Name: anterior cervical discectomy and fusion in the ovine model
Uploaded: 2009-10-05T16:30:00
Description: Watch this Scientific Journal Video about Anterior Cervical Discectomy and Fusion in the Ovine Model at JoVE.com

<p class="jove_content">著者は、博士アンギボン、博士クリスティンマッカイとモナッシュアニマルサービスと生理学の動物ハウスですべてのスタッフに感謝。著者は、ジェレミーワインスタインとビデオ用Mcubeチームとアニメーションproduction.aに感謝</p>

<ol><li>羊は、24時間絶食が水を自由に摂取を許可されています</li><li>羊を秤量しています。 </li><li> Anaestheisaはthiopentone（20mg/kg）の静脈内注射により誘導される。 </li><li>予防的抗生物質の静脈内投与を与えることができる。 </li><li>気管内挿管は、サイズ7〜9カフ付き気管内チューブを使用して実行されます。 </li><li>麻酔は、2L毎分の流速で100％酸素では、イソフルラン、1-3％でmaintaintedている。 </li><li>観測が行?...

<ol>
	<li>Angevine, P. D., Arons, R. R., McCormick, P. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=National+and+regional+rates+and+variation+of+cervical+discectomy+with+and+without+anterior+fusion,+1990-1999.">National and regional rates and variation of cervical discectomy with and without anterior fusion, 1990-1999.</a> <em style='font-style: italic'>Spine</em>. <b>28</b>, 931-940 (2003).</li><li>Cloward, R. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+anterior+approach+for+removal+of+ruptured+cervical+disks.">The anterior approach for removal of ruptured cervical disks.</a> <em style='font-style: italic'>J Neurosurg</em>. <b>15</b>, 602-617 (1958).</li><li>Jacobs, W. C., Anderson, P. G., Limbeek, J., Willems, P. C., Pavlov, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Single+or+double-level+anterior+interbody+fusion+techniques+for+cervical+degenerative+disc+disease.">Single or double-level anterior interbody fusion techniques for cervical degenerative disc disease.</a> <em style='font-style: italic'>Cochrane Database Syst Rev</em>. , CD004958-CD004958 (2004).</li><li>Kandziora, F., Pflugmacher, R., Scholz, M., Schnake, K., Lucke, M., Schr&#246;der, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+between+sheep+and+human+cervical+spines:+an+anatomic,+radiographic,+bone+mineral+density,+and+biomechanical+study.">Comparison between sheep and human cervical spines: an anatomic, radiographic, bone mineral density, and biomechanical study.</a> <em style='font-style: italic'>Spine</em>. <b>26</b>, 1028-1037 (2001).</li><li>Patil, P. G., Turner, D. A., 	Pietrobon, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=National+trends+in+surgical+procedures+for+degenerative+cervical+spine+disease:+1990-2000.">National trends in surgical procedures for degenerative cervical spine disease: 1990-2000.</a> <em style='font-style: italic'>Neurosurgery</em>. <b>57</b>, 753-758 (2005).</li><li>Smith, G. W., Robinson, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+treatment+of+certain+cervical-spine+disorders+by+anterior+removal+of+the+intervertebral+disc+and+interbody+fusion.">The treatment of certain cervical-spine disorders by anterior removal of the intervertebral disc and interbody fusion.</a> <em style='font-style: italic'>J Bone Joint Surg Am</em>. <b>40-A</b>, 607-624 (1958).</li></ol>

<p class="jove_content">前頸部椎間板切除と融合を計画し、実行中に考慮すべき変動性のあるソースがあります。したがって、モデルの一貫性を保つために、動物は同じ年齢および品種でなければならない。我々は、2歳、雑種レスターメリノの羊を好む。正しく実行した場合、出血、感染、または死亡のような最小限の合併症が存在します。出血が閉じる前にチェック手順とhaemosatsisを通じて制御する必要がありま?...

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		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>Marcain</td>
<td>&nbsp;</td>
<td>AstraZeneca</td>
<td>&nbsp;</td>
<td>0/5% Bupivicaine hydrochloride solution for injection 100mg in 20 mls</td>
</tr>
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<td>Thiopentone</td>
<td>&nbsp;</td>
<td>Illium</td>
<td>&nbsp;</td>
<td>Thiopentone sodium (sterile powder for injection) 5g</td>
</tr>
<tr>
<td>Issoranne</td>
<td>&nbsp;</td>
<td>Baxter</td>
<td>&nbsp;</td>
<td>Isoflurane BP 100%</td>
</tr>
<tr>
<td>Chlorehxidine</td>
<td>&nbsp;</td>
<td>Jurox</td>
<td>&nbsp;</td>
<td>40mg/ml Chlorhexidine glucoronate</td>
</tr>
<tr>
<td>PVD Iodine Solution</td>
<td>&nbsp;</td>
<td>Apex</td>
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<td>Povidine Iodine 100mg/ml</td>
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anterior cervical discectomy and fusion in the ovine model

