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DOI :
10.3791/1470-v
August 7th, 2009
Chapters
0:05
Title
1:03
Extraction of Total RNA using TRIzol Reagent
0:41
Introduction
7:57
cDNA Synthesis using the Invitrogen SuperScript First-Strand System
3:55
RNA Cleanup using the Qiagen RNEasy Mini Kit
10:28
cDNA Isolation and Precipitation
14:57
Conclusion
13:50
Representative Results/Outcome
高品質の単離は、インタクトなRNAは、多くの実験室のプロトコルで必須の工程である。ここで、我々は全体のゼブラフィッシュの胚と遺伝子発現のマイクロアレイ解析を含む様々な実験手順で、後続のアプリケーションのためのcDNA合成からのRNA抽出を示しています。
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