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DOI :
10.3791/1519-v
•
15:02 min
September 22nd, 2009
Chapters
0:00
Title
0:54
Introduction
2:09
Microdissection of Explants
7:16
Microdissection and Culture of Central Explants
9:18
Harvesting the Explants for Protein Analysis
10:19
Harvesting the Explants for Immunofluorescence
12:15
Immunofluorescence and Protein Analysis Results
15:54
Conclusion
FGF - 2の存在下で培養するとラットレンズ上皮の中心領域の外植片は、同期的に分化する。そのような文化の免疫蛍光顕微鏡は、遺伝子発現と分化に関連付けられているシグナリングイベントに関する新たな情報を提供することができます。
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