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DOI :
10.3791/1549-v
•
12:19 min
October 7th, 2009
Chapters
0:00
Title
0:57
Introduction
1:29
Transfection of Plasmids for Production of Renilla-antigen Fusion Proteins
3:28
Harvesting Renilla-antigen Fusions
5:12
Preparing a Sera Master Plate
9:01
Measuring Luminescence and Data Analysis
6:30
LIPS Assay
10:49
LIPS Assay as a Method for Rapid and High Thoughput Sera Antigen Analysis
11:25
Conclusion
唇(荧光素酶免疫沉淀系统)技术方面进行了阐述。总体方法涉及表达嵌合基因编码融合海肾荧光素酶(中国人民大学)在哺乳动物细胞中的抗原。原油中国人民大学抗原的提取,然后准备,没有净化,量化抗体免疫检测。
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