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DOI :
10.3791/1555-v
•
8:49 min
December 24th, 2009
Chapters
0:00
Title
0:08
Introduction
0:19
Thawing and Plating hES Cells
5:12
Freezing hES Cells
8:15
Representative Results
8:36
Conclusion
自从詹姆斯汤姆森等人在1998年开发出一种技术,隔离,并在文化中成长的胚胎,已成为供以后使用,从冻结的股票解冻和扩大细胞的冷冻细胞进行常规胚胎干细胞培养中的重要程序。由于胚胎干细胞的冷冻和解冻的压力非常敏感,必须特别小心。在这里,我们表现出适当的技术迅速解冻胚胎干细胞从液氮股,对小鼠胚胎馈线细胞电镀,慢慢冻结他们长期储存。
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