<p class="jove_content">前頸部椎間板切除術との融合（ACDF）は保守的な治療をしなかった患者における頸神経根及び/または脊髄症の最も一般的な外科手術です。<sup&gt; 1,5</sup&gt;。操作は最初クロワード法によって記述されて以来<sup&gt; 2</sup&gt;とスミスとロビンソン<sup&gt; 6</sup&gt; 1958年、技法、移植材料とインプラントの品種改良が行われています<sup&gt; 3</sup&gt;。特に、選択された患者の利益を得ることができる安全な骨誘導剤の必要性がある。羊のモデルは人間のカウンターパート、C3 / 4であり、最も類似したレベルに似ている解剖学的、生体力学、骨密度および放射線学的特性を有することが示されている<sup&gt; 4</sup&gt;。したがって、前臨床試験を行うことができる理想的なモデルです。特に、この方法論は、人間の脊椎手術における潜在的なアプリケーションのために、幹細胞を含むさまざまなデバイスと生物製剤を、評価に興味を持って研究者にも有用かもしれません。</p>

Watch this Scientific Journal Video about Anterior Cervical Discectomy and Fusion in the Ovine Model at JoVE.com

Anterior Cervical Discectomy and Fusion in the Ovine Model

<p class="jove_content">このビデオでは、ヒツジモデルで前頸部椎間板切除と融合の技法を示しています。</p>

羊モデルにおける前頸部椎間板切除術および融合

Anterior cervical discectomy and fusion (ACDF) is the most common surgical operation for cervical radiculopathy and/or myelopathy in patients who have ...

Research

JoVE Journal

Biology

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

単一粒子レベルでのウイルス融合動態の測定法

Membrane fusion is an essential step during entry of enveloped viruses into cells. Conventional fusion assays typically report on a large number of fusion ...

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

酵素による細胞融合アッセイを使用して、SNARE媒介膜融合の解析

The interactions of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor)  proteins on vesicles (v-SNAREs) and on target membranes ...

Evaluation of Muscle Function of the Extensor Digitorum Longus Muscle <em>Ex vivo</em> and Tibialis Anterior Muscle <em>In situ</em> in Mice

Evaluation of Muscle Function of the Extensor Digitorum Longus Muscle Ex vivo and Tibialis Anterior Muscle In situ in Mice

長指伸筋の筋機能の評価<em&gt;エクスビボ</em&gt;と前脛骨筋<em&gt;その場</em&gt;マウスの

Body  movements are mainly provided by mechanical function of skeletal muscle. Skeletal  muscle is composed of numerous bundles of myofibers that are ...

Using <em>Caenorhabditis elegans</em> as a Model System to Study Protein Homeostasis in a Multicellular Organism

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

The folding and assembly of proteins is essential for protein function, the long-term health of the cell, and longevity of the organism. Historically, the ...

The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

「せん断リング」の組み立てと応用：小説内皮軌道、単方向および定期的な流動のためのモデルとせん断応力

Deviations from normal levels and patterns of vascular fluid shear play important roles in vascular physiology and pathophysiology by inducing adaptive as ...

Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

マウス骨髄由来の樹状細胞とマクロファージの蛍光融合タンパク質の発現

Dendritic cells and macrophages are crucial cells that form the first line of defense against pathogens. They also play important roles in the initiation ...

Real Time <em>In Vivo</em> Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy

Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy

<em>生体内で</em>の実時間追跡レーザー顕微鏡による眼房内で胸腺細胞

The purpose of the method being presented is to show, for the first time, the transplant of newborn thymi into the anterior eye chamber of isogenic adult ...

Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

酵母におけるポリグルタミン毒性の試金を使用して融合パートナーの蛍光タンパク質の効果

For the investigation of protein localization and trafficking using live cell imaging, researchers often rely on fusing their protein of interest to a ...

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A

EYFP タグ付き CENP-A (EYFP-CENP-A) のユビキタス性を同定するための質量分析分析

Studying the structure and the dynamics of kinetochores and centromeres is important in understanding chromosomal instability (CIN) and cancer ...

Collection of Skeletal Muscle Biopsies from the Superior Compartment of Human Musculus Tibialis Anterior for Mechanical Evaluation

機械評価のためのヒト筋学の上室から骨格筋生検の収集

The mechanical properties of contracting skeletal fibers are crucial indicators of overall muscle health, function, and performance. Human skeletal muscle ...