Name: culture of mouse neural stem cell precursors
Uploaded: 2007-02-25T00:00:00
Description: Watch this Scientific Journal Video about Culture of Mouse Neural Stem Cell Precursors at JoVE.com

<ol><li>隔离E12.5胚胎皮层神经前体鼠标（筹备）。 </li><li>从解剖telencephalic水泡，皮肤和间质层被拆除。 </li><li>囊泡在0.05％的胰蛋白酶与0.02％EDTA和0.2％，在20分钟的HBSS牛血清白蛋白在37 ° C孵育</li><li>胰蛋白酶是由1 mg / ml的大豆胰蛋白酶抑制剂等体积的HBSS（Sigma公司＃T6522）停止。 </li><li>组织消化是分离的火抛光的巴斯德移液器使用几轮trituration。 </li><li>细胞被洗涤一次，0.2％在HBSS中牛血清白蛋白和5万细胞/层粘?...

<ol>
	<li>Currle, D., Cheng, X., Hsu, C., Monuki, E., Flanagan, L., Rebaza, L., Derzic, S., Schwartz, P., Monuki, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Direct+and+indirect+roles+of+CNS+dorsal+midline+cells+in+choroid+plexus+epithelial+formation.">Direct and indirect roles of CNS dorsal midline cells in choroid plexus epithelial formation.</a> <em style='font-style: italic'>Development</em>. <b>132</b> (15), 3549-3559 (2005).</li></ol>

<p>我们的收获，分离和培养胚胎神经干细胞的能力，在我们的中枢神经系统发育和干细胞生物学的理解的巨大进步已成为可能。本视频演示E12.5小鼠大脑皮层和随后的分类和胚胎神经干细胞培养的夹层。许多其他的其他类似的方法已成功地采用了其他的调查。 </p>

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culture of mouse neural stem cell precursors

<p class="jove_content">主神经干细胞培养为研究中枢神经系统发育的内在机制，有用的。干细胞研究将增加我们的神经系统的了解，可能使我们能够发展为目前无法治愈的大脑疾病和损伤的治疗。此外，应使用干细胞，干细胞的研究，在神经细胞分化和转分化的机制和遗传和环境信号的详细研究，直接成特定的细胞类型的细胞的专业化。本视频演示了从胚胎12.5天鼠标背侧前脑细胞用来分解的一种技术。剥离过程包括收获E12.5小鼠胚胎，从子宫精细解剖钳去除的“皮肤”，最后隔离件大脑皮层。解剖后，该组织是消化和机械分离。悬浮分离细胞，然后培养在“干细胞”的媒体，有利于神经干细胞的生长。</p>

Watch this Scientific Journal Video about Culture of Mouse Neural Stem Cell Precursors at JoVE.com

Culture of Mouse Neural Stem Cell Precursors

<p class="jove_content">本视频介绍了从胚胎小鼠的发展皮层neuroprecursors隔离所采用的方法。胚胎从子宫中取出，解剖皮层组织，消化孤立的大脑皮层的过程。</p>

培养小鼠神经干细胞的前体

Primary neural stem cell cultures are useful for studying the mechanisms underlying central nervous system development. Stem cell research will increase ...

Research

JoVE Journal

Biology

Dissection of 6.5 dpc Mouse Embryos

6.5 DPC小鼠胚胎剖析

Analysis of gene expression patterns during early stages of mammalian embryonic development can provide important clues about gene function, cell-cell ...

科研

生物学

Immunocytochemistry: Human Neural Stem Cells

免疫组化：人类神经干细胞

Immunocytochemistry is a very powerful and fairly straightforward method for determining the presence, subcellular localization, and relative abundance of ...

Culture of myeloid dendritic cells from bone marrow precursors

文化髓系树突状细胞从骨髓前体

Myeloid dendritic cells (DCs) are frequently used to study the interactions between innate and adaptive immune mechanisms and the early response to ...

Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

Mouse Epidermal Neural Crest Stem Cell EPI-NCSC Cultures

小鼠表皮神经嵴干细胞（EPI的非公务员合约雇员）文化

EPI-NCSC are remnants of the embryonic neural crest in an adult location, the bulge of hair follicles. They are multipotent stem cells that have the ...

Generation of Single-Cell Suspensions from Mouse Neural Tissue

从小鼠神经组织产生单细胞悬浮液

Within the nervous system, hundreds of neuronal and glial cell types have been described. Each specific cell type in the brain or spinal cord has a ...

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

胚胎干细胞分化成少突胶质前体

Oligodendrocytes are the myelinating cells of the central nervous system. For regenerative cell therapy in demyelinating diseases, there is significant ...

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

高效的推导人类心脏的前体和从多能干的小分子诱导人类胚胎干细胞的心肌细胞

To date, the lack of a suitable human cardiac cell source has been the major setback in regenerating the human myocardium, either by cell-based ...

Generation of Mice Derived from Induced Pluripotent Stem Cells

诱导多能干细胞来源于小鼠的生成

The production of induced pluripotent stem cells (iPSCs) from somatic cells provides a means to create valuable tools for basic research and may also ...

The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

切片培养法继牙胚在植文化的发展

Explant culture allows manipulation of developing organs at specific time points&#160;and is therefore an important method for the developmental ...

Isolation, Culture, and Transplantation of Muscle Satellite Cells

分离，培养和移植肌卫星细胞

Muscle satellite cells are a stem cell population required for postnatal skeletal muscle development and regeneration, accounting for 2-5% of sublaminal ...