Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/1447-v
•
11:48 min
November 13th, 2009
Chapters
0:00
Title
0:09
Introduction
0:30
Viral Transduction of Mouse Embryonic Fibroblasts
1:25
Doxycycline Induced Reprogramming
3:18
Immunocytochemical Analysis of Transduction Efficiency
5:31
Isolating and Expanding Induced Pluripotent Stem Cell Colonies
7:39
Immunocytochemical Analysis for Pluripotency
9:14
Pluripotency Marker Analysis
10:31
Conclusion
Stemgent阿霉素诱导小鼠TF慢病毒设置可以重新编程诱导多能干细胞(iPS细胞)的小鼠胚胎成纤维细胞(MEFs)。在这里,我们展示了鼠标重新编程转录因素Oct4的DOX诱导表达的协议,SOX2,KLF4和c - Myc基因产生的iPS殖民地,表达共同的MES多能性标志物。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved