Name: lad-ligation: a murine model of myocardial infarction
Uploaded: 2009-10-14T23:30:00
Description: Watch this Scientific Journal Video about LAD-Ligation: A Murine Model of Myocardial Infarction at JoVE.com

作者感谢她的技术援助和执行组织学克里斯蒂安Pahrmann。

<html>年龄在8至12周的最低重量为20g的BALB / C小鼠均购自查尔斯河（Sandhofer WEG 7，D - 97633 Sulzfeld）。 小鼠被安置在常规条件下，美联储的标准鼠标颗粒和水自由采食 。麻醉与异氟醚使用感应室（2％）的鼠标。 <ol><li>剃除颈部，左侧的肋骨和消毒用80％乙醇。 </li><li>在它的后面放置鼠标放在它的鼻子和嘴的口罩，以保持麻醉。 </li><li>检查的反射捏尾巴和后腿的脚，以确保足够anesthesized...

<ol>
	<li>Atkins, B. Z., Hueman, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cellular+cardiomyoplasty+improves+diastolic+properties+of+injured+Heart.">Cellular cardiomyoplasty improves diastolic properties of injured Heart.</a> J Surg Res. 85 (2), 234-242 (1999).</li><li>Jaquet, K., KT, K. r. a. u. s. e. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduction+of+myocardial+scar+size+after+implantation+of+mesenchymal+stem+cells+in+rats:+what+is+the+mechanism.">Reduction of myocardial scar size after implantation of mesenchymal stem cells in rats: what is the mechanism.</a> Stem Cells Dev. 14 (3), 299-309 (2005).</li><li>Joung, B., Kim, I. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bone+marrow+mononuclear+stem+cells+transplanted+in+rat+infarct+myocardium+improved+the+electrical+conduction+without+evidence+of+proarrhythmic+effects.">Bone marrow mononuclear stem cells transplanted in rat infarct myocardium improved the electrical conduction without evidence of proarrhythmic effects.</a> Yonsei Med J. 48 (5), 754-764 (2007).</li></ol>

综上所述，本视频一步一步的LAD结扎模型可视化和标识在手术过程中很容易可行的小鼠法援署义务。

lad-ligation: a murine model of myocardial infarction

梗死和心肌缺血的研究模型是必不可少的调查在心肌缺血的急性和慢性的病理生物学和病理生理过程，并开发和优化未来的治疗。 两种不同的方法建立心肌缺血是在啮齿类实验动物。第一种方法是创建一个快速但不精确的技术，冷冻梗死，其中一个冷冻笔的心（1-3）表面上应用。科学家使用这种方法不能保证冷冻疤痕导致缺血，也显示病理生理方是不相关的心肌梗死的效果，创建了广阔的心肌损伤。第二种方法是永久性结扎左冠状动脉前降支（LAD）。这里法援署是一个单针结扎，形成了一个几乎立即可以看到的缺血。关闭法援署，没有进一步的血流量是允许在这一领域，而周围的心肌组织中几乎没有受到影响。这种外科手术模拟发生在梗死心肌缺血的病理生物学和病理生理方面。 在这个视频中介绍的方法演示鼠标梗死模型的外科手​​术结扎法援署。这种模式是心肌梗死的病理生物学和病理生理以及免疫生物学研究的方便。显示技术提供高精确度和关联以及与组织学切片。

Watch this Scientific Journal Video about LAD-Ligation: A Murine Model of Myocardial Infarction at JoVE.com

LAD-Ligation: A Murine Model of Myocardial Infarction

本视频演示了如何使用快捷及可靠的模型来研究心肌缺血的病理生物学和病理生理过程。

法援署结扎：有心肌梗死的小鼠模型

Research models of infarction and myocardial ischemia are essential to investigate the acute and chronic pathobiological and pathophysiological processes ...

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