, B., Brazelton, T., Berry, G., Morris, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+respiratory+epithelium+in+a+rat+model+of+obliterative+airway+disease.">The role of respiratory epithelium in a rat model of obliterative airway disease.</a> Transplantation. 69, 661-665 .</li><li>Deuse, T., Schrepfer, S., Reichenspurner, H., Hoyt, G., Fischbein, M., Robbins, R., Pelletier, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Techniques+for+experimental+heterotopic+and+orthotopic+tracheal+transplantations+&amp;#x2013;+when+to+use+which+model;+Transplant+Immunology+17.">Techniques for experimental heterotopic and orthotopic tracheal transplantations &amp;#x2013; when to use which model; Transplant Immunology 17.</a> , 255-261 (2007).</li><li>Hele, D., Yacoub, M., Belvisi, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+heterotopic+tracheal+allograft+as+an+animal+model+of+obliterative+bronchiolitis.">The heterotopic tracheal allograft as an animal model of obliterative bronchiolitis.</a> Respiratory Research. 2, 169-183 .</li><li>Hertz, M., Jessurun, J., King, M., Savic, S., Murray, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reproduction+of+the+obliterative+bronchiolitis+lesion+after+heterotopic+transplantation+of+mouse+airways.">Reproduction of the obliterative bronchiolitis lesion after heterotopic transplantation of mouse airways.</a> American Journal of Pathology. 142, (1993).</li><li>McDyer, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+and+murine+obliterative+bronchiolitis+in+transplant.">Human and murine obliterative bronchiolitis in transplant.</a> Proceedings of the American Thoracic Society. 4, 37-43 .</li><li>Schrepfer, S., Deuse, T., Hoyt, G., Sheikh, A., Hoffmann, J., Reichenspurner, H., Robbins, R., Pelletier, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+orthotopic+tracheal+transplantation:+the+stanford+technique.">Experimental orthotopic tracheal transplantation: the stanford technique.</a> Microsurgery. 27, 187-189 (2007).</li></ol>

I topi sono disponibili in diverse transgeneic e modello ad eliminazione diretta, e quindi adatto per lo studio meccanicistico domande relative a OAD (4). Entrambi i modelli di trapianto della trachea mostrato in questo video può essere utilizzato come modello affidabile per lo studio dello sviluppo OAD. Tuttavia, ogni modello dimostra vantaggi e limiti. Il trapianto eterotopico tracheale è facile da eseguire e non richiede particolari formazione chirurgica (...

heterotopic and orthotopic tracheal transplantation in mice used as models to study the development of  obliterative airway disease

Obliterante malattia delle vie aeree (OAD) è la complicazione maggiore dopo trapianto del polmone che limita la sopravvivenza a lungo termine (1-7). Per studiare la fisiopatologia, il trattamento e la prevenzione di OAD, diversi modelli animali di trapianto della trachea nei roditori sono state sviluppate (1-7). Qui, usiamo due modelli consolidati di trapianto di trachea, il modello eterotopico e ortotopica e dimostrare la loro vantaggi e limiti. Per il modello eterotopico, la trachea del donatore è avvolto nel più grande omento del destinatario, mentre la trachea del donatore viene anastomizzato da end-to-end anastomosi nel modello ortotopico. In entrambi i modelli, lo sviluppo di lesioni istologiche obliterante simile a OAD clinica è stato dimostrato (1-7). Questo video mostra come eseguire entrambi, il eterotopica così come la tecnica di trapianto ortotopico tracheale nei topi, e mette a confronto l&#39;andamento temporale di sviluppo OAD in entrambi i modelli con istologia.

Watch this Scientific Journal Video about Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of  Obliterative Airway Disease at JoVE.com

Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of  Obliterative Airway Disease

Questo video mostra e mette a confronto due modelli sperimentali per studiare lo sviluppo della malattia delle vie aeree obliterante (OAD) nei topi, il modello eterotopico e trapianto ortotopico tracheale.

Eterotopica e trapianto ortotopico tracheale in topi utilizzati come modelli per studiare lo sviluppo della malattia delle vie aeree obliterante

Obliterative airway disease (OAD) is the major complication after lung transplantations that limits long term survival (1-7).
To study the ...

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