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DOI :
10.3791/1561-v
•
20:47 min
August 13th, 2009
Chapters
0:07
Title
1:07
Introduction
2:18
Preparation of Thick Fresh Tissue Sections
5:22
Staining the Thick Sections with Tetramers
10:11
Preparing Thin Sections
12:17
Acquiring Confocal Microscope Images
17:56
Representative Images of Simultaneous Visualization by ISTH of Immune Effectors and Targets in Tissue Sections
19:50
Conclusion
その場テトラマー染色やin situハイブリダイゼーション(ISTH)で組み合わせる手法は可視化、マッピングおよび分析、そのウイルスに感染したターゲットへのウイルス特異的CD8 + T細胞の空間的な近接性の、及びこれらの免疫エフェクターと標的の間の量的関係を決定することが可能に感染の結果に。
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