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DOI :
10.3791/1499-v
October 2nd, 2009
Chapters
0:00
Title
0:46
Introduction
1:15
Transfection of 293 Cells To Produce Lentivirus
3:24
Lentivirus Harvest
4:39
Lentivirus Concentration via Ultracentrifugation
7:24
Lentivirus Titration Using Flow Cytometry
11:17
Conclusion
为了使慢病毒,DNA载体转染到人293细胞。收获后,集中上清液,荧光表达,流式细胞仪,是由病毒滴度。
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