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DOI :
10.3791/1519-v
•
15:02 min
September 22nd, 2009
Chapters
0:00
Title
0:54
Introduction
2:09
Microdissection of Explants
7:16
Microdissection and Culture of Central Explants
9:18
Harvesting the Explants for Protein Analysis
10:19
Harvesting the Explants for Immunofluorescence
12:15
Immunofluorescence and Protein Analysis Results
15:54
Conclusion
在FGF - 2的存在培养的大鼠晶状体上皮细胞的中部地区的外植体分化同步。这种文化免疫荧光显微镜,可以提供有关新的信息与终末分化相关的基因表达和信号事件。
